For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

ELISA kits

ELISA kits

Related

  • SimpleStep ELISA kits
    • In-Cell ELISA kit (ICE)
      • Immunoassay homepage

        Fully validated and ready-to-use enzyme-linked immunosorbent assay (ELISA) kits.

        Choose from our wide range of ELISA kits to detect or quantitate one or more specific proteins in a solution or biological sample.

        Figure 1. Different ELISA formats. 

        Different ELISA formats
        Find your ELISA kit


        Sandwich ELISA

        In a sandwich ELISA assay, an immobilized capture antibody pre-coated onto a 96-well plate binds to the target protein and captures it from a liquid sample.

        Detection antibodies are then added to create complexes where the detection antibodies also bind to the captured target protein, catalyze the appearance of a colored or fluorescent product through the action of a covalently linked enzyme.

        The amount of color produced is proportional to the amount of target protein in the sample. A sandwich ELISA can be:

        • Quantitative Insulin Human ELISA Kit SimpleStep (ab200011)
          • Measures the amount of Human insulin present in the sample
        • Semi-quantitative Cytochrome c Profiling ELISA Kit (ab110172) 
          • Detects Cytochrome C more sensitively than in a  Western Blot
        • Qualitative Anti-Chikungunya Virus IgG Human ELISA Kit (ab177835)
          • Where the color intensity is proportional to the amount of Chikungunya virus reactive IgG antibodies in the sample

        SimpleStep ELISA Kits

        Our brand of ELISA kits provide improved speed and performance while retaining the familiar process and standard data output of a traditional ELISA kit.

        SimpleStep ELISA kits reduce the number of wash steps from 9 (3x3) to 3 (1x3) by enabling complex formation in one step rather than sequentially. Total time required is less than two hours.

        We also offer ELISA sets with validated antibody pairs and standards for those wanting to develop their own ELISA method.

        Discover the benefits of SimpleStep ELISA kits


        Competitive ELISA

        In a competitive ELISA, sample antigen and labeled antigen compete for capture antibody binding. The more target protein there is in the sample, the less labeled antigen will be captured and the weaker the signal.

        • Corticosterone (CSCI) ELISA kit (ab108821)
          • Biotinylated corticosterone competes with corticosterone in the sample for binding to the capture antibody. The signal intensity is inversely proportional to the unlabeled corticosterone in the sample.

        Indirect ELISA

        In an indirect ELISA, the target protein adheres to the bottom of a 96-well plate, a primary antibody binds to the target, and a labeled secondary antibody binds to the primary for detection. The method can also be used to detect specific antibodies in a serum sample by substituting the serum for the primary antibody.

        The main advantage of an indirect ELISA is that one labeled secondary antibody can be used with many different primary antibodies.

        • Anti-Measles virus IgG Human ELISA kit (ab108750)
          • A plate pre-coated with Measles virus antigens binds measles antibodies from a serum sample.


        Example validation data
        ​

        Detection of frataxin in HeLa cells

        Figure 2. Detection of frataxin in HeLa cells with Frataxin ELISA kit (ab115346). Sensitivity = 0.3 ng/mL, intra-assay precision = 6% CV, inter-assay precision = 9.9% CV.

        Determination of the levels of frataxin in human samples

        Figure 3. Determination of the levels of frataxin in human samples. Transformed B lymphocyte cells from Friedreich's Ataxia samples are compared to heterozygous carrier B-lymphocyte cells and control B-lymphocyte cells. B lymphocyte extracts were analyzed at 0.2 mg/mL (10 µg/well) and frataxin concentrations were interpolated from the standard curve.

        Get resources and offers direct to your inbox Sign up
        A-Z by research area
        • Cancer
        • Cardiovascular
        • Cell biology
        • Developmental biology
        • Epigenetics & Nuclear signaling
        • Immunology
        • Metabolism
        • Microbiology
        • Neuroscience
        • Signal transduction
        • Stem cells
        A-Z by product type
        • Primary antibodies
        • Secondary antibodies
        • Biochemicals
        • Isotype controls
        • Flow cytometry multi-color selector
        • Kits
        • Loading controls
        • Lysates
        • Peptides
        • Proteins
        • Slides
        • Tags and cell markers
        • Tools & Reagents
        Help & support
        • Support
        • Make an Inquiry
        • Protocols & troubleshooting
        • Placing an order
        • RabMAb products
        • Biochemical product FAQs
        • Training
        • Browse by Target
        Company
        • Corporate site
        • Investor relations
        • Company news
        • Careers
        • About us
        • Blog
        Events
        • Tradeshows
        • Conferences
        International websites
        • abcam.cn
        • abcam.co.jp

        Join with us

        • LinkedIn
        • facebook
        • Twitter
        • YouTube
        • Terms of sale
        • Website terms of use
        • Cookie policy
        • Privacy policy
        • Legal
        • Modern slavery statement
        © 1998-2023 Abcam plc. All rights reserved.