Recombinant
RabMAb

Recombinant Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] (ab222100)

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Overview

  • Product name

    Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121]

  • Description

    Rabbit monoclonal [EPR22876-121] to KIR3DL1 + KIR3DS1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human KIR3DL1 aa 1-350. The exact sequence is proprietary.
    Database link: P43629, Q14943

  • Positive control

    • WB: NK-92 treated with 2µM 5-aza-2-deoxycytidine for 48 hours whole cell lysate, HEK-293T transfected with KIR3DL1 expression vector containing a myc-His-tag, whole cell lysate, HEK-293T transfected with KIR3DS1 expression vector containing a myc-His-tag, whole cell lysate; IHC-P: Human endometrium, KK-T lymphoma and spleen tissue.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22876-121

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab222100 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000.
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.

    • Target

    Images

    • Western blot - Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] (ab222100)
      Western blot - Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] (ab222100)
      All lanes : Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] (ab222100) at 1/1000 dilution

      Lane 1 : HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
      Lane 2 : HEK-293T transfected with KIR3DL1 expression vector containing a myc-His-tag®, whole cell lysate
      Lanes 3 & 5 : HEK-293T transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
      Lane 4 : HEK-293T transfected with KIR3DL2 expression vector containing a myc-His-tag®, whole cell lysate
      Lane 6 : HEK-293T transfected with KIR3DS1 expression vector containing a myc-His-tag®, whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Observed band size: 60,60-80 kDa
      why is the actual band size different from the predicted?


      Exposure time: 1 second


      Blocking and Dilution Buffer and concentration: 5% NFDM/TBST. KIR3DL1 and KIR3DS1 are glycoproteins. The molecular weight observed are consistent with what have been described in the literature (PMID:17202323, PMID:16423035).

    • Western blot - Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] (ab222100)
      Western blot - Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] (ab222100)
      All lanes : Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] (ab222100) at 1/1000 dilution

      Lane 1 : Untreated NK-92 (human malignant non-hodgkins lymphoma natural killer cell) whole cell lysate
      Lane 2 : NK-92 treated with 2uM 5-aza-2-deoxycytidine for 48 hours whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Observed band size: 40-70 kDa why is the actual band size different from the predicted?


      Exposure time: 10 seconds


      Blocking and Dilution Buffer and concentration: 5% NFDM/TBST. KIR3DL1 is a glycoprotein, its expression is induced after 5-aza-2-deoxycytidine treatment. The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID:12370356, PMID:16423035).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1 antibody [EPR22876-121] (ab222100)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1 antibody [EPR22876-121] (ab222100)

      Immunohistochemical analysis of paraffin-embedded human NK-T lymphoma tissue labeling KIR3DL1+KIR3DS1 with ab222100 at 1/500 dilution (1.438 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human NK-T lymphoma (PMID: 22140359) is observed. The section was incubated with ab222100 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1 antibody [EPR22876-121] (ab222100)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1 antibody [EPR22876-121] (ab222100)

      Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling KIR3DL1+KIR3DS1 with ab222100 at 1/500 dilution (1.438 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in some lymphocytes of human spleen (PMID: 22140359) is observed. The section was incubated with ab222100 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1 antibody [EPR22876-121] (ab222100)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1 antibody [EPR22876-121] (ab222100)

      Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling KIR3DL1+KIR3DS1 with ab222100 at 1/500 dilution (1.438 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in some stromal cells of human endometrium (PMID: 22140359) is observed. The section was incubated with ab222100 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    References

    ab222100 has not yet been referenced specifically in any publications.

    Publishing research using ab222100? Please let us know so that we can cite the reference in this datasheet.

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