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ATP assays are either based on the extraction of ATP from cells followed by light production with ATP-dependent luciferase, or the ATP-dependent phosphorylation of glycerol (or other substrates) to generate a detectable product.
No-wash assay. Same method as ATP assay. After ATP analysis, ADP is converted to ATP and detected.
Used with cell lysates.
Not as sensitive as luminescence assays.
“I personally recommend ab113849] to measure extracellular ATP levels for its ease of use and for the kindly attention and help I received from the Abcam scientific support specialists.”
- Dr. Lisa Sevenich
NADH, NAD, NADHP and NADP
NADP / NADPH