Anti-KIR3DL1 antibody [6D9A4] (ab233835)
![Western blot - Anti-KIR3DL1 antibody [6D9A4] (ab233835)](https://www.abcam.com/ps/products/233/ab233835/Images/ab233835-307733-anti-kir3dl1-antibody-6d9a4-western-blot.jpg "Western blot - Anti-KIR3DL1 antibody [6D9A4] (ab233835)")
Key features and details
- Mouse monoclonal [6D9A4] to KIR3DL1
- Suitable for: WB, Flow Cyt
- Reacts with: Rat, Human
- Isotype: IgG2b
Overview
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Product name
Anti-KIR3DL1 antibody [6D9A4]
See all KIR3DL1 primary antibodies -
Description
Mouse monoclonal [6D9A4] to KIR3DL1 -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment corresponding to Human KIR3DL1 aa 22-340. Expressed in HEK-293 cells.
Database link: P43629 -
Positive control
- WB: Recombinant human KIR3DL1 (aa22-340) protein; A431, Raji, SPC-A-1, K562, HEK-293, U937 and C6 cell lysates. Flow Cyt: Jurkat cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
6D9A4 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab233835 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/100 - 1/500. Predicted molecular weight: 49 kDa. | |
| Flow Cyt | 1/200 - 1/400. |
Target
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Function
Receptor on natural killer (NK) cells for HLA Bw4 allele. Inhibits the activity of NK cells thus preventing cell lysis. -
Sequence similarities
Belongs to the immunoglobulin superfamily.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 3811 Human
- Entrez Gene: 353253 Rat
- Omim: 604946 Human
- SwissProt: P43629 Human
- SwissProt: P83556 Rat
- Unigene: 645228 Human
- Unigene: 92529 Rat
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Alternative names
- AMB11 antibody
- CD158 antigen-like family member E antibody
- CD158e antibody
see all
Images
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Western blot - Anti-KIR3DL1 antibody [6D9A4] (ab233835)Anti-KIR3DL1 antibody [6D9A4] (ab233835) at 1/100 dilution + Recombinant human KIR3DL1 (aa 22-340) protein
Predicted band size: 49 kDaExpected recombinant MW is 65 kDa.
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![Western blot - Anti-KIR3DL1 antibody [6D9A4] (ab233835)](https://www.abcam.com/ps/products/233/ab233835/Images/ab233835-307734-anti-kir3dl1-antibody-6d9a4-western-blot.jpg)
Western blot - Anti-KIR3DL1 antibody [6D9A4] (ab233835)All lanes : Anti-KIR3DL1 antibody [6D9A4] (ab233835) at 1/100 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) cell lysate
Lane 2 : Raji (human Burkitt's lymphoma cell line) cell lysate
Lane 3 : SPC-A-1 cell lysate
Lane 4 : K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Lane 5 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 6 : U937 (human histiocytic lymphoma cell line) cell lysate
Lane 7 : C6 (rat glioma cell line) cell lysate
Predicted band size: 49 kDa -
![Flow Cytometry - Anti-KIR3DL1 antibody [6D9A4] (ab233835)](https://www.abcam.com/ps/products/233/ab233835/Images/ab233835-307735-anti-kir3dl1-antibody-6d9a4-flow-cytometry.jpg)
Flow Cytometry - Anti-KIR3DL1 antibody [6D9A4] (ab233835)Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling KIR3DL1 with ab233835 at 1/200 dilution (green) compared to a negative control (red).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab233835 has not yet been referenced specifically in any publications.
