RecombinantRabMAb

Recombinant Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] (ab222100)

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Western blot - Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] (ab222100)
  • Western blot - Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] (ab222100)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1 antibody [EPR22876-121] (ab222100)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1 antibody [EPR22876-121] (ab222100)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1 antibody [EPR22876-121] (ab222100)
  • Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] (ab222100)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22876-121] to KIR3DL1 + KIR3DS1
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121]

  • Description

    Rabbit monoclonal [EPR22876-121] to KIR3DL1 + KIR3DS1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human KIR3DL1 aa 1-350. The exact sequence is proprietary.
    Database link: P43629, Q14943

  • Positive control

    • WB: NK-92 treated with 2µM 5-aza-2-deoxycytidine for 48 hours whole cell lysate, HEK-293T transfected with KIR3DL1 expression vector containing a myc-His-tag, whole cell lysate, HEK-293T transfected with KIR3DS1 expression vector containing a myc-His-tag, whole cell lysate; IHC-P: Human endometrium, KK-T lymphoma and spleen tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22876-121

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab222100 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000.
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.

    • Target

    Images

    • Western blot - Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] (ab222100)
      Western blot - Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] (ab222100)
      All lanes : Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] (ab222100) at 1/1000 dilution

      Lane 1 : HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
      Lane 2 : HEK-293T transfected with KIR3DL1 expression vector containing a myc-His-tag®, whole cell lysate
      Lanes 3 & 5 : HEK-293T transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
      Lane 4 : HEK-293T transfected with KIR3DL2 expression vector containing a myc-His-tag®, whole cell lysate
      Lane 6 : HEK-293T transfected with KIR3DS1 expression vector containing a myc-His-tag®, whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Observed band size: 60,60-80 kDa
      why is the actual band size different from the predicted?


      Exposure time: 1 second


      Blocking and Dilution Buffer and concentration: 5% NFDM/TBST. KIR3DL1 and KIR3DS1 are glycoproteins. The molecular weight observed are consistent with what have been described in the literature (PMID:17202323, PMID:16423035).

    • Western blot - Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] (ab222100)
      Western blot - Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] (ab222100)
      All lanes : Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] (ab222100) at 1/1000 dilution

      Lane 1 : Untreated NK-92 (human malignant non-hodgkins lymphoma natural killer cell) whole cell lysate
      Lane 2 : NK-92 treated with 2uM 5-aza-2-deoxycytidine for 48 hours whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Observed band size: 40-70 kDa why is the actual band size different from the predicted?


      Exposure time: 10 seconds


      Blocking and Dilution Buffer and concentration: 5% NFDM/TBST. KIR3DL1 is a glycoprotein, its expression is induced after 5-aza-2-deoxycytidine treatment. The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID:12370356, PMID:16423035).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1 antibody [EPR22876-121] (ab222100)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1 antibody [EPR22876-121] (ab222100)

      Immunohistochemical analysis of paraffin-embedded human NK-T lymphoma tissue labeling KIR3DL1+KIR3DS1 with ab222100 at 1/500 dilution (1.438 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human NK-T lymphoma (PMID: 22140359) is observed. The section was incubated with ab222100 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1 antibody [EPR22876-121] (ab222100)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1 antibody [EPR22876-121] (ab222100)

      Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling KIR3DL1+KIR3DS1 with ab222100 at 1/500 dilution (1.438 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in some lymphocytes of human spleen (PMID: 22140359) is observed. The section was incubated with ab222100 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1 antibody [EPR22876-121] (ab222100)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1 antibody [EPR22876-121] (ab222100)

      Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling KIR3DL1+KIR3DS1 with ab222100 at 1/500 dilution (1.438 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in some stromal cells of human endometrium (PMID: 22140359) is observed. The section was incubated with ab222100 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] (ab222100)
      Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] (ab222100)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab222100? Please let us know so that we can cite the reference in this datasheet.

    ab222100 has not yet been referenced specifically in any publications.

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