Anti-KIR3DL1 antibody [6D9A4] (ab233835)

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Overview

  • Product name

    Anti-KIR3DL1 antibody [6D9A4]
    See all KIR3DL1 primary antibodies

  • Description

    Mouse monoclonal [6D9A4] to KIR3DL1

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, Flow Cytmore details

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Recombinant fragment corresponding to Human KIR3DL1 aa 22-340. Expressed in HEK-293 cells.
    Database link: P43629

  • Positive control

    • WB: Recombinant human KIR3DL1 (aa22-340) protein; A431, Raji, SPC-A-1, K562, HEK-293, U937 and C6 cell lysates. Flow Cyt: Jurkat cells.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Protein G purified

  • Purification notes

    Purified from tissue culture supernatant.

  • Clonality

    Monoclonal

  • Clone number

    6D9A4

  • Isotype

    IgG2b

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab233835 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 49 kDa.
Flow Cyt 1/200 - 1/400.

Target

  • Function

    Receptor on natural killer (NK) cells for HLA Bw4 allele. Inhibits the activity of NK cells thus preventing cell lysis.

  • Sequence similarities

    Belongs to the immunoglobulin superfamily.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • AMB11 antibody
      • CD158 antigen-like family member E antibody
      • CD158e antibody
      • CD158e antigen antibody
      • CD158E1 antibody
      • CD158E1/2 antibody
      • CD158E2 antibody
      • CL11 antibody
      • CL2 antibody
      • HLA-BW4-specific inhibitory NK cell receptor antibody
      • KI3L1_HUMAN antibody
      • killer cell immunoglobulin like receptor antibody
      • Killer cell immunoglobulin like receptor three domains , short cytoplasmic tail, 1 antibody
      • Killer cell immunoglobulin like receptor three domains long cytoplasmic tail 1 antibody
      • Killer cell immunoglobulin-like receptor 3DL1 antibody
      • KIR antibody
      • KIR antigen 3DL1 antibody
      • KIR G1 antibody
      • Kir3dl1 antibody
      • KIR3DS1 antibody
      • Kirl1 antibody
      • Kirl2 antibody
      • Krl1 antibody
      • MGC119726 antibody
      • MGC119728 antibody
      • MGC126589 antibody
      • MGC126591 antibody
      • MHC class I NK cell receptor antibody
      • Natural killer associated transcript 3 antibody
      • Natural killer cell inhibitory receptor antibody
      • Natural killer-associated transcript 3 antibody
      • NK receptor antibody
      • NK-associated transcript 10 antibody
      • NK-associated transcript 3 antibody
      • NK-associated transcript 3delIg1 antibody
      • NKAT-3 antibody
      • NKAT10 antibody
      • NKAT3 antibody
      • NKB1 antibody
      • NKB1B antibody
      • p70 killer cell inhibitory receptor antibody
      • p70 natural killer cell receptor clones CL 2/CL 11 antibody
      • p70 natural killer cell receptor clones CL-2/CL-11 antibody
      • p70 NK receptor CL-2/CL-11 antibody
      see all

    Images

    • Western blot - Anti-KIR3DL1 antibody [6D9A4] (ab233835)
      Western blot - Anti-KIR3DL1 antibody [6D9A4] (ab233835)
      Anti-KIR3DL1 antibody [6D9A4] (ab233835) at 1/100 dilution + Recombinant human KIR3DL1 (aa 22-340) protein

      Predicted band size: 49 kDa



      Expected recombinant MW is 65 kDa.

    • Western blot - Anti-KIR3DL1 antibody [6D9A4] (ab233835)
      Western blot - Anti-KIR3DL1 antibody [6D9A4] (ab233835)
      All lanes : Anti-KIR3DL1 antibody [6D9A4] (ab233835) at 1/100 dilution

      Lane 1 : A431 (human epidermoid carcinoma cell line) cell lysate
      Lane 2 : Raji (human Burkitt's lymphoma cell line) cell lysate
      Lane 3 : SPC-A-1 cell lysate
      Lane 4 : K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
      Lane 5 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
      Lane 6 : U937 (human histiocytic lymphoma cell line) cell lysate
      Lane 7 : C6 (rat glioma cell line) cell lysate

      Predicted band size: 49 kDa

    • Flow Cytometry - Anti-KIR3DL1 antibody [6D9A4] (ab233835)
      Flow Cytometry - Anti-KIR3DL1 antibody [6D9A4] (ab233835)

      Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling KIR3DL1 with ab233835 at 1/200 dilution (green) compared to a negative control (red).

    References

    ab233835 has not yet been referenced specifically in any publications.

    Publishing research using ab233835? Please let us know so that we can cite the reference in this datasheet.

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