Overview

  • Product name

  • Description

    Rabbit polyclonal to Kir6.2/BIR
  • Host species

    Rabbit
  • Specificity

    ab139389 detects endogenous levels of total Kir6.2/BIR protein.

  • Tested applications

    Suitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human Kir6.2/BIR (internal sequence).

  • Positive control

    • Human brain, cortex tissue; 3T3 cell lysates; HeLa cells
  • General notes

     This product was previously labelled as Kir6.2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab139389 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 44 kDa.
ELISA 1/10000.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/500.

Target

  • Relevance

    ATP-sensitive potassium (K(ATP)) channels are found in endocrine cells, neurons and both smooth and striated muscle, where they play an important role in controlling insulin secretion and vascular tone, and protect neurons under metabolic stress. Kir6.2 is a member of the inward rectifier potassium channel family, which is characterised by a greater tendency to allow potassium flow into the cell rather than out of it. It associates with the sulphonylurea receptor SUR1/ABCC8 to form a subfamily of K(ATP) channels that, when mutated or misregulated, are associated with forms of hyperinsulinemic hypoglycemia, neonatal diabetes, or pre-disposition to type 2 diabetes mellitus.
  • Cellular localization

    Cell Membrane; Multi-pass membrane protein
  • Database links

  • Alternative names

    • ATP sensitive inward rectifier potassium channel 11 antibody
    • Beta cell inward rectifier subunit antibody
    • BIR antibody
    • HHF 2 antibody
    • HHF2 antibody
    • IKATP antibody
    • Inward rectifier K(+) channel Kir6.2 antibody
    • Inwardly rectifying potassium channel KIR6.2 antibody
    • IRK 11 antibody
    • IRK11 antibody
    • KCNJ11 antibody
    • Kir 6.2 antibody
    • Kir6.2 antibody
    • MGC133230 antibody
    • PHHI antibody
    • Potassium channel inwardly rectifing subfamily J member 11 antibody
    • Potassium channel, inwardly rectifying subfamily J member 11 antibody
    • Potassium inwardly rectifying channel J11 antibody
    • TNDM 3 antibody
    • TNDM3 antibody
    see all

Images

  • All lanes : Anti-Kir6.2/BIR antibody (ab139389) at 1/500 dilution

    Lane 1 : 3T3 cells lysates.
    Lane 2 : 3T3 cells lysates. Also treated with the synthesized peptide.

    Predicted band size: 44 kDa

  • Immunofluorescence analysis of HeLa cells labeling Kir6.2/BIR with ab139389 at a 1/100 dilution. The picture on the right is treated with the synthesized peptide.

  • Immunohistochemical analysis of formalin fixed, paraffin embedded Human brain cortex tissue labeling Kir6.2/BIR with ab139389 at 10 µg/ml.

References

ab139389 has not yet been referenced specifically in any publications.

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