Key features and details
- Mouse monoclonal [N25/35] to KIR2.3/HIR
- Suitable for: IHC-P, WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Product nameAnti-KIR2.3/HIR antibody [N25/35]
See all KIR2.3/HIR primary antibodies
DescriptionMouse monoclonal [N25/35] to KIR2.3/HIR
SpecificityDoes not cross react with Kir2.1 and Kir2.2
Tested applicationsSuitable for: IHC-P, WB, Flow Cytmore details
Species reactivityReacts with: Mouse, Rat, Human
- Rat brain lysate.
The clone number has been updated from S25-35 to N25/35, both clone numbers name the same antibody clone.
Previously labelled as KIR2.3.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab84822 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 0.1 - 1 µg/ml.|
|WB||Use a concentration of 1 - 10 µg/ml. Detects a band of approximately 45 kDa.|
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionThis receptor is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium.
Tissue specificityHeart, skeletal muscle, and several different brain regions including the hippocampus.
Sequence similaritiesBelongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ4 subfamily.
DomainThe Val/Gly/Ala/Pro stretch may have a functional role in the conductance or permeation properties.
Cellular localizationCell membrane. Cytoplasmic vesicle membrane. TAX1BP3 binding promotes dissociation of KCNJ4 from LIN7 famaly members and KCNJ4 internalization.
- Information by UniProt
- Hippocampal inward rectifier antibody
- Hippocampal inward rectifier potassium channel antibody
- HIR antibody
All lanes : Anti-KIR2.3/HIR antibody [N25/35] (ab84822) at 1 µg/ml
Lane 1 : Molecular weight marker
Lane 2 : Cell lysates prepared from human cell lines A431, A549, HCT116, HeLa, HEK293, HepG2, HL-60, HUVEC, Jurkat, MCF7, PC3 and T98G at 10 µg
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human hippocampus tissue fixed with Bouin's Fixative and paraffin-embedded staining KIR2.3/HIR with ab84822 at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.
Overlay histogram showing SH-SY5Y cells stained with ab84822 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84822, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab84822 has been referenced in 1 publication.
- Chen Y et al. MicroRNAs 221/222 and genistein-mediated regulation of ARHI tumor suppressor gene in prostate cancer. Cancer Prev Res (Phila) 4:76-86 (2011). WB ; Human . PubMed: 21071579