Enzymatic triple-staining IHC kits

Simplifying multicolor enzymatic IHC

TripleStain IHC Detection Systems

TripleStain enzymatic IHC detection systems provide you with reagents and optimized protocols for staining three antigens on a tissue-section using immuno-enzymatic detection.

  • Animal specific kits have been tested of paraffin embedded mouse tissue
  • Human specific kits have been tested on paraffin embedded human tissue

The kits also allow various combinations of species primary antibodies and a choice enzymatic labels (please use the key below to identify the most suitable system).

These multicolor enzymatic systems offer:

  • Reduced background
    • when staining tissue containing endogenous biotin (e.g. kidney, liver  & spleen) using an HRP and AP polymer based system
  • Optimized protocols
    • staining in five hours excluding antigen retrieval steps
  • Various color combinations
    • brown, red, green, brown & black depending on kit
  • Can be permanently mounted with a coverslip
  • Use  of mouse antibodies on mouse tissue
    • protocols for blocking endogenous mouse IgG

Example staining

Hu-Colon-Cancer-RxMxM

Staining of FFPE Human colon cancer tissue section using a Rabbit antibody against CEA (brown), a Mouse antibody against PCNA (black) and a Mouse antibody against smooth muscle actin (red) using  TripleStain IHC kit: M&M&R on human tissue (DAB, AP/Red & DAB/Ni).

Kit selection key:

Primary antibody keyChromogen key
G: Goat, M: Mouse, R: Rabbit, Rt: RatDAB: Brown, AP/Red & FastRed: Red, HRP/Green: Green, DAB/Ni: Black

Size guidelines if using 100µl polymer conjugate per slide : 24ml (~120 slides), 72ml (~360 slides), 240ml (1200 slides).

Human Tissue Staining:

Product nameSizeProduct code
TripleStain IHC kit: M&M&R on human tissue (DAB, AP/Red & HRP/Green)24ml, 72ml, 240mlab183286
TripleStain IHC kit: M&M&R on human tissue (DAB, AP/Red & DAB/Ni)24ml, 72ml, 240mlab183287
TripleStain IHC kit: R&R&M on human tissue (DAB, AP/Red & HRP/Green)24ml, 72ml, 240mlab183288
TripleStain IHC kit: R&R&M on human tissue (DAB, AP/Red & DAB/Ni)24ml, 72ml, 240mlab183289
TripleStain IHC kit: M&R&G on human tissue (DAB, AP/Red & HRP/Green)24ml, 72ml, 240mlab183290
TripleStain IHC kit: M&R&G on human tissue (DAB, AP/Red & DAB/Ni)24ml, 72ml, 240mlab183291

Rodent Tissue Staining:

Product nameSizeProduct code
TripleStain IHC kit: M&M&R on rodent tissue (DAB, HRP/Green & AP/Red)24ml, 72ml, 240mlab183292
TripleStain IHC kit: M&M&R on rodent tissue (DAB, AP/Red & DAB/Ni)24ml, 72ml, 240mlab183294
TripleStain IHC kit: R&R&M on rodent tissue (DAB , AP/Red & HRP/Green)24ml, 72ml, 240mlab183295
TripleStain IHC kit: R&R&M on rodent tissue (DAB, AP/Red & DAB/Ni)24ml, 72ml, 240mlab183296
TripleStain IHC kit: M&R&Rt on rodent tissue (DAB, HRP/Green & AP/Red)24ml, 72ml, 240mlab183297
TripleStain IHC kit: M&R&Rt on rodent tissue (DAB, DAB/Ni & AP/Red)24ml, 72ml, 240mlab183298

Applications

Our double-staining IHC kits can enable you to perform multicolor IHC experiments where you can:

  • Stain different cell types to get an overview of their location in tissue specimens
  • Identify cells using a structural marker as well as an antibody against your target protein
  • Search for co-localization when combining cellular markers with activation markers
  • Perform histology experiments using visible color with no need to use a fluorescence microscope
  • Combine with fluorescent in-situ hybridization markers to simultaneously study protein and mRNA protein levels
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