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ELISA standard curve
The standard or calibration curve is the element of the quantitative ELISA that will allow calculating the concentration of antigen in the sample.
The standard curve is derived from plotting known concentrations of a reference antigen against the readout obtained for each concentration (usually optical density at 450 nm).
Most ELISA plate readers will incorporate a software for curve fitting and data analysis. The concentration of the antigen in the sample is calculated by extrapolation of the linear portion of the standard curve.
Figure 1: Example of a quantitative ELISA standard curve from Human ICAM1 SimpleStep ELISA® Kit (ab174445).
Curve fitting software allow using different models to plot your data.