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Enzymatic double-staining IHC kits

Related

  • All IHC kits and reagents
    • Blocking reagents
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            • IHC-P protocol
              • IHC-Fr protocol

                Simplifying multicolor enzymatic IHC.

                DoubleStain IHC detection systems

                DoubleStain enzymatic IHC detection systems provide you with reagents and optimized protocols for staining two antigens on a tissue-section using immuno-enzymatic detection.

                • Rodent specific kits have been tested on paraffin-embedded mouse tissue
                • Human specific kits have been tested on paraffin embedded human tissue

                The kits also allow various combinations of primary antibodies and a choice enzymatic labels. Use the key below to identify the most suitable system.

                Multicolor enzymatic systems offer

                • Reduced background
                  • when staining tissue containing endogenous biotin (eg kidney, liver and spleen) using an HRP and AP polymer based system
                • Optimized protocol
                  • simultaneously incubate primary antibodies and subsequently simultaneously incubate enzyme conjugated secondary antibodies
                • Better signal to noise ratio
                  • primer used for clear visualization of staining and morphology
                • Color combinations
                  • red/green, red/purple or red/brown
                • Use of mouse antibodies on mouse tissue
                  • protocols for blocking endogenous mouse IgG
                • Combination with special stains where compatible


                Kit selection key:

                Primary antibody keyChromogen key
                G: Goat, M: Mouse, R: Rabbit, Rt: RatDAB: Brown, AP/Red & FastRed: Red, HRP/Green: Green, BCIP: Blue, AEC: Red

                *CL kits can be used in co-localization experiments

                Human tissue staining:

                Product nameSizeProduct code
                DoubleStain IHC kit: G&M on human tissue (DAB & AP/Red)12 mL, 36 mL & 120 mL

                ab183159

                DoubleStain IHC kit: G&M on human tissue (BCIP & AEC)12 mL, 36 mL & 120 mLab183271
                DoubleStain IHC kit: G&M on human tissue (HRP/Green & AP/Red)12 mL, 36 mL & 120 mLab183272
                DoubleStain IHC kit: M&R on human tissue (DAB & AP/Red)12 mL, 36 mL & 120 mLab210059
                DoubleStain IHC kit: M&R on human tissue (DAB & FastRed)12 mL, 36 mL & 120 mLab210062
                DoubleStain IHC kit: M&R on human tissue (BCIP & AEC)12 mL, 36 mL & 120 mLab210060
                DoubleStain IHC kit: M&R on human tissue (HRP/Green & FastRed)12 mL, 36 mL & 120 mLab210061

                Mouse and rat tissue staining

                Product nameSizeProduct code
                DoubleStain IHC kit: M&M on rodent tissue (DAB & AP/Red)12 mL, 36 mL & 120 mLab183273
                DoubleStain IHC kit: M&M on rodent tissue (BCIP & AEC)12 mL, 36 mL & 120 mLab183274
                DoubleStain CL IHC kit: M&M on rodent tissue (HRP/Green & AP/Red)12 mL, 36 mL & 120 mLab183275
                DoubleStain CL IHC kit: M&M on rodent tissue (DAB & FastRed)12 mL, 36 mL & 120 mLab183276
                DoubleStain IHC kit: M&Rt on mouse tissue (DAB & AP/Red)12 mL, 36 mL & 120 mLab183277
                DoubleStain IHC kit: M&Rt on mouse tissue (BCIP & AEC)12 mL, 36 mL & 120 mLab183278
                DoubleStain IHC kit: M&Rt on mouse tissue (HRP/Green & AP/Red)12 mL, 36 mL & 120 mLab183279

                Human and rodent tissue staining

                Product nameSizeProduct code
                DoubleStain IHC kit: G&Rt on human/mouse tissue (DAB & AP/Red)12 mL, 36 mL & 120 mLab183280
                DoubleStain IHC kit: G&Rt on human/mouse tissue (BCIP & AEC)12 mL, 36 mL & 120 mLab183281
                DoubleStain CL IHC kit: G&Rt on human/mouse tissue (HRP/Green & AP/Red)12 mL, 36 mL & 120 mLab183282
                DoubleStain IHC kit: R&Rt on human/mouse tissue (DAB & AP/Red)12 mL, 36 mL & 120 mLab183283
                DoubleStain IHC kit: R&Rt on human/mouse tissue (BCIP & AEC)12 mL, 36 mL & 120 mLab183284
                DoubleStain IHC kit: R&Rt on human/mouse tissue (HRP/Green & AP/Red)12 mL, 36 mL & 120 mLab183285

                Applications

                Our double-staining IHC kits can enable you to perform multicolor IHC experiments where you can

                • Stain different cell types to get an overview of their location in tissue specimens
                • Identify cells using a structural marker as well as an antibody against your target protein
                • Search for co-localization when combining cellular markers with activation markers
                • Perform histology experiments using visible color with no need to use a fluorescence microscope
                • Combine with fluorescent in-situ hybridization markers to simultaneously study protein and mRNA protein levels
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