Anti-G9P antibody (ab154386)
Key features and details
- Rabbit polyclonal to G9P
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-G9P antibody
See all G9P primary antibodies -
Description
Rabbit polyclonal to G9P -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human G9P aa 1-254.
Database link: Q99706 -
Positive control
- THP-1 whole cell lysate; HeLa cells; Human breast carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154386 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 41 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 41 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Receptor on natural killer (NK) cells for HLA-C alleles. Inhibits the activity of NK cells thus preventing cell lysis. -
Sequence similarities
Belongs to the immunoglobulin superfamily.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 3805 Human
- Omim: 604945 Human
- SwissProt: Q99706 Human
- Unigene: 651287 Human
- Unigene: 661219 Human
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Alternative names
- CD_antigen=CD158d antibody
- CD158 antigen like family member D antibody
- CD158 antigen-like family member D antibody
see all
Images
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Anti-G9P antibody (ab154386) at 1/1000 dilution + THP-1 whole cell lysate at 30 µg
Predicted band size: 41 kDa
10% SDS PAGE -
Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling G9P with ab154386 at 1/500 dilution (Green). Blue: Hoechst 33342 staining.
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Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling G9P with ab154386 at 1/250 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab154386 has been referenced in 3 publications.
- Zheng G et al. Interaction between HLA-G and NK cell receptor KIR2DL4 orchestrates HER2-positive breast cancer resistance to trastuzumab. Signal Transduct Target Ther 6:236 (2021). PubMed: 34158475
- Takei Y et al. Killer cell immunoglobulin-like receptor 2DL4 is expressed in and suppresses the cell growth of Langerhans cell histiocytosis. Oncotarget 8:36964-36972 (2017). PubMed: 28445138
- Ueshima C et al. The Killer Cell Ig-like Receptor 2DL4 Expression in Human Mast Cells and Its Potential Role in Breast Cancer Invasion. Cancer Immunol Res 3:871-80 (2015). PubMed: 25735953