Anti-KIST/KIS antibody (ab237501)
Key features and details
- Rabbit polyclonal to KIST/KIS
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KIST/KIS antibody -
Description
Rabbit polyclonal to KIST/KIS -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Orangutan -
Immunogen
Recombinant fragment corresponding to Human KIST/KIS aa 150-400.
Database link: Q8TAS1 -
Positive control
- IHC-P: Human prostate tissue. ICC/IF: PC-3 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity greater than 95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab237501 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
1/50 - 1/200.
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IHC-P |
1/20 - 1/200.
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Notes |
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ICC/IF
1/50 - 1/200. |
IHC-P
1/20 - 1/200. |
Target
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Function
Upon serum stimulation, phosphorylates CDKN1B/p27Kip1, thus controlling CDKN1B subcellular location and cell cycle progression in G1 phase. May be involved in trafficking and/or processing of RNA. -
Tissue specificity
Widely expressed, with highest levels in skeletal muscle, kidney, placenta and peripheral blood leukocytes. -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 protein kinase domain.
Contains 1 RRM (RNA recognition motif) domain. -
Cellular localization
Nucleus. Mostly nuclear. - Information by UniProt
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Database links
- Entrez Gene: 127933 Human
- Entrez Gene: 16589 Mouse
- Entrez Gene: 100172072 Orangutan
- Entrez Gene: 246332 Rat
- Omim: 608849 Human
- SwissProt: Q8TAS1 Human
- SwissProt: P97343 Mouse
- SwissProt: Q5RCY1 Orangutan
see all -
Alternative names
- DKFZp434C1613 antibody
- FLJ23015 antibody
- kinase interacting with leukemia-associated gene (stathmin) antibody
see all
Images
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Paraffin-embedded human prostate tissue stained for KIST/KIS using ab237501 at 1/100 dilution in immunohistochemical analysis.
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PC-3 (human prostate adenocarcinoma cell line) cells stained for KIST/KIS (green) using ab237501 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab237501 has not yet been referenced specifically in any publications.