Overview

  • Product name

  • Description

    Goat polyclonal to Kir6.2/BIR
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Guinea pig
  • Immunogen

    Synthetic peptide corresponding to Mouse Kir6.2/BIR aa 30-42.
    Sequence:

    ERRARFVSKKGNC


    Database link: Q61743

  • Positive control

    • WB: Human muscle lysate. IHC-P: Human pancreas tissue.
  • General notes

     This product was previously labelled as Kir6.2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab223662 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 44 kDa).
IHC-P Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance

    ATP-sensitive potassium (K(ATP)) channels are found in endocrine cells, neurons and both smooth and striated muscle, where they play an important role in controlling insulin secretion and vascular tone, and protect neurons under metabolic stress. Kir6.2 is a member of the inward rectifier potassium channel family, which is characterised by a greater tendency to allow potassium flow into the cell rather than out of it. It associates with the sulphonylurea receptor SUR1/ABCC8 to form a subfamily of K(ATP) channels that, when mutated or misregulated, are associated with forms of hyperinsulinemic hypoglycemia, neonatal diabetes, or pre-disposition to type 2 diabetes mellitus.
  • Cellular localization

    Cell Membrane; Multi-pass membrane protein
  • Database links

  • Alternative names

    • ATP sensitive inward rectifier potassium channel 11 antibody
    • Beta cell inward rectifier subunit antibody
    • BIR antibody
    • HHF 2 antibody
    • HHF2 antibody
    • IKATP antibody
    • Inward rectifier K(+) channel Kir6.2 antibody
    • Inwardly rectifying potassium channel KIR6.2 antibody
    • IRK 11 antibody
    • IRK11 antibody
    • KCNJ11 antibody
    • Kir 6.2 antibody
    • Kir6.2 antibody
    • MGC133230 antibody
    • PHHI antibody
    • Potassium channel inwardly rectifing subfamily J member 11 antibody
    • Potassium channel, inwardly rectifying subfamily J member 11 antibody
    • Potassium inwardly rectifying channel J11 antibody
    • TNDM 3 antibody
    • TNDM3 antibody
    see all

Images

  • Paraffin embedded sections of human pancreas tissue were stained for Kir6.2/BIR with ab223662 at 3.8 μg/ml in immunohistochemical analysis.

  • Anti-Kir6.2/BIR antibody (ab223662) at 0.01 mg/ml + Human muscle lysate at 35 µg

    Developed using the ECL technique.

    Predicted band size: 44 kDa
    Observed band size: 45 kDa
    why is the actual band size different from the predicted?

References

ab223662 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab223662.
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