Overview

  • Product name

  • Description

    Rabbit polyclonal to Kir6.2/BIR
  • Host species

    Rabbit
  • Specificity

    Detects endogenous levels of total Kir6.2/BIR protein.

  • Tested applications

    Suitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human Kir6.2/BIR.

  • Positive control

    • Human brain tissue; extracts from HeLa cells and NIH-3T3 cells treated with heat shock; HeLa cells.
  • General notes

     This product was previously labelled as Kir6.2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab79171 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 44 kDa.
ELISA 1/10000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.

Target

  • Relevance

    ATP-sensitive potassium (K(ATP)) channels are found in endocrine cells, neurons and both smooth and striated muscle, where they play an important role in controlling insulin secretion and vascular tone, and protect neurons under metabolic stress. Kir6.2 is a member of the inward rectifier potassium channel family, which is characterised by a greater tendency to allow potassium flow into the cell rather than out of it. It associates with the sulphonylurea receptor SUR1/ABCC8 to form a subfamily of K(ATP) channels that, when mutated or misregulated, are associated with forms of hyperinsulinemic hypoglycemia, neonatal diabetes, or pre-disposition to type 2 diabetes mellitus.
  • Cellular localization

    Cell Membrane; Multi-pass membrane protein
  • Database links

  • Alternative names

    • ATP sensitive inward rectifier potassium channel 11 antibody
    • Beta cell inward rectifier subunit antibody
    • BIR antibody
    • HHF 2 antibody
    • HHF2 antibody
    • IKATP antibody
    • Inward rectifier K(+) channel Kir6.2 antibody
    • Inwardly rectifying potassium channel KIR6.2 antibody
    • IRK 11 antibody
    • IRK11 antibody
    • KCNJ11 antibody
    • Kir 6.2 antibody
    • Kir6.2 antibody
    • MGC133230 antibody
    • PHHI antibody
    • Potassium channel inwardly rectifing subfamily J member 11 antibody
    • Potassium channel, inwardly rectifying subfamily J member 11 antibody
    • Potassium inwardly rectifying channel J11 antibody
    • TNDM 3 antibody
    • TNDM3 antibody
    see all

Images

  • All lanes : Anti-Kir6.2/BIR antibody (ab79171) at 1/500 dilution

    Lane 1 : extracts from HeLa cells treated with heat shock
    Lane 2 : extracts from NIH3T3 cells treated with heat shock
    Lane 3 : extracts from NIH3T3 cells treated with heat shock with immunizing peptide at 10 µg

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 44 kDa
    Observed band size: 44 kDa

  • Immunohistochemical analysis of Kir6.2/BIR in paraffin embedded human brain tissue using a 1/50 dilution of ab79171, with (right) or without (left) pre treatment with the immunizing peptide.

  • Immunofluorescence analysis of Kir6.2/BIR in HeLa cells, using a 1/500 dilution of ab79171, with (right) or without (left) pre treatment with the immunizing peptide.

References

This product has been referenced in:

  • Gong XG  et al. Da-bu-yin-wan and qian-zheng-san to neuroprotect the mouse model of Parkinson's disease. Evid Based Complement Alternat Med 2014:729195 (2014). WB . Read more (PubMed: 25610480) »
  • Raeis-Dauvé V  et al. Increased expression of adenosine triphosphate-sensitive K+ channels in mitral dysfunction: mechanically stimulated transcription and hypoxia-induced protein stability? J Am Coll Cardiol 59:390-6 (2012). Read more (PubMed: 22133355) »
See all 2 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (heart)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
30 µg
Specification
heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Mar 13 2019

Answer

Thank you for your response. I am sorry to hear that the new replacement vial did not work. At that time when the new vial was shipped to you, there was no new batch available. This is to let you know that I have just contacted and asked our Account Department to raise a credit note for you - for the cost of one vial of ab79171. For your information, the internal reference note for this credit is CN18443. You can use this credit in the near future for any of the products which are in the catalogue. I hope this helps and if I can assist further, please do not hesitate to contact me.    

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Answer

Thank you for contacting Abcam Technical Team and for taking the time to provide some useful details of the experiments. I am very sorry to hear that your customer is having problems with this product. Though you have kindly provided some details, it would be much appreciated if I could get some more information which would help me identify the source of the problem. - Could you please specify the cell type of the mouse cells used for these experiments? - Total cell lysates or membrane preparations have been run on the gel? I look forward to hearing from you soon.

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