Antibody conjugation kits

The fast way to label your antibody.

• Our antibody conjugation kits allow you to quickly add the label of your choice onto your antibody (or other protein)
• Choose from labels including enzymes, fluorochromes, biotin/streptavidin, oligonucleotides, and gold
• Antibodies are immediately ready for use in flow cytometry, western blot, ELISA, IHC and more
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​​Overview

• Antibody conjugation kit advantages
• Antibody and buffer requirements for conjugation
• What other researchers say
• Available conjugation kits
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​Antibody conjugation kit advantages

​Our antibody conjugation kits are quick and easy to use:

• Less than one minute hands-on time
• Conjugated antibody ready in as little as 20 minutes (3 hours for some kits)
• One-step labeling method with no separation steps, giving 100% antibody recovery
• Can be used with 10 µg of antibody to over 1 mg
  
  

​​​Requirements for antibody conjugation 

The purity and concentration of your antibody are important for successful conjugation:

• Only purified antibodies are suitable for conjugation (ie not crude serum or tissue culture supernatant)
• Certain buffer constituents are incompatible with the labeling reaction (see conjugation kit datasheet for details)
• The antibody should be at least 0.5 mg/mL concentration

If your antibody does not meet these requirements, you may need to purify or concentrate your antibody using our purification and concentration kits.
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What other researchers say

Researchers and assay developers have used over 20,000 Abcam conjugation kits since 2011.

"Using the Biotin conjugation kit was very easy and quick. We achieved excellent results in an antibody labeling reaction. The epitope-recognition of the antibody was not affected and detection via streptavidin-HRP is very sensitive"
Researcher review for Biotin conjugation kit 

"The R-Phycoerythrin conjugation kit was extremely easy to use and effective despite the very small amount of antibody labeled (60µg). "
Researcher review for ab102918 R-PE conjugation kit

"The kit work[ed] fantastically with some store bought, pure, concentrated antibody! It actually gave better results than our previous method of using a conjugated secondary antibody when used in Flow Cytometry after doing an Intracellular Stain."
Researcher review for ab102884 FITC Conjugation Kit

"Use[d] the product Biotinylation Kit/Biotin Conjugation Kit (Fast, Type B) for biotinylation of Protein X. The product not only successfully biotinylated the protein, the protein can also be co-immunoprecipitated with streptavidin coated magnetic beads from cell lysate."
Researcher review for ab201796 Biotinylation Kit

"The conjugation kit was very easy to use and gave a very nice signal."
Researcher review for ab201800 Dylight 550 Conjugation Kit

"Improved staining for mouse-on-mouse immunofluorescence... Mouse Anti-CCX4 (ab27591) labeled with Dylight 550 Fast Conjugation Kit (ab201800)"

Researcher review for ab201800 Dylight 550 Conjugation Kit

See below for our list of conjugation kits for enzyme labels, fluorescent dyes, fluorescent proteins and tandem dyes, biotin / streptavidin, oligonucleotides, and gold.

Enzyme labels

• Alkaline phosphatase (AP) conjugation kit 
• Glucose oxidase (GOx) conjugation kit
• Horse radish peroxidase (HRP) conjugation kit

Fluorescent dyes

• Alexa Fluor^® 488 conjugation kit
  
• Alexa Fluor^® 555 conjugation kit
  
• Alexa Fluor^® 568 conjugation kit
  
• Alexa Fluor^® 594 conjugation kit
  
• Alexa Fluor^® 647 conjugation kit
  
• Alexa Fluor^® 700 conjugation kit
• AMCA conjugation kit
• Atto 390 conjugation kit
• Atto 488 conjugation kit
• Atto 565 conjugation kit
• Atto 633 conjugation kit
• Cy3^® conjugation kit
• Cy5^® conjugation kit
• Cy5.5^® conjugation kit
  
• Dylight^® 350 conjugation kit
• Dylight^® 405 conjugation kit
• Dylight^® 488 conjugation kit
• Dylight^® 550 conjugation kit
• Dylight^® 594 conjugation kit
• ​Dylight^® 633 conjugation kit
• Dylight^® 650 conjugation kit
• Dylight^® 680 conjugation kit
• Dylight^® 755 conjugation kit
• Dylight^® 800 conjugation kit
• FITC conjugation kit
• ​FluProbes 647H conjugation kit
  
• Rhodamine conjugation kit
• Texas Red^® conjugation kit

Fluorescent proteins and tandem dyes

• APC conjugation kit
• APC/Cy5.5^® conjugation kit
• APC/Cy7^® conjugation kit
• B-PE conjugation kit
• R-PE conjugation kit
• PE/Atto 594 conjugation kit
  
• PE/Cy5^® conjugation kit
• PE/Cy5.5^® conjugation kit
• PE/Cy7^®​ conjugation kit
• ​PE/Texas Red
  
• PerCP conjugation kit
• PerCP/Cy5.5^® conjugation kit

Biotin / Streptavidin

• Biotin conjugation kit (Type A - use with a streptavidin-labeled detection reagent)
• Biotin conjugation kit (Type B - use where the biotinylated protein is captured by streptavidin immobilized on a surface, eg, plates, nitrocellulose, magnetic beads etc)
• Streptavidin​ conjugation kit

Oligonucleotides

• Oligonucleotide conjugation kit

Gold

• Gold conjugation kit range for 10 nm, 20 nm, 40 nm, 60 nm and 80 nm gold

​​Metals

• Europium conjugation kit
  
• All Lanthanide metals

Particles / Beads

• Latex conjugation kits
• Magnetic bead conjugation kit

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Alexa Fluor® is a registered trademark of Life Technologies. Alexa Fluor® dye conjugation kits contain(s) technology licensed to Abcam by Life Technologies.