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Antibody binding affinities of Protein A, Protein G, Protein L and jacalin

Related

  • Overview of affinity purification
    • Antibody purification and concentration kits
      • RNA immunoprecipitation (RIP) protocol

        ​

        Our guide will help you choose the most suitable antibody binding protein for your antibody purification, immunoprecipitation or chromatin immunoprecipitation experiment.

        Antibody binding proteins such as Protein A and Protein G are often used in antibody purification and in applications such as immunoprecipitation (IP) and chromatin immunoprecipitation (ChIP). Each antibody binding protein varies in its ability to bind to antibodies of different subtypes and species. It is therefore important that you match the binding specificity of the immobilized antibody binding protein to the species and antibody subtype of the antibody that you wish to capture. Here, we list the antibody binding properties of Protein A, Protein G, Protein A/G, Protein L and jacalin.

        View our beads/resins and columns attached to antibody binding proteins in the table below.

        Find out more about affinity purification here.

        Antibody binding properties of Protein A, Protein G, Protein A/G and Protein L by antibody species and subtype

        Key: +++ strong binding; ++ medium binding; + weak binding; - no binding; ? undetermined

        SpeciesAntibody subtypeProtein AProtein GProtein A/GProtein L***
        Human

        Total IgG
        ​IgG1
        IgG2
        ​IgG3
        ​IgG4
        ​IgM
        ​IgD
        ​IgA1
        IgA2
        Fab
        ​scFv​

        +++
        +++
        +++
        +
        +++
        +
        -
        ​+
        +
        +​
        +
        +++
        +++
        +++
        +++
        +++
        -
        -
        -
        -
        +
        -
        +++
        +++
        +++
        +++
        +++
        +
        -
        +
        +
        +
        +
        +++
        ​+++
        +++
        +++
        +++
        +++
        +++
        +++
        +++
        +++
        +++
        MouseTotal IgG
        ​IgM
        ​IgG1
        ​IgG2a
        ​IgG2b
        ​IgG3

        +++
        -
        +
        +++
        +++
        +++
        +++
        -
        ++
        +++
        +++
        +++
        +++
        -
        ++
        +++
        +++
        +++
        +++
        +++
        +++
        +++
        +++
        ​+++
        RatTotal IgG
        IgG1
        IgG2a
        ​​IgG2b
        IgG2c

        +
        +
        -
        -
        +++

        ++
        ++
        +++
        +
        +++
        ++
        ++
        +++
        +
        +++
        +++
        +++
        +++
        +
        ​+++
        RabbitTotal IgG++++++++++
        GoatTotal IgG+++++++-
        SheepTotal IgG+++++++-
        Guinea pigTotal IgG+++++++?
        HamsterTotal IgG+++++++++
        DonkeyTotal IgG++++++++?
        PigTotal IgG++++++++++
        DogTotal IgG+++++++?
        CatTotal IgG+++++++?
        ChickenTotal IgY----
        CowTotal IgG+++++++-
        HorseTotal IgG+++++++?

        ***Protein L binding is restricted to antibodies that contain the right subtypes of kappa light chains. Protein L binds to human VκI, VκIII and VκIV subtypes but not to VκII, and only binds to mouse VκI-containing antibodies.


        ​Antibody binding properties of jacalin by antibody species and subtype

        SpeciesAntibody subtypeJacalin
        HumanTotal IgG
        ​IgG1
        IgG2
        ​IgG3
        IgG4
        ​IgM
        ​IgD
        ​IgA1
        ​IgA2
        ​Fab
        ​scFv
        ​Kappa light chain
        Lambda light chain
        -
        -
        -
        -
        -
        -
        ++
        +++
        +
        +/-
        ?
        -
        ​-
        MouseTotal IgG
        ​IgA
        -
        -
        RatTotal IgG
        ​IgA
        ?
        -
        RabbitIgA+
        GoatIgA-
        SheepIgA?
        Guinea pigIgA-
        HamsterIgA?
        DonkeyIgA?
        PigIgA-
        DogIgA-
        CatIgA?
        ChickenIgY?
        CowIgA-
        HorseIgA-

        Sepharose ® is a registered trademark of GE Healthcare. 

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