ELISA kits
Related
Fully validated and ready-to-use enzyme-linked immunosorbent assay (ELISA) kits.
Choose from over 2,000 ELISA kits covering 1,000 specific targets in over 18 species including human, mouse and rat. From cytokines to popular cancer -related targets like PD-L1, from immunology to neuroscience, find the ELISA kit you need for your research.
All our ELISA kits are ready to use and we offer different formats: quantitative, semi-quantitative, indirect or competitive.
Our range include over 500 SimpleStep ELISA® kits for sensitive and consistent results in just 90 minutes.
Simplestep ELISA® kits use an original one incubation – one wash protocol, shortening the assay time to 90 minutes without any compromise on the assay performance. Made with recombinant rabbit monoclonal detection and capture antibodies, SimpleStep ELISA® kits offer excellent sensitivity and result consistency from one lot to another. All our SimpleStep ELISA kits follow the same standardised protocol for ease of use.
Our ELISA kits are fully validated to guarantee reliable results in various sample types:
| Product name (abID) | Human VEGF ELISA kit (ab222510) |
| Sample type | Saliva, Milk, Urine, Serum, Cell culture extracts, Heparin plasma, EDTA plasma, Citrate plasma |
| Sensitivity | 2.7 pg/ml |
| Dynamic range | 12.5 pg/ml – 800 pg/ml |
| Assay time | 1h 30min |
| Reactivity | Reacts with: Mouse, Rat, Dog, Human. Does not react with: Cow |
| Intra-assay precision | 5.4% |
| Inter-assay precision | 5.5% |
| Average recovery | 96% |
Figure 1. The VEGF standard curve was prepared as described in Section 10 of the protocol booklet. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Figure 2. The concentrations of VEGF were measured in duplicates, interpolated from the VEGF standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (heparin) 50% and plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VEGF concentration was determined to be 268.9 pg/mL in neat serum, 123.9 pg/mL in neat plasma (citrate), 133.8 pg/mL in neat plasma (heparin), and 95.76 pg/mL in neat plasma (EDTA).
Figure 3. Human PBMC (seeded at 10x106/mL) were cultured for 2 or 5 days in the presence or absence of 1.5% PHA-M. The concentrations of VEGF were measured in three different dilutions of the supernatant samples in duplicates and interpolated from the VEGF standard curve. The interpolated values are plotted (mean +/- SD, n=3). The mean VEGF concentration was determined to be 34.76 pg/mL in 2 Day PHA-M stimulated PBMC cell culture supernatant, 393.7 pg/mL in 5 Day PHA-M stimulated PBMC cell culture supernatant, and undetectable in unstimulated PBMC cell culture supernatant at both 2 and 5 days (not shown).
We also offer a range of validated paired monoclonal recombinant capture and detection antibodies complete with a calibrated protein standard allowing you to create your own sensitive ELISA assay.
Discover our matched antibody pairs