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Antibody arrays are an ideal tool to identify the key changes in a protein pathway or network. Cytokine arrays particularly are used extensively in immunology research.
Antibody arrays are multiplex immunoassays that use the ELISA sandwich antibody pair principle, allowing multiple proteins to be detected within one experiment. Multiple capture antibodies are pre-spotted on to a solid surface, sample is applied and analytes are then captured, addition of detection antibodies complete the sandwich complex.
Choose our quantitative antibody arrays on glass slides to quantitate up to 40 proteins per array, with as little as 50 µL of sample, and analyze with a microarray laser slide scanner.
Examples of our popular antibody arrays include: cytokine array ab133997, our human cytokine array (membrane antibody array) that measures 42 different cytokines; ab134001, our human apoptosis membrane antibody array and ab197419, our human angiogenesis quantitative antibody array. Browse these and others using the links to the left.
|Semi-quantitative membrane antibody arrays||Quantitative slide antibody arrays|
|Array format||1 array per nitrocellulose membrane||16 arrays per glass slide|
|Assay type||Semi-quantitative||Quantitative (8 arrays per slide are used for a standard curve, protein standards are supplied)|
|Sample volume||200 µL - 1 mL||50 - 100 µL|
|Detection||Chemiluminescence||Fluorescence (550 nm, Cy3 equivalent dye)|
|Reproducibility||Only comparable within a single experiment||CV < 15-20%|
|Cost||More expensive per kit, similar price per sample|
|Proteins per array||Up to 96||Up to 40 (can easily process multiple arrays in parallel to measure up to 400 proteins)|
|No. of samples||Suitable for up to ~20 samples||Easily processed in parallel for many samples & suitable for automation|
|Instrumentation||X-ray film, CCD camera, gel doc or Licor® Odyssey Imager||Laser slide scanner|