Anti-Kir4.1/KCNJ10 antibody (ab240876)
Key features and details
- Rabbit polyclonal to Kir4.1/KCNJ10
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Kir4.1/KCNJ10 antibody
See all Kir4.1/KCNJ10 primary antibodies -
Description
Rabbit polyclonal to Kir4.1/KCNJ10 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide corresponding to Human Kir4.1/KCNJ10. (from the mid-protein)
Database link: P78508 -
Positive control
- ICC/IF: RKO cells. WB: Rat liver lysate. IHC: Human kidney
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General notes
This product was previously labelled as Kir4.1
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab240876 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/50. | |
| WB | 1/1000. | |
| ICC/IF | 1/100. |
Target
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Function
May be responsible for potassium buffering action of glial cells in the brain. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium (By similarity). In the kidney, together with KCNJ16, mediates basolateral K(+) recycling in distal tubules; this process is critical for Na(+) reabsorption at the tubules. -
Tissue specificity
Expressed in kidney (at protein level). -
Involvement in disease
Seizures, sensorineural deafness, ataxia, mental retardation, and electrolyte imbalance -
Sequence similarities
Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ10 subfamily. -
Cellular localization
Membrane. Basolateral cell membrane. In kidney distal convoluted tubules, located in the basolateral membrane where it colocalizes with KCNJ16. - Information by UniProt
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Database links
- Entrez Gene: 3766 Human
- Entrez Gene: 29718 Rat
- Omim: 602208 Human
- SwissProt: P78508 Human
- SwissProt: P49655 Rat
- Unigene: 408960 Human
- Unigene: 10196 Rat
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Alternative names
- inwardly rectifying subfamily J member 10 antibody
- ATP dependent inwardly rectifying potassium channel Kir4.1 antibody
- ATP sensitive inward rectifier potassium channel 10 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Kir4.1/KCNJ10 antibody (ab240876)
Immunocytochemistry/Immunofluorescence analysis of RKO (human colon carcinoma cell line) cells labeling Kir4.1/KCNJ10 using ab240876 (green) at 1/100 for 60 minutes at RT. Cells were fixed in 4% formaldehyde for 15 minutes at RT.
Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000, 1/5000 for 60 min RT, 5 min RT.
(A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab240876 (D) Composite.
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Western blot - Anti-Kir4.1/KCNJ10 antibody (ab240876)Anti-Kir4.1/KCNJ10 antibody (ab240876) at 1/1000 dilution + Rat liver lysate at 15 µg
Secondary
HRP-linked goat anti-rabbit IgG at 1/2000 dilution
Developed using the ECL technique.
Exposure time: 6 minutesBlocked with 5% skimmed milk in 1xTBST.
Incubated with primary antibody for 2 hours at RT and with secondary antibody for 1 hour at RT.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir4.1/KCNJ10 antibody (ab240876)Immunohistochemistry of human kidney tissue staining Kir4.1/KCNJ10 using ab240876 at (1/50) for 30 min at room temperature.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab240876 has not yet been referenced specifically in any publications.
