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The analysis of cell viability, cytotoxicity, cell cycle state, cell proliferation, and cell death is critical to most cell-based studies.
Understand your options, and choose the right assay to fit your experimental needs.
Or read this online version of the guide.
Learn more with our resources:
Cell viability assay guide
Including MTT, MTS, resazurin, and similar assays, mitochondrial membrane potential-dependent dyes, esterase cleaved dyes, ATP and ADP assays, and assays that measure glycolytic flux and oxygen consumption.
Cytotoxicity assay guide
Including LDH enzyme leakage assays, and membrane-impermeable dyes like 7-AAD.
Cell proliferation and cell cycle assay guide
Including assays using dye dilution, BrdU/EdU, and DNA staining dyes. These are used to monitor the growth of a cell population, detect generations of daughter cells, or analyze the cell cycle state of a cell population. You can use also these tests to assay cell viability.
Remember: no one method gives a perfect view of cell viability, proliferation, or cell death. It is almost always most effective to combine several different methods.