Recombinant
RabMAb

Recombinant Anti-KIR2DL3 antibody [EPR22192] (ab225740)

Overview

  • Product name

    Anti-KIR2DL3 antibody [EPR22192]
    See all KIR2DL3 primary antibodies
  • Description

    Rabbit monoclonal [EPR22192] to KIR2DL3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human KIR2DL3 aa 150-250. The exact sequence is proprietary.
    Database link: P43628

  • Positive control

    • IHC-P: Human tonsil and endometrium tissues.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR22192
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab225740 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 38 kDa.

Target

  • Function

    Receptor on natural killer (NK) cells for HLA-C alleles (HLA-Cw1, HLA-Cw3 and HLA-Cw7). Inhibits the activity of NK cells thus preventing cell lysis.
  • Sequence similarities

    Belongs to the immunoglobulin superfamily.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD158 antigen like family member B2 antibody
    • CD158 antigen-like family member B2 antibody
    • CD158b antibody
    • CD158b2 antibody
    • GL183 antibody
    • KI2L3_HUMAN antibody
    • Killer cell immunoglobulin like receptor two domains long cytoplasmic tail 3 antibody
    • Killer cell immunoglobulin-like receptor 2DL3 antibody
    • Killer inhibitory receptor cl 2-3 antibody
    • Killer inhibitory receptor CL2 3 antibody
    • KIR 023GB antibody
    • KIR-023GB antibody
    • KIR-K7b antibody
    • KIR-K7c antibody
    • KIR023GB antibody
    • KIR2DL3 antibody
    • KIR2DS5 antibody
    • KIRCL23 antibody
    • MHC class I NK cell receptor antibody
    • Natural killer associated transcript 2 antibody
    • Natural killer cell inhibitory receptor KIR2DL3 antibody
    • Natural killer-associated transcript 2 antibody
    • NKAT antibody
    • NKAT-2 antibody
    • NKAT2 antibody
    • NKAT2a antibody
    • NKAT2b antibody
    • p58 antibody
    • p58 natural killer cell receptor clone CL 6 antibody
    • p58 natural killer cell receptor clone CL-6 antibody
    • p58 NK Receptor antibody
    • p58 NK receptor CL 6 antibody
    • p58 NK receptor CL-6 antibody
    • p58.2 MHC class I specific NK receptor antibody
    • p58.2 MHC class-I-specific NK receptor antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling KIR2DL3 with ab225740 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in natural killer (NK) cells in human endometrium (PMID: 19628706). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling KIR2DL3 with ab225740 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in natural killer (NK) cells in marginal zone of human tonsil (PMID: 19628706). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • All lanes : Anti-KIR2DL3 antibody [EPR22192] (ab225740) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
    Lane 2 : HEK-293T transfected with KIR2DL3 (WT) expression vector containing a myc-His-tag®, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 38 kDa
    Observed band size: 50-60 kDa
    why is the actual band size different from the predicted?



    Exposure time : 5.5 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

References

ab225740 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab225740.
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