Antibody conjugation kits
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Quick & easy-to-use conjugation kits for over 45 labels.
Conjugating primary antibodies can lead to antibody loss and take up valuable lab time. Our innovative Lightning-Link® labeling technology enables the direct labeling of antibodies or proteins, providing a straightforward solution to traditional conjugation issues and delivering efficiency from discovery to development.
Overview
What are the benefits of Lightning-Link® antibody labeling technology?
- Simple & Consistent: Conjugate your antibody in three simple steps without the risk of antibody loss from a traditional, post-conjugation purification step
- Quick & Easy: Our conjugation kits only require 30 seconds of hands-on time so you can focus on your research. Watch our short protocol video to find out more
- Choice & Flexibility: Access the largest range of labels on the market to complete your experiment
- Scalable & Efficient: With kits that work with 10µg-100mg of antibody, achieve consistent conjugation whatever the scale of your experiment
How does Lightning-Link® work?
Simply pipette your antibody or biomolecule of choice into the vial of a lyophilized mixture containing the label of interest and incubate for just 15 minutes (Lightning-Link® Fast range) or around 3 hours (Lightning-Link® range).
Figure 1. Diagram illustrating the simple Lightning-Link® protocol
Despite its simple protocol, the Lightning-Link® process quickly generates reproducible conjugates with no loss of antibody, saving you valuable time and resources. It can also be used to conjugate other proteins or peptides. Learn more about the advantages of our antibody labeling kits.
What are the key considerations for antibody conjugation?
To achieve a successful conjugation, you should take these factors into account:
- Purification: only purified antibodies are suitable for conjugation (ie not crude serum or tissue culture supernatant)
- Buffer: certain buffer constituents are incompatible with the labeling reaction (see conjugation kit datasheet for details)
- Concentration: the antibody should be at least 1mg/mL concentration
If your antibody does not meet these requirements, you can easily purify or concentrate your antibody using our purification and concentration kits.
We have over 10,000 carrier-free antibodies specifically designed for antibody labeling. Carrier-free antibodies are conjugation-ready and there is no need to perform buffer exchange or concentration. Simply add the antibody directly into the conjugation reaction.
Why researchers love our conjugation kits
Lightning-Link® kits have been referenced by researchers in over 750 publications.
"The R-Phycoerythrin conjugation kit was extremely easy to use and effective despite the very small amount of antibody labeled (60µg). "
Researcher review for ab102918 R-PE conjugation kit"Improved staining for mouse-on-mouse immunofluorescence... Mouse Anti-CCX4 (ab27591) labeled with Dylight 550 Fast Conjugation Kit (ab201800)"
Researcher review for ab201800 Dylight 550 Conjugation Kit"The kit work[ed] fantastically with some store bought, pure, concentrated antibody! It actually gave better results than our previous method of using a conjugated secondary antibody when used in Flow Cytometry after doing an Intracellular Stain."
Researcher review for ab102884 FITC Conjugation Kit
Explore the full Lightning-Link® range to create your conjugate
Fluorescent dyes / proteins
Biotin / Streptavidin
Oligonucleotides