952 Metabolism purple

Assays for ATP, NADH, and associated molecules

Find your ideal assay

ATP assays are either based on the extraction of ATP from cells followed by light production with ATP-dependent luciferase, or the ATP-dependent phosphorylation of glycerol (or other substrates) to generate a detectable product.

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Assay

Read out

Notes

Assay kits

ATP

Plate reader

No-wash assay.

ab113849

ADP/ATP

No-wash assay. Same method as ATP assay. After ATP analysis, ADP is converted to ATP and detected.

ab65313

ATP

Used with cell lysates.

Not as sensitive as luminescence assays.

ab83355

Phosphate


ab65622, ab219938 ab102508

Pyrophosphate


ab112155 ab179836

​​​“I personally recommend ab113849] to measure extracellular ATP levels for its ease of use and for the kindly attention and help I received from the Abcam scientific support specialists.” 

- Dr. Lisa Sevenich

NADH, NAD, NADHP and NADP



Assay

Readout

Assay kits

NADH

Plate reader

ab186030

NAD/NADH

ab65348, ab176723, ab186032

NADP / NADPH

ab186033, ab65349, ab176724

NADPH

ab186031

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