Recombinant Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] (ab222100)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22876-121] to KIR3DL1 + KIR3DS1
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] -
Description
Rabbit monoclonal [EPR22876-121] to KIR3DL1 + KIR3DS1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human KIR3DL1 aa 1-350. The exact sequence is proprietary.
Database link: P43629, Q14943 -
Positive control
- WB: NK-92 treated with 2µM 5-aza-2-deoxycytidine for 48 hours whole cell lysate, HEK-293T transfected with KIR3DL1 expression vector containing a myc-His-tag, whole cell lysate, HEK-293T transfected with KIR3DS1 expression vector containing a myc-His-tag, whole cell lysate; IHC-P: Human endometrium, KK-T lymphoma and spleen tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22876-121 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Related Products
Applications
Our Abpromise guarantee covers the use of ab222100 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | 1/1000. | |
IHC-P | 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Cellular localization
KIR3DL1: Cell membrane. KIR3DS1: Cell membrane. -
Database links
- Entrez Gene: 3811 Human
- Entrez Gene: 3813 Human
- Omim: 604946 Human
- SwissProt: P43629 Human
- SwissProt: Q14943 Human
- Unigene: 645228 Human
- Unigene: 743496 Human
Images
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All lanes : Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] (ab222100) at 1/1000 dilution
Lane 1 : HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
Lane 2 : HEK-293T transfected with KIR3DL1 expression vector containing a myc-His-tag®, whole cell lysate
Lanes 3 & 5 : HEK-293T transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
Lane 4 : HEK-293T transfected with KIR3DL2 expression vector containing a myc-His-tag®, whole cell lysate
Lane 6 : HEK-293T transfected with KIR3DS1 expression vector containing a myc-His-tag®, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 60,60-80 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking and Dilution Buffer and concentration: 5% NFDM/TBST. KIR3DL1 and KIR3DS1 are glycoproteins. The molecular weight observed are consistent with what have been described in the literature (PMID:17202323, PMID:16423035).
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All lanes : Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] (ab222100) at 1/1000 dilution
Lane 1 : Untreated NK-92 (human malignant non-hodgkins lymphoma natural killer cell) whole cell lysate
Lane 2 : NK-92 treated with 2uM 5-aza-2-deoxycytidine for 48 hours whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 40-70 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsBlocking and Dilution Buffer and concentration: 5% NFDM/TBST. KIR3DL1 is a glycoprotein, its expression is induced after 5-aza-2-deoxycytidine treatment. The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID:12370356, PMID:16423035).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1 antibody [EPR22876-121] (ab222100)
Immunohistochemical analysis of paraffin-embedded human NK-T lymphoma tissue labeling KIR3DL1+KIR3DS1 with ab222100 at 1/500 dilution (1.438 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human NK-T lymphoma (PMID: 22140359) is observed. The section was incubated with ab222100 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1 antibody [EPR22876-121] (ab222100)
Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling KIR3DL1+KIR3DS1 with ab222100 at 1/500 dilution (1.438 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in some lymphocytes of human spleen (PMID: 22140359) is observed. The section was incubated with ab222100 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1 antibody [EPR22876-121] (ab222100)
Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling KIR3DL1+KIR3DS1 with ab222100 at 1/500 dilution (1.438 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in some stromal cells of human endometrium (PMID: 22140359) is observed. The section was incubated with ab222100 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab222100 has not yet been referenced specifically in any publications.