Recombinant Anti-KIRREL 3 antibody [EPR11631(2)] - BSA and Azide free (ab250077)
![Western blot - Anti-KIRREL 3 antibody [EPR11631(2)] - BSA and Azide free (ab250077)](https://www.abcam.com/ps/products/250/ab250077/Images/ab250077-398135-anti-kirrel-3-antibody-epr11631-2-bsa-and-azide-free-western-blot.jpg "Western blot - Anti-KIRREL 3 antibody [EPR11631(2)] - BSA and Azide free (ab250077)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11631(2)] to KIRREL 3 - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-KIRREL 3 antibody [EPR11631(2)] - BSA and Azide free
See all KIRREL 3 primary antibodies -
Description
Rabbit monoclonal [EPR11631(2)] to KIRREL 3 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Rat
-
Immunogen
Recombinant fragment within Human KIRREL 3. The exact sequence is proprietary.
Database link: Q8IZU9 -
General notes
ab250077 is the carrier-free version of ab178706.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR11631(2) -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Conjugation kits
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab250077 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 85 kDa.
|
| Notes |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 85 kDa. |
Target
-
Relevance
NEPH2 is a member of the nephrin like protein family, which includes NEPH1 (KIRREL; MIM 607428) and NEPH3 (KIRREL2; MIM 607762). The cytoplasmic domains of these proteins interact with the C terminus of podocin (NPHS2; MIM 604766), and the genes are expressed in kidney podocytes, cells involved in ensuring size and charge selective ultrafiltration (Sellin et al., 2003 [PubMed 12424224]). -
Cellular localization
Cell membrane; Single-pass type I membrane protein -
Database links
- Entrez Gene: 84623 Human
- Entrez Gene: 315546 Rat
- Omim: 607761 Human
- SwissProt: Q8IZU9 Human
-
Alternative names
- KIAA1867 antibody
- Kin of IRRE like 3 (Drosophila) antibody
- Kin of IRRE like 3 antibody
see all
Images
-
Western blot - Anti-KIRREL 3 antibody [EPR11631(2)] - BSA and Azide free (ab250077)All lanes : Anti-KIRREL 3 antibody [EPR11631(2)] (ab178706) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : SH-SY5Y lysate
Lane 3 : K562 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 85 kDaThis data was developed using ab178706, the same antibody clone in a different buffer formulation.
-
![Anti-KIRREL 3 antibody [EPR11631(2)] - BSA and Azide free (ab250077)](https://www.abcam.com/ps/products/250/ab250077/Images/ab250077-2-benefits-of-recombinant-antibodies.png)
Anti-KIRREL 3 antibody [EPR11631(2)] - BSA and Azide free (ab250077)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
Certificate of Compliance
References (0)
ab250077 has not yet been referenced specifically in any publications.
