FirePlex®-96 immunoassays
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Multiplex quantitation of protein biomarkers with high-performance antibody pairs.
FirePlex-96 immunoassays feature fully customizable multiplex panels that allow you to analyze up to 70 analytes simultaneously per well, with the fast and easy readout on standard flow cytometers. Providing comprehensive biomarker profiling, directly from biofluids, FirePlex immunoassays are a fast, easy way to accelerate and scale up your research while minimizing your workload.
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Advantages of Fireplex®-96 immunoassays
FirePlex immunoassays combine innovative FirePlex particle technology along with Abcam’s optimized matched antibody pairs for analyte detection, offering the highest standards in sensitivity and specificity including:
- Analysis of up to 70 analytes per well
- 0.5 pg/mL to 30,000 pg/mL detection
- Up to 5-log dynamic range
- Only 12.5 µL of sample input, direct from biofluids
Browse our FirePlex®-96 immunoassay product range
Mix and match analytes from existing panels in our assay designer
Complex data analysis is made simple with free-of-charge integrated FirePlex Analysis Workbench software, which automatically interprets results and generates publication-ready figures. Samples can be analyzed on your own flow cytometer or sent for analysis in our service lab. If you leave the work to us, our full-service team of experts will analyze your samples from start to finish.
Download our FirePlex®-96 immunoassay technical note to learn more.
Compatibility with flow cytometers
FirePlex immunoassays can be conveniently run on your existing flow cytometer, eliminating the need to purchase dedicated equipment. FirePlex particles are validated for use with flow cytometers fitted with green, yellow, and red detectors. The following excitation and emission settings are required:
Compatibility with flow cytometers
FirePlex immunoassays can be conveniently run on your existing flow cytometer, eliminating the need to purchase dedicated equipment. FirePlex particles are validated for use with flow cytometers fitted with green, yellow, and red detectors. The following excitation and emission settings are required:
Our Cytometer Setup kit (ab245835) can be used to ensure a cytometer is compatible and determine the appropriate settings without having to use precious samples. The cytometer set up kit contains FirePlex particles that are run on the cytometer to establish configuration settings and ascertain which run buffer is most suitable in our software.
To ensure optimal performance of the FirePlex Immunoassay, we recommend that the instrument used has the sample loader fitted.
Various cytometers can be compatible with our FirePlex particle technology, including:
- Millipore's Guava EasyCyte™
- BD Biosciences' Accuri™ C6
If you’re interested in testing FirePlex technology on a different instrument or require technical support, please contact us.
FirePlex-96 immunoassay panels
Pre-designed FirePlex-96 panels are ideal for biomarker discovery projects as well as validation studies.
Human analytes and panels
Comprehensively profile up to 70 biomarker candidates simultaneously, directly from human plasma (EDTA, heparin, citrate), serum, urine, saliva, milk (defatted), synovial fluid, bronchoalveolar lavage (BAL), cerebrospinal fluid (CSF), or cell culture supernatant. FirePlex human immunoassays give you the flexibility, speed, and high-performance to take biomarker studies from the bench to the bedside.
Check out our pre-designed human panels:
| Human panels: | ||
| Discovery | Please see the flyer for the full list of 70 analytes | ab243551 |
| Inflammation | IL-1β, IL-6, IL-8, IL-10, IL-12p70, IFN-γ, MCP1, TNF-α | ab243550 |
| Key cytokine | IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-9, IL-10, IL-12p70, IL-13, IL-17A, GM-CSF, IFN-γ, MCP1, MIP1-α, MIP1-β, TNF-α | ab243549 |
Rodent analytes and panels
FirePlex mouse and rat immunoassay product lines provide essential tools for preclinical drug development research, whether identifying disease biomarkers, validating in vitro studies, or screening drug candidates for toxicity.
Allowing a multiplex analysis of up to 70 analytes per well, directly from plasma (EDTA, heparin, citrate), serum, urine, or cell culture supernatant, our specialized mouse panels and analytes offer fast, easy, and high-performance biomarker analysis to streamline your research pipeline.
| Mouse panels: | ||
| Discovery | Please see the flyer for the full list of analytes | ab243554 |
| Key cytokine | CXCL1, GM-CSF, IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p70, IL-13, IL-17A, MCP1, MIP1α, MIP1β, TNFα | ab235656 |
| Inflammation | CXCL1, IFNγ, IL-1β, IL-6, IL-10, IL-12p70, MCP1, TNFα | ab235659 |
| Kidney toxicity | Clusterin, Cystatin C, KIM-1, Lipocalin-2, Osteopontin | ab235661 |
| Rat panel: | ||
Key cytokine | CXCL1, GM-CSF, IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p70, IL-13, IL-17A, MCP1, MIP1α, MIP1β, TNFα | ab235662 |
| Key cytokine | GM-CSF, IFNγ, IL-1β, IL-10, IL-12p70, IL-13, IL-17A, IL-2, IL-4, IL-5, IL-6, IL-8, MCP-1, MIP1α, MIP1β, TNFα | ab239445 |
For tissue lysate preparation, we have a FirePlex intracellular immunoassay core kit (ab239454).
Design your ideal immunoassay panel using Mix and Match assay designer
You can mix and match analytes from existing panels in our assay designer. Choose from human or mouse and select up to 35 analytes to build your perfect panel.
Pick your perfect cytokine immunoassay panel from ever-expanding list of antibody pairs
When you need to quantify a specific set of cytokines, the choices for selecting a multiplex panel are often inflexible or inconvenient. Abcam’s FirePlex multiplex immunoassays use our unique FirePlex® particle technology to enable fully customizable panels for cytokine analysis. You can select the exact analytes for your panel, all your analytes are analyzed in one well, without the need to split your sample across multiple wells or panels.
Send us your samples and take advantage of our fully flexible panels through our cytokine profiling service; or run the assay and analysis in your lab.
Designed and tested with multiplexing in mind
In addition to using the unique properties of FirePlex® particles to support fully flexible multiplex panels, our FirePlex assays are also carefully tested for analyte multiplex interference and assay compatibility. This means that almost all our tested assays can be assembled into multiplex panels freely and without restriction.
If you're interested in FirePlex immunoassays, please get in touch with us.
Publications featuring FirePlex-96 immunoassays
Researchers around the globe are forging new biomedical frontiers with FirePlex immunoassays. From biotechnology to clinical care, FirePlex profiling technology provides the analytical power to ask new questions, drive new avenues of research, and provide the answers that drive medicine forward.
The superior performance of FirePlex assays and their ability to push the bounds of biomedical research is reflected in the publication record and representation in the highest impact journals. Below are some of the publications featuring FirePlex standard immunoassays.
Check out selected publications below:
- Laval K, Vernejoul JB, Van Cleemput J, Koyuncu OO, Enquist LW.(2018) Virulent PRV infection induces a specific and lethal systemic inflammatory response in mice. JVI.01614-18.
- LeFrancais E et al. (2018) Maladaptive role of neutrophil extracellular traps in pathogen-induced lung injury. JCI Insight. 3(3).
- Wang L et al. (2018) Inflammatory cytokine expression in patients with sepsis at an intensive care unit. Exp Ther Med. 16(212).
- Yánez DC et al. (2018) IFITM proteins drive type 2 T helper cell differentiation and exacerbate allergic airway inflammation. Eur J Immunol.
- FirePlex®-96 immunoassay technical note
- FirePlex® assays for rapid results in SARS-CoV-2 research leaflet
FirePlex® is a registered trademark in certain territories (including Europe and the United States) and is an unregistered trademark elsewhere.