High-throughput FirePlex Immunoassays

High-throughput features, without compromising high-performance multiplex analysis.

FirePlex high-throughput immunoassays, FirePlex-384 and FirePlex-1536 offer the high standards of performance and multiplexing you expect from our original FirePlex immunoassays, now in a high-throughput, automation-friendly format.

Ideal for discovery, screening, and profiling projects, high-throughput Fireplex immunoassays offers you a fast, easy, and cost-effective tool to analyze thousands of samples rapidly. Reach your goals faster, accelerate your path to success with FirePlex immunoassays.

NEW automation-friendly assays to accelerate your immunoassay workflow:

384-well and 1536-well plate assay format
Simple, no-wash, two-step (384-well) ore one-step (1586-well) workflow
Readout on high-content imager, < 20 min scan/384-well plate, about 1 hour/1536-well plate
Analyze up to 10 analytes per well in the 384-well and up to 5 analytes per well in the 1536-well assay

Accelerate your research
Assay workflow
Drug development applications
Scale-up without sacrificing performance
Flexibility in analytes

Accelerate your research

FirePlex immunoassays use the FirePlex particle technology and our validated antibody pairs to deliver rapid, flexible, robust, and sensitive high-throughput multiplex screening without the need for dedicated instrumentation. Using your high content imager, you can analyze up to 10 analytes per well with FirePlex-384 and up to 6 analytes per well with FirePlex-1536, with the whole process taking less than 20 minutes per plate.

Learn more about FirePlex-384 or FirePlex-1536 immunoassays.

The assay workflow

Figure 1. The assay workflow. To capture analytes onto FirePlex particles, biological samples are added to a 384-well imaging plate and incubated with the FirePlex-384 Reagent Mix overnight. Subsequently, an Imaging Dye is added, and plates are scanned on high-content imagers. The FirePlex Analysis Workbench software generates standard curves and quantifies analytes of interest directly from image files.

Drug development applications

High throughput FirePlex immunoassays can be applied at all stages of drug development to inform critical decision points. By providing a new, more effective, and affordable biomarker analysis workflow, FirePlex immunoassays enable data-driven R&D to guide the most effective strategies and minimize the risk of late-stage failure.

Discovery projects screening large compound libraries
Lead optimization, safety assessment, and toxicity screening in large sample sets
Clinical screening for disease identification, patient stratification, or therapy response in large patient cohorts

Scale-up without sacrificing performance

With high-throughput FirePlex immunoassays, there is no trade-off between quantity and quality. Achieve high throughput without sacrificing assay performance or data quality.

1-100 pg/mL sensitivity (analyte dependent)
2.5-3 logs dynamic range
Only 6.5 µL biofluid input for 384-well plate (plasma, serum, or cell culture supernatant); 2.5 µL biofluid input for 1536-well plate (Cell culture supernatant)
<15% intra-plate CVs
R ecombinant antibody pairs eliminate lot-to-lot variability
70-130% sample recovery
Reread plates up to a week later, as many times as needed