Enzymatic double-staining IHC kits

Simplifying multicolor enzymatic IHC

DoubleStain IHC Detection Systems

DoubleStain enzymatic IHC detection systems provide you with reagents and optimized protocols for staining two antigens on a tissue-section using immuno-enzymatic detection.

  • Rodent specific kits have been tested on paraffin embedded mouse tissue
  • Human specific kits have been tested on paraffin embedded human tissue

The kits also allow various combinations of primary antibodies and a choice enzymatic labels (please use the key below to identify the most suitable system).

Multicolor enzymatic systems offer:

  • Reduced background
    • when staining tissue containing endogenous biotin (e.g. kidney, liver and spleen) using an HRP and AP polymer based system
  • Optimized protocol
    • simultaneously incubate primary antibodies and subsequently simultaneously incubate enzyme conjugated secondary antibodies
  • Better signal to noise ratio
    • primer used for clear visualization of staining and morphology
  • Color combinations
    • red/green, red/purple or red/brown
  • Use of mouse antibodies on mouse tissue
    • protocols for blocking endogenous mouse IgG
  • Combination with special stains where compatible


Kit selection key:

Primary antibody keyChromogen key
G: Goat, M: Mouse, R: Rabbit, Rt: RatDAB: Brown, AP/Red & FastRed: Red, HRP/Green: Green, BCIP: Blue, AEC: Red

*CL kits can be used in co-localization experiments

Human Tissue Staining:

Product nameSizeProduct code
DoubleStain IHC kit: G&M on human tissue (DAB & AP/Red)12 mL, 36 mL & 120 mL

ab183159

DoubleStain IHC kit: G&M on human tissue (BCIP & AEC)12 mL, 36 mL & 120 mL
ab183271
DoubleStain IHC kit: G&M on human tissue (HRP/Green & AP/Red)12 mL, 36 mL & 120 mL
ab183272
DoubleStain IHC kit: M&R on human tissue (DAB & AP/Red)12 mL, 36 mL & 120 mL
ab210059
DoubleStain IHC kit: M&R on human tissue (DAB & FastRed)12 mL, 36 mL & 120 mL
ab210062
DoubleStain IHC kit: M&R on human tissue (BCIP & AEC)12 mL, 36 mL & 120 mL
ab210060
DoubleStain IHC kit: M&R on human tissue (HRP/Green & FastRed)12 mL, 36 mL & 120 mL
ab210061

Mouse & Rat Tissue Staining:

Product nameSizeProduct code
DoubleStain IHC kit: M&M on rodent tissue (DAB & AP/Red)12 mL, 36 mL & 120 mL
ab183273
DoubleStain IHC kit: M&M on rodent tissue (BCIP & AEC)12 mL, 36 mL & 120 mL
ab183274
DoubleStain CL IHC kit: M&M on rodent tissue (HRP/Green & AP/Red)12 mL, 36 mL & 120 mL
ab183275
DoubleStain CL IHC kit: M&M on rodent tissue (DAB & FastRed)12 mL, 36 mL & 120 mL
ab183276
DoubleStain IHC kit: M&Rt on mouse tissue (DAB & AP/Red)
12 mL, 36 mL & 120 mL
ab183277
DoubleStain IHC kit: M&Rt on mouse tissue (BCIP & AEC)
12 mL, 36 mL & 120 mL
ab183278
DoubleStain IHC kit: M&Rt on mouse tissue (HRP/Green & AP/Red)
12 mL, 36 mL & 120 mL
ab183279

Human and Rodent Tissue Staining:

Product nameSizeProduct code
DoubleStain IHC kit: G&Rt on human/mouse tissue (DAB & AP/Red)12 mL, 36 mL & 120 mL
ab183280
DoubleStain IHC kit: G&Rt on human/mouse tissue (BCIP & AEC)12 mL, 36 mL & 120 mL
ab183281
DoubleStain CL IHC kit: G&Rt on human/mouse tissue (HRP/Green & AP/Red)12 mL, 36 mL & 120 mL
ab183282
DoubleStain IHC kit: R&Rt on human/mouse tissue (DAB & AP/Red)12 mL, 36 mL & 120 mL
ab183283
DoubleStain IHC kit: R&Rt on human/mouse tissue (BCIP & AEC)12 mL, 36 mL & 120 mL
ab183284
DoubleStain IHC kit: R&Rt on human/mouse tissue (HRP/Green & AP/Red)12 mL, 36 mL & 120 mL
ab183285

Applications

Our double-staining IHC kits can enable you to perform multicolor IHC experiments where you can:

  • Stain different cell types to get an overview of their location in tissue specimens
  • Identify cells using a structural marker as well as an antibody against your target protein
  • Search for co-localization when combining cellular markers with activation markers
  • Perform histology experiments using visible color with no need to use a fluorescence microscope
  • Combine with fluorescent in-situ hybridization markers to simultaneously study protein and mRNA protein levels