Antibody binding affinities of Protein A, Protein G, Protein L and jacalin
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Our guide will help you choose the most suitable antibody binding protein for your antibody purification, immunoprecipitation or chromatin immunoprecipitation experiment.
Antibody binding proteins such as Protein A and Protein G are often used in antibody purification and in applications such as immunoprecipitation (IP) and chromatin immunoprecipitation (ChIP). Each antibody binding protein varies in its ability to bind to antibodies of different subtypes and species. It is therefore important that you match the binding specificity of the immobilized antibody binding protein to the species and antibody subtype of the antibody that you wish to capture. Here, we list the antibody binding properties of Protein A, Protein G, Protein A/G, Protein L and jacalin.
View our beads/resins and columns attached to antibody binding proteins in the table below.
Find out more about affinity purification here.
Antibody binding properties of Protein A, Protein G, Protein A/G and Protein L by antibody species and subtype
Key: +++ strong binding; ++ medium binding; + weak binding; - no binding; ? undetermined
| Species | Antibody subtype | Protein A | Protein G | Protein A/G | Protein L*** |
| Human | Total IgG | +++ +++ +++ + +++ + - + + + + | +++ +++ +++ +++ +++ - - - - + - | +++ +++ +++ +++ +++ + - + + + + | +++ +++ +++ +++ +++ +++ +++ +++ +++ +++ +++ |
| Mouse | Total IgG IgM IgG1 IgG2a IgG2b IgG3 | +++ - + +++ +++ +++ | +++ - ++ +++ +++ +++ | +++ - ++ +++ +++ +++ | +++ +++ +++ +++ +++ +++ |
| Rat | Total IgG IgG1 IgG2a IgG2b IgG2c | + + - - +++ | ++ ++ +++ + +++ | ++ ++ +++ + +++ | +++ +++ +++ + +++ |
| Rabbit | Total IgG | +++ | +++ | +++ | + |
| Goat | Total IgG | + | +++ | +++ | - |
| Sheep | Total IgG | + | +++ | +++ | - |
| Guinea pig | Total IgG | +++ | + | +++ | ? |
| Hamster | Total IgG | ++ | ++ | ++ | +++ |
| Donkey | Total IgG | ++ | +++ | +++ | ? |
| Pig | Total IgG | +++ | + | +++ | +++ |
| Dog | Total IgG | +++ | + | +++ | ? |
| Cat | Total IgG | +++ | + | +++ | ? |
| Chicken | Total IgY | - | - | - | - |
| Cow | Total IgG | + | +++ | +++ | - |
| Horse | Total IgG | + | +++ | +++ | ? |
***Protein L binding is restricted to antibodies that contain the right subtypes of kappa light chains. Protein L binds to human VκI, VκIII and VκIV subtypes but not to VκII, and only binds to mouse VκI-containing antibodies.
Antibody binding properties of jacalin by antibody species and subtype
| Species | Antibody subtype | Jacalin |
| Human | Total IgG IgG1 IgG2 IgG3 IgG4 IgM IgD IgA1 IgA2 Fab scFv Kappa light chain Lambda light chain | - - - - - - ++ +++ + +/- ? - - |
| Mouse | Total IgG IgA | - - |
| Rat | Total IgG IgA | ? - |
| Rabbit | IgA | + |
| Goat | IgA | - |
| Sheep | IgA | ? |
| Guinea pig | IgA | - |
| Hamster | IgA | ? |
| Donkey | IgA | ? |
| Pig | IgA | - |
| Dog | IgA | - |
| Cat | IgA | ? |
| Chicken | IgY | ? |
| Cow | IgA | - |
| Horse | IgA | - |
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