For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    kir41kcnj10-antibody-ab240876.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Neuroscience Neurotransmission Receptors / Channels Potassium Channels
Share by email

Anti-Kir4.1/KCNJ10 antibody (ab240876)

  • Datasheet
Submit a review Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunocytochemistry/ Immunofluorescence - Anti-Kir4.1/KCNJ10 antibody (ab240876)
  • Western blot - Anti-Kir4.1/KCNJ10 antibody (ab240876)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir4.1/KCNJ10 antibody (ab240876)

Key features and details

  • Rabbit polyclonal to Kir4.1/KCNJ10
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Rat, Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human Kir4.1/KCNJ10 protein (ab114456)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Kir4.1/KCNJ10 antibody
    See all Kir4.1/KCNJ10 primary antibodies
  • Description

    Rabbit polyclonal to Kir4.1/KCNJ10
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Kir4.1/KCNJ10. (from the mid-protein)
    Database link: P78508

  • Positive control

    • ICC/IF: RKO cells. WB: Rat liver lysate. IHC: Human kidney
  • General notes

     This product was previously labelled as Kir4.1

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: PBS, 50% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • Potassium Channels
    • Neuroscience
    • Sensory System
    • Auditory system

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Kir4.1/KCNJ10 protein (ab114456)

Applications

Our Abpromise guarantee covers the use of ab240876 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50.
WB 1/1000.
ICC/IF 1/100.

Target

  • Function

    May be responsible for potassium buffering action of glial cells in the brain. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium (By similarity). In the kidney, together with KCNJ16, mediates basolateral K(+) recycling in distal tubules; this process is critical for Na(+) reabsorption at the tubules.
  • Tissue specificity

    Expressed in kidney (at protein level).
  • Involvement in disease

    Seizures, sensorineural deafness, ataxia, mental retardation, and electrolyte imbalance
  • Sequence similarities

    Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ10 subfamily.
  • Cellular localization

    Membrane. Basolateral cell membrane. In kidney distal convoluted tubules, located in the basolateral membrane where it colocalizes with KCNJ16.
  • Target information above from: UniProt accession P78508 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3766 Human
    • Entrez Gene: 29718 Rat
    • Omim: 602208 Human
    • SwissProt: P78508 Human
    • SwissProt: P49655 Rat
    • Unigene: 408960 Human
    • Unigene: 10196 Rat
    • Alternative names

      • inwardly rectifying subfamily J member 10 antibody
      • ATP dependent inwardly rectifying potassium channel Kir4.1 antibody
      • ATP sensitive inward rectifier potassium channel 10 antibody
      • ATP-dependent inwardly rectifying potassium channel Kir4.1 antibody
      • ATP-sensitive inward rectifier potassium channel 10 antibody
      • BIRK10 antibody
      • Glial ATP dependent inwardly rectifying potassium channel KIR4.1 antibody
      • Inward rectifier K(+) channel Kir1.2 antibody
      • Inward rectifier K+ channel KIR1.2 antibody
      • Inwardly rectifying potassium channel Kir1.2 antibody
      • KCJ10_HUMAN antibody
      • KCNJ 10 antibody
      • Kcnj10 antibody
      • KCNJ13 PEN antibody
      • KIR1.2 antibody
      • KIR4.1 antibody
      • Potassium channel antibody
      • Potassium channel inwardly rectifying subfamily J member 10 antibody
      • Potassium inwardly rectifying channel subfamily J member 10 antibody
      • SESAME antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Kir4.1/KCNJ10 antibody (ab240876)
      Immunocytochemistry/ Immunofluorescence - Anti-Kir4.1/KCNJ10 antibody (ab240876)

      Immunocytochemistry/Immunofluorescence analysis of RKO (human colon carcinoma cell line) cells labeling Kir4.1/KCNJ10 using ab240876 (green) at 1/100 for 60 minutes at RT. Cells were fixed in 4% formaldehyde for 15 minutes at RT.

      Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000, 1/5000 for 60 min RT, 5 min RT.

      (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab240876 (D) Composite.

    • Western blot - Anti-Kir4.1/KCNJ10 antibody (ab240876)
      Western blot - Anti-Kir4.1/KCNJ10 antibody (ab240876)
      Anti-Kir4.1/KCNJ10 antibody (ab240876) at 1/1000 dilution + Rat liver lysate at 15 µg

      Secondary
      HRP-linked goat anti-rabbit IgG at 1/2000 dilution

      Developed using the ECL technique.

      Exposure time: 6 minutes


      Blocked with 5% skimmed milk in 1xTBST.

      Incubated with primary antibody for 2 hours at RT and with secondary antibody for 1 hour at RT.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir4.1/KCNJ10 antibody (ab240876)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir4.1/KCNJ10 antibody (ab240876)

      Immunohistochemistry of human kidney tissue staining Kir4.1/KCNJ10 using ab240876 at (1/50) for 30 min at room temperature. 

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab240876? Please let us know so that we can cite the reference in this datasheet.

    ab240876 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab240876.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.