CatchPoint® SimpleStep ELISA® Kits

​​

Don’t miss a thing with our fluorescent ELISA kits.

CatchPoint SimpleStep ELISA – an overview

CatchPoint SimpleStep ELISA kits have been developed using a fluorescent substrate to provide improved linearity over an extended dynamic range when compared to TMB substrate, the most widely used colorimetric substrate for ELISA. The extended dynamic range provides better quantification at both the lower and the upper end of the curve, as shown in the examples below.

• How to use CatchPoint SimpleStep ELISA kits
• CatchPoint SimpleStep ELISA example data

Additional benefits include:

• One-wash, 90-minute protocol: save precious time with a standardized and simplified protocol thanks to our innovative technology. Learn more about how our technology works here.
• A stable signal that provides readout flexibility: the signal is stable for up to 24 hours providing you with the flexibility to read the results at your convenience.
• No compromise on sensitivity: assay development is focused on achieving the highest sensitivity possible for each kit.
• Made with high-quality antibodies for batch-to-batch consistency: antibody production is controlled and reliable leading to improved consistency and reproducibility.

​

How to use CatchPoint SimpleStep ELISA kits

CatchPoint SimpleStep ELISA kits are optimized for Molecular Devices Multi-Mode Microplate Readers.

Take a look at the Molecular Devices application note for more information on how you can achieve improved performance of CatchPoint SimpleStep ELISA kits on Molecular Devices’ SpectraMax^® and FilterMax™ readers.

​

CatchPoint SimpleStep ELISA example data

The examples below show the improved linearity over an extended dynamic range of our Human ICAM-1 (ab229383) and Mouse FGF-21 (ab229382) CatchPoint SimpleStep ELISA kits in comparison to equivalent SimpleStep ELISA kits (ab174445 and ab212160) that use the TMB colorimetric substrate.

Figure 1.Standard curve comparison over extended dynamic range of Human ICAM-1 CatchPoint SimpleStep ELISA kit (ab229383) vs. Human ICAM-1 SimpleStep ELISA kit (ab174445). The fluorescent ELISA kit (ab229383) shows better linearity than the colorimetric ELISA kit (ab174445) over the extended dynamic range.

Figure 2. Signal to noise ratio over extended dynamic range of Human ICAM-1 CatchPoint SimpleStep ELISA kit (ab229383) vs. Human ICAM-1 SimpleStep ELISA kit (ab174445). In this example, the signal to noise ratio is used for normalization. The dotted lines mark the dynamic range recommended for the colorimetric ELISA kit (ab174445). The fluorescent ELISA kit (ab229383) shows better linearity over the extended dynamic range and increased signal to noise ratio.

Figure 3. Standard curve comparison over extended dynamic range of Mouse FGF-21 CatchPoint SimpleStep ELISA kit (ab229382) vs. Mouse FGF-21 SimpleStep ELISA kit (ab212160). The fluorescent ELISA kit (ab229382) shows better linearity than the colorimetric ELISA kit (ab212160) over the extended dynamic range.

Figure 4. Signal to noise ratio over extended dynamic range of Mouse FGF-21 CatchPoint SimpleStep ELISA kit (ab229382) vs. Mouse FGF-21 SimpleStep ELISA kit (ab212160). In this example, the signal to noise ratio is used for normalization. The dotted lines mark the dynamic range recommended for the colorimetric ELISA kit (ab212160). The fluorescent ELISA kit (ab229382) shows better linearity over the extended dynamic range and increased signal to noise ratio.