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Antibody arrays

Related

  • Browse quantitative cytokine arrays and other antibody arrays
    • Browse membrane cytokine arrays and other antibody arrays
        • Human analyte comparison for cytokine arrays and other antibody arrays
          • Mouse analyte comparison for cytokine arrays and other antibody arrays

            ​​We offer cytokine arrays and other antibody arrays for more than 400 cytokines, chemokines, and other proteins.

            Arrays are available for human, mouse, rat and other species.

            ​​Antibody arrays are an ideal tool to identify the key changes in a protein pathway or network. Cytokine arrays particularly are used extensively in immunology research.

            ​​​​​Antibody arrays are multiplex immunoassays that use the ELISA sandwich antibody pair principle, allowing multiple proteins to be detected within one experiment. Multiple capture antibodies are pre-spotted on to a solid surface, the sample is applied and analytes are then captured, the addition of detection antibodies complete the sandwich complex.

            ​​​​​​Choose our quantitative antibody arrays on glass slides to quantitate up to 40 proteins per array, with as little as 50 µL of sample, and analyze with a microarray laser slide scanner.

            Discover more about quantitative antibody arrays


            • Choose our membrane antibody cytokine arrays to quickly screen and profile samples across up to 96 proteins per array, with 200 µL–1 mL of sample, and analyze just like a chemiluminescent western blot.
            Discover more about membrane antibody arrays

            Examples of our popular antibody arrays include: cytokine array ab133997, our human cytokine array (membrane antibody array) that measures 42 different cytokines; ab134001, our human apoptosis membrane antibody array and ab197419, our human angiogenesis quantitative antibody array. Browse these and others using the links to the left.  





            Semi-quantitative membrane antibody arraysQuantitative slide antibody arrays
            Array format1 array per nitrocellulose membrane16 arrays per glass slide
            Assay typeSemi-quantitativeQuantitative (8 arrays per slide are used for a standard curve, protein standards are supplied)
            Sample volume200 µL - 1 mL50 - 100 µL
            DetectionChemiluminescenceFluorescence (550 nm, Cy3 equivalent dye)
            Sensitivitypg/mlpg/ml-ng/ml
            ReproducibilityOnly comparable within a single experimentCV < 15-20%
            Cost
            More expensive per kit, similar price per sample
            Proteins per arrayUp to 96Up to 40 (can easily process multiple arrays in parallel to measure up to 400 proteins)
            No. of samplesSuitable for up to ~20 samplesEasily processed in parallel for many samples & suitable for automation
            InstrumentationX-ray film, CCD camera, gel doc or Licor® Odyssey ImagerLaser slide scanner



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