Overview

  • Product name
  • Description
    Rabbit polyclonal to Kisspeptin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide near the N terminus of the full-length mouse KISS1 protein.

  • Positive control
    • Jurkat cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab19028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 15 kDa.Can be blocked with Kisspeptin peptide (ab37702).

Target

  • Function
    Metastasis suppressor protein in malignant melanomas and in some breast cancers. May regulate events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. Generates a C-terminally amidated peptide, metastin which functions as the endogenous ligand of the G-protein coupled receptor GPR54. Activation of the receptor inhibits cell proliferation and cell migration, key characteristics of tumor metastasis. Kp-10 is a decapeptide derived from the primary translation product, isolated in conditioned medium of first trimester trophoblast. Kp-10, but not other kisspeptins, increased intracellular Ca(2+) levels in isolated first trimester trophoblasts. Kp-10 is a paracrine/endocrine regulator in fine-tuning trophoblast invasion generated by the trophoblast itself. The receptor is also essential for normal gonadotropin-released hormone physiology and for puberty. The hypothalamic KiSS1/GPR54 system is a pivotal factor in central regulation of the gonadotropic axis at puberty and in adulthood.
  • Tissue specificity
    Very high expression in placenta, with the next highest level in testis and moderate levels in pancreas, liver, small intestine and brain at much lower levels. Expression levels increased in both early placentas and molar pregnancies and are reduced in choriocarcinoma cells. Expressed at higher levels in first trimester trophoblasts than at term of gestation, but only expressed in the villous trophoblast.
  • Sequence similarities
    Belongs to the KISS1 family.
  • Post-translational
    modifications
    Processed by MMP2 and MMP9.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • KISS 1 antibody
    • KiSS 1 metastasis suppressor antibody
    • Kiss1 antibody
    • KISS1_HUMAN antibody
    • Kisspeptin 1 antibody
    • Kisspeptin 10 antibody
    • Kisspeptin 13 antibody
    • Kisspeptin 14 antibody
    • Kisspeptin antibody
    • Kisspeptin-1 antibody
    • Kisspeptin-10 antibody
    • Kisspeptin-54 antibody
    • Kisspeptin1 antibody
    • Malignant melanoma metastasis suppressor antibody
    • Metastasis suppressor KiSS 1 antibody
    • Metastasis suppressor KiSS1 antibody
    • Metastin antibody
    • MGC39258 antibody
    see all

Images

  • Anti-Kisspeptin antibody (ab19028) at 1 µg/ml + Jurkat cell lysate (30ug)

    Predicted band size: 15 kDa
    Observed band size: 15,35 kDa
    why is the actual band size different from the predicted?

  • ab19028 staining human breast cancer cells. 20 µg of protein were loaded in to each lane. The primary antibody was diluted to 1 µg/ml and incubated with the blot for 15 hours at 4°C. A HRP conjugated donkey anti-rabbit IgG antibody, diluted 1/5000, was used as the secondary.

    See Abreview

References

This product has been referenced in:
  • Zhai L  et al. Downregulation of leptin receptor and kisspeptin/GPR54 in the murine hypothalamus contributes to male hypogonadism caused by high-fat diet-induced obesity. Endocrine 62:195-206 (2018). Read more (PubMed: 29948931) »
  • Fernandois D  et al. Kisspeptin is involved in ovarian follicular development during aging in rats. J Endocrinol 228:161-70 (2016). Read more (PubMed: 26698566) »
See all 8 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
Yes - Triton X-100
Specification
brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Paraformaldehyde

Doyeon Kim

Verified customer

Submitted Dec 07 2017

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (brain)
Specification
brain
Fixative
Methanol
Antigen retrieval step
None
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Dr. Nitsan Kozlovsky

Verified customer

Submitted Oct 01 2012

Answer

Thank you for contacting us.
We would be interested in this information. I have therefore generated the testing discount as below:
testing discount: xxxxxx expiration date: 14-Nov-12
The code becomes active once the customer has submitted and Abreview for ab19028 used in IHC-Fr with their rat samples. More information on the testing discount code can be found from the following link:
www.abcam.com/collaborationdiscount
I hope this information has been of help. If you have any further questions, please do not hesitate to ask.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Breast cancer cells)
Loading amount
20 µg
Specification
Breast cancer cells
Treatment
overexpressing DNAJB6
Gel Running Conditions
Reduced Denaturing (15%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Dr. Aparna Mitra

Verified customer

Submitted Jul 11 2008

Question
Answer

Thank you for your enquiry. I have been informed by our source for this antibody that the proprietary peptide used to generate this antibody is from the N-terminus of the secreted protein, so it will recognize the secreted as well as the intracellular precursor form. If you have any other questions, please do not hesitate to contact us.

Read More

Answer

I'm sorry about the confusion. The NP_839991 (PubMed/Search protein) provides the mouse Kisspeptin amino acid sequence. I've now included this information below. Based on this, amino acid 14-27/the immunogen sequence would be "cvatygeplakvap". Please let me know if this helps with interpretation of your results. 1 mismaswqll lllcvatyge plakvaplvk pgstgqqsgp qelvnaweke sryaeskpgs 61 aglrarrssp cppvegpagr qrplcasrsr lipaprgavl vqrekdlsty nwnsfglryg 121 rrqaaraarg

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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