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Quick & easy-to-use conjugation kits for over 45 labels.
Conjugating primary antibodies can lead to a loss of antibody and take up valuable lab time. Our innovative Lighting-Link® labeling technology enables the direct labeling of antibodies or proteins, providing a straight-forward solution to traditional conjugation issues and deliver efficiency from discovery to development.
Simply pipette your antibody or biomolecule of choice into the vial of lyophilized mixture containing the label of interest and incubate for just 15 minutes (Lightning-Link® Fast range) or around 3 hours (Lightning-Link® range).
Figure 1. Diagram illustrating the simple Lightning-Link® protocol
Despite its simple protocol, the Lightning-Link® process quickly generates reproducible conjugates with no loss of antibody, saving you valuable time and resources. It can also be used to conjugate other proteins or peptides. You can learn about our antibody labeling kits and their advantages here.
To achieve a successful conjugation, you should take these factors into account:
If your antibody does not meet these requirements, you can easily purify or concentrate your antibody using our purification and concentration kits.
We have over 10,000 carrier-free antibodies specifically designed for antibody labeling. Carrier-free antibodies are conjugation-ready and there is no need to perform buffer exchange or concentration. Simply add the antibody directly into the conjugation reaction.
Lightning-Link® kits have been referenced by researchers in over 750 publications.
"The R-Phycoerythrin conjugation kit was extremely easy to use and effective despite the very small amount of antibody labeled (60µg). "
Researcher review for ab102918 R-PE conjugation kit
"Improved staining for mouse-on-mouse immunofluorescence... Mouse Anti-CCX4 (ab27591) labeled with Dylight 550 Fast Conjugation Kit (ab201800)"
Researcher review for ab201800 Dylight 550 Conjugation Kit
"The kit work[ed] fantastically with some store bought, pure, concentrated antibody! It actually gave better results than our previous method of using a conjugated secondary antibody when used in Flow Cytometry after doing an Intracellular Stain."
Researcher review for ab102884 FITC Conjugation Kit
Fluorescent dyes / proteins
Biotin / Streptavidin