Enzymatic double-staining IHC kits
Related
Simplifying multicolor enzymatic IHC.
DoubleStain IHC detection systems
DoubleStain enzymatic IHC detection systems provide you with reagents and optimized protocols for staining two antigens on a tissue-section using immuno-enzymatic detection.
- Rodent specific kits have been tested on paraffin-embedded mouse tissue
- Human specific kits have been tested on paraffin embedded human tissue
The kits also allow various combinations of primary antibodies and a choice enzymatic labels. Use the key below to identify the most suitable system.
Multicolor enzymatic systems offer
- Reduced background
- when staining tissue containing endogenous biotin (eg kidney, liver and spleen) using an HRP and AP polymer based system
- Optimized protocol
- simultaneously incubate primary antibodies and subsequently simultaneously incubate enzyme conjugated secondary antibodies
- Better signal to noise ratio
- primer used for clear visualization of staining and morphology
- Color combinations
- red/green, red/purple or red/brown
- Use of mouse antibodies on mouse tissue
- protocols for blocking endogenous mouse IgG
- Combination with special stains where compatible
Kit selection key:
| Primary antibody key | Chromogen key |
| G: Goat, M: Mouse, R: Rabbit, Rt: Rat | DAB: Brown, AP/Red & FastRed: Red, HRP/Green: Green, BCIP: Blue, AEC: Red |
*CL kits can be used in co-localization experiments
Human tissue staining:
| Product name | Size | Product code |
| DoubleStain IHC kit: G&M on human tissue (DAB & AP/Red) | 12 mL, 36 mL & 120 mL | |
| DoubleStain IHC kit: G&M on human tissue (BCIP & AEC) | 12 mL, 36 mL & 120 mL | ab183271 |
| DoubleStain IHC kit: G&M on human tissue (HRP/Green & AP/Red) | 12 mL, 36 mL & 120 mL | ab183272 |
| DoubleStain IHC kit: M&R on human tissue (DAB & AP/Red) | 12 mL, 36 mL & 120 mL | ab210059 |
| DoubleStain IHC kit: M&R on human tissue (DAB & FastRed) | 12 mL, 36 mL & 120 mL | ab210062 |
| DoubleStain IHC kit: M&R on human tissue (BCIP & AEC) | 12 mL, 36 mL & 120 mL | ab210060 |
| DoubleStain IHC kit: M&R on human tissue (HRP/Green & FastRed) | 12 mL, 36 mL & 120 mL | ab210061 |
Mouse and rat tissue staining
| Product name | Size | Product code |
| DoubleStain IHC kit: M&M on rodent tissue (DAB & AP/Red) | 12 mL, 36 mL & 120 mL | ab183273 |
| DoubleStain IHC kit: M&M on rodent tissue (BCIP & AEC) | 12 mL, 36 mL & 120 mL | ab183274 |
| DoubleStain CL IHC kit: M&M on rodent tissue (HRP/Green & AP/Red) | 12 mL, 36 mL & 120 mL | ab183275 |
| DoubleStain CL IHC kit: M&M on rodent tissue (DAB & FastRed) | 12 mL, 36 mL & 120 mL | ab183276 |
| DoubleStain IHC kit: M&Rt on mouse tissue (DAB & AP/Red) | 12 mL, 36 mL & 120 mL | ab183277 |
| DoubleStain IHC kit: M&Rt on mouse tissue (BCIP & AEC) | 12 mL, 36 mL & 120 mL | ab183278 |
| DoubleStain IHC kit: M&Rt on mouse tissue (HRP/Green & AP/Red) | 12 mL, 36 mL & 120 mL | ab183279 |
Human and rodent tissue staining
| Product name | Size | Product code |
| DoubleStain IHC kit: G&Rt on human/mouse tissue (DAB & AP/Red) | 12 mL, 36 mL & 120 mL | ab183280 |
| DoubleStain IHC kit: G&Rt on human/mouse tissue (BCIP & AEC) | 12 mL, 36 mL & 120 mL | ab183281 |
| DoubleStain CL IHC kit: G&Rt on human/mouse tissue (HRP/Green & AP/Red) | 12 mL, 36 mL & 120 mL | ab183282 |
| DoubleStain IHC kit: R&Rt on human/mouse tissue (DAB & AP/Red) | 12 mL, 36 mL & 120 mL | ab183283 |
| DoubleStain IHC kit: R&Rt on human/mouse tissue (BCIP & AEC) | 12 mL, 36 mL & 120 mL | ab183284 |
| DoubleStain IHC kit: R&Rt on human/mouse tissue (HRP/Green & AP/Red) | 12 mL, 36 mL & 120 mL | ab183285 |
Applications
Our double-staining IHC kits can enable you to perform multicolor IHC experiments where you can
- Stain different cell types to get an overview of their location in tissue specimens
- Identify cells using a structural marker as well as an antibody against your target protein
- Search for co-localization when combining cellular markers with activation markers
- Perform histology experiments using visible color with no need to use a fluorescence microscope
- Combine with fluorescent in-situ hybridization markers to simultaneously study protein and mRNA protein levels