Cellular metabolism assays

Test metabolism connections faster with easy-to-use optimized assay kits across a wide range of disease areas and metabolic events.

Contents

• What are metabolism assays?
• Benefits of our metabolism assays
• Find assays to key analytes within metabolic pathways
  • Glycolysis
  • Fatty acid metabolism 
  • Urea metabolism
  • Assays for ATP, NADH and other associated molecules

What are metabolism assays?

Metabolism is a complex process that lies at the core of biology. Metabolic changes can lead to a huge range of outcomes in different disease areas, from cancer or neurodegeneration to obesity and diabetes. Our assay kits enable you to analyze the pathways of metabolism in-depth in live cells, lysates, and biofluids with easy analysis on your plate reader. With 20 years of experience in manufacturing metabolism assay kits, we can help you understand your cell or disease model or how your target or compound affects your metabolic pathway/phenotype of interest.

Benefits of our metabolism assays:

• Easy-to-use with a simple step-by-step procedure included with each kit.
• Consistent: manufactured with strict QC criteria to ensure reproducible results and reliable long-term supply.
• Convenient: designed to deliver results with existing lab equipment, like plate readers, and with a range of sample types.
• Choice: a broad range of assays and reagents across the key analytes and targets within the most important metabolic pathways.

Find assays to key analytes within metabolic pathways

Glycolysis 

Glycolysis is the process by which glucose is converted into pyruvate to provide energy to cells. The glycolysis pathway is a sequence of reactions regulated by a few key enzymes including phosphofructokinase 1. Our assay kits allow you to measure the activity of these enzymes as well as the metabolites involved in this pathway.

4/5 Ease of Use – “We used this pyruvate assay to detect pyruvate in cell pellets and the kit was very simple to use and very fast.” 

5/5 Ease of Use –“Instructions were easy to follow with good results”. 

Researcher reviews for Pyruate Assay Kit (ab65342)

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Fatty acid metabolism

Fatty acid metabolism is key to the production and storage of energy. When released from adipocytes, fatty acid circulates as free fatty acid in the blood, before it enters cells and is broken down to produce energy. Our assay kits enable you to study both catabolic and anabolic fatty acid metabolism.

4/5 Ease of Use – “Awesome Experience. The kits were easy to use. You are going to [get] protocol with packages when they send to you.” 

5/5 Ease of Use – “This kit is easy to use and fast, and gives blood triglyceride concentrations in line with what is reported by literature. Our experience gives reproducible results across the different kit lots”.

Researcher review for Triglyceride Assay Kit – Quantification (ab65336)

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​Urea metabolism

The purpose of the urea cycle is to convert waste ammonia into urea for excretion, primarily taking place in the liver. Our assay kits all you to measure the activity of both the metabolites and enzymes involved in the urea cycle. 

5/5 Ease of Use – “Easy to follow instructions and good assay results.”

Researcher review for Urea Assay Kit (ab83362)

5/5 Ease of Use – “The assay protocol is good, the assay is easy and quick to perform.”

Researcher review for Glutamate Assay Kit (ab83389)

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Assays for ATP, NADH and other associated molecules

To further support your work across key metabolism pathways, we provide assay kits across a range of associated molecules. All cellular assay kits recommended below have a plate reader readout.