Product nameAnti-Kir6.2/BIR antibody
See all Kir6.2/BIR primary antibodies
DescriptionRabbit polyclonal to Kir6.2/BIR
ab139389 detects endogenous levels of total Kir6.2/BIR protein.
Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to Human Kir6.2/BIR (internal sequence).
- Human brain, cortex tissue; 3T3 cell lysates; HeLa cells
This product was previously labelled as Kir6.2
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.88% Sodium chloride, 50% Glycerol
PBS is without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab139389 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 44 kDa.|
|IHC-P||Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/500.|
RelevanceATP-sensitive potassium (K(ATP)) channels are found in endocrine cells, neurons and both smooth and striated muscle, where they play an important role in controlling insulin secretion and vascular tone, and protect neurons under metabolic stress. Kir6.2 is a member of the inward rectifier potassium channel family, which is characterised by a greater tendency to allow potassium flow into the cell rather than out of it. It associates with the sulphonylurea receptor SUR1/ABCC8 to form a subfamily of K(ATP) channels that, when mutated or misregulated, are associated with forms of hyperinsulinemic hypoglycemia, neonatal diabetes, or pre-disposition to type 2 diabetes mellitus.
Cellular localizationCell Membrane; Multi-pass membrane protein
- ATP sensitive inward rectifier potassium channel 11 antibody
- Beta cell inward rectifier subunit antibody
- BIR antibody
All lanes : Anti-Kir6.2/BIR antibody (ab139389) at 1/500 dilution
Lane 1 : 3T3 cells lysates.
Lane 2 : 3T3 cells lysates. Also treated with the synthesized peptide.
Predicted band size: 44 kDa
Immunofluorescence analysis of HeLa cells labeling Kir6.2/BIR with ab139389 at a 1/100 dilution. The picture on the right is treated with the synthesized peptide.
Immunohistochemical analysis of formalin fixed, paraffin embedded Human brain cortex tissue labeling Kir6.2/BIR with ab139389 at 10 µg/ml.
ab139389 has not yet been referenced specifically in any publications.