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Learn about your options when you need a cell viability assay.
Cell Counting Kit-8/CCK-8/ WST-8 assay ab228554: Plate reader.
XTT assay ab232856: Plate reader.
TMRE assay ab113852 / TMRM assay ab228569: TMRE is the most popular Abcam mitochondrial membrane dye assay. Ex/Em 549/575 nm. Washed out of mitochondria after fixation. Plate reader, microscope, flow cytometer.
JC-1 assay ab113850 / JC-10 assays ab112134 and ab112133: JC-1 (Ex/Em 530/530–570) and JC-10 (Ex/Em 590/520–570) form red aggregates at high concentrations (unaggregated dye is green). Loss of membrane potential causes loss of dye and increased green fluorescence. JC-10 is more soluble than JC-1. Washed out after fixation. Plate reader, microscope, flow cytometer.
Mitotracker Red assay: Ex/Em 579 /599. Not washed out after fixation. Plate reader, microscope, flow cytometer.
Rhodamine 123 assay: Ex/Em 507/529. Washed out after fixation. Plate reader, microscope, flow cytometer.
Calcein AM assay kit ab228556: Ex/Em 495/515 nm. Plate reader, microscope, flow cytometer.
Calcein violet AM ab176748: Ex/Em 405/460 nm. Plate reader, microscope, flow cytometer.
Esterase-cleaved blue assay ab112120: Ex/Em 360/450 nm. Plate reader.
Esterase-cleaved green assay ab112122: Ex/Em 490/520 nm. Plate reader, microscope.
Esterase-cleaved near IR assay ab112123: Ex/Em 633/660 nm. Plate reader, microscope.
Luminescence ATP assay ab113849: No-wash assay. Luminometric plate reader.
Luminescence ADP/ATP assay ab65313: No-wash assay. After ATP analysis, ADP is converted to ATP for detection. Luminometric plate reader.
Extracellular oxygen consumption assay ab197243: No-wash assay. Dye signal (Ex/Em 380/650 nm) increases as respiration lowers O2 levels. No need for specialized instruments. Plate reader.
Intracellular oxygen levels assay ab197245: Dye fluorescence (Ex/Em 340/642) is quenched by intracellular oxygen. No-wash assay. Plate reader.
Glycolysis activity assay ab197244: No-wash assay. Lactate production causes extracellular acidification and increased dye fluorescence (Ex/Em 340-380/615 nm). Plate reader.