Product nameAnti-KIR2DL1 + KIR2DL2 antibody [EPR22492-2]
DescriptionRabbit monoclonal [EPR22492-2] to KIR2DL1 + KIR2DL2
Tested applicationsSuitable for: IHC-P, ICC/IF, Flow Cyt, IPmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Recombinant fragment within Human KIR2DL1 aa 1-250. The exact sequence is proprietary.
Database link: P43626
- WB: His-tagged human KIR2DL1 recombinant protein (aa1-245); His and LIF-tagged human KIR2DL2 recombinant protein (aa26-221). IHC-P: Human endometrium tissue. ICC/IF: NK-92 cells (treated with 5-aza-2-deoxycytidine (2µM) for 48h). Flow Cyt: Human PBMCs. IP: NK-92 (treated with 2µM 5-aza-2-deoxycytidine for 48h) whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab224696 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
Cellular localizationKIR2DL1: Cell membrane. KIR2DL2: Cell membrane.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NK-92 (human peripheral blood malignant non-Hodgkin's lymphoma cell line) cells (+/- treatment with 5-aza-2-deoxycytidine(2µM) for 48h) labeling KIR2DL1 + KIR2DL2 with ab224696 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in NK-92 cells treated with 5-aza-2-deoxycytidine(2uM) for 48h. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling KIR2DL1 + KIR2DL2 with ab224696 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in natural killer (NK) cells in human endometrium (PMID: 7749980) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
KIR2DL1 + KIR2DL2 were immunoprecipitated from 0.35 mg of NK-92 (human peripheral blood malignant non-Hodgkin's lymphoma cell line) (treated with 2μM 5-aza-2-deoxycytidine for 48h) whole cell lysate with ab224696 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab224696 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.
Lane 1: NK-92 (treated as above) whole cell lysate 10 μg (Input).
Lane 2: ab224696 IP in NK-92 (treated as above) whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab224696 in NK-92 (treated as above) whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 100 seconds.
This blot was developed using a higher sensitivity ECL substrate.
Flow cytometric analysis of human PBMCs (peripheral blood mononuclear cells) labeling KIR2DL1 + KIR2DL2 with ab224696 at 1/60 dilution (Right) compared with Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Left).
Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (ab150097), at 1/5000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG (Left) or ab224696 (Right). Then stained with anti-CD56 conjugated to BV421.
Gated on viable cells.
All lanes : Anti-KIR2DL1 + KIR2DL2 antibody [EPR22492-2] (ab224696) at 1/1000 dilution
Lane 1 : His-tagged human KIR2DL1 recombinant protein (aa1-245) 20 ng
Lane 2 : His and LIF-tagged human KIR2DL2 recombinant protein (aa26-221) 20 ng
Lane 3 : His-tagged human KIR2DL3 recombinant protein (aa1-245) 20 ng
Lane 4 : His-tagged human KIR2DS1 recombinant protein (aa22-221) 20 ng
Lane 5 : His-tagged human KIR2DS2 recombinant protein (aa22-221) 20 ng
Lane 6 : His-tagged human KIR2DS3 recombinant protein (aa22-221) 20 ng
Lane 7 : His-tagged human KIR2DS4 recombinant protein (aa22-221) 20 ng
Lane 8 : His-tagged human KIR2DS5 recombinant protein (aa22-221) 20 ng
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 39 kDa
Exposure time: 26 seconds
Blocking and dilution buffer: 5% NFDM/TBST.
ab224696 has not yet been referenced specifically in any publications.