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Accelerate immunoassay preparation in a no-wash, automated workflow in 384- and 1536-well high-throughput formats. Our class-leading FirePlex multiplex assays with outstanding reproducibility and specificity allow you to achieve high throughput without sacrificing assay performance or data quality.
Reviewed October 22, 2021
See how Fireplex-384 and Fireplex-1536 immunoassays help advance immunoassay workflow:
Class-leading high-throughput formats
Simple, no-wash workflow
Robust, reproducible data with each assay
Quick and easy scale-up with automated workflow: readout on high-content imagers
< 20 min
Ideal for discovery, screening, and profiling projects, high-throughput FirePlex immunoassays offer a fast, easy, and cost-effective tool to analyze thousands of samples in half the time. Reach R&D goals faster by harvesting more insightful data with FirePlex-384 and FirePlex-1536 immunoassays.
Find out more about the benefits of choosing FirePlex immunoassays from Dr Paul Wylie.
FirePlex immunoassays use the FirePlex particle technology and validated antibody pairs to deliver rapid, robust, and sensitive high-throughput multiplex screening without the need for dedicated instrumentation. Using a high content imager, you can analyze up to:
Figure 1. The assay workflow. To capture analytes onto FirePlex particles, biological samples are added to a 384-well imaging plate and incubated with the FirePlex-384 Reagent Mix overnight. Subsequently, an Imaging Dye is added, and plates are scanned on high-content imagers. The FirePlex Analysis Workbench software generates standard curves and quantifies analytes of interest directly from image files.
To learn more about the FirePlex immunoassay platform, download FirePlex high-throughput poster
High-throughput FirePlex immunoassays can be applied at all stages of drug development to inform critical decision points:
By providing a new, more effective biomarker analysis workflow (Figure1), FirePlex immunoassays enable data-driven R&D to guide the most effective strategies and minimize the risk of late-stage failures.
Our FirePlex immunoassays offer high reproducibility and sensitivity, so you can achieve high throughput without sacrificing assay performance or data quality.
FirePlex-384 and FirePlex-1536 assays are compatible with high content imagers from various prominent manufacturers, including Molecular Devices, Perkin Elmer, GE, Yokogawa, Agilent, and Thermo. Check the required characteristics in Figure 2 below.
Figure 2. A. Varying intensities of green and yellow fluorescent dyes are used for particle identity barcoding; yellow dye, which is co-locally separate on the particle from the barcoding region, is used for analyte quantitation. B. Two images are captured per well, with approximately 20 particles analyzed per analyte using the FirePlex Analysis Workbench software. C. Representative graph demonstrates analyte fluorescent intensity levels automatically output from scanned wells along with analyte standard curves. D. List of required specifications for high-content imagers.
FirePlex Imager Setup Kit (ab229130) will help facilitate instrument set-up by identifying a compatible high content imager.
Interested in FirePlex high-throughput immunoassays? Fill out an online inquiry form.
Watch below D. Paul Wylie discuss how researchers profiled secreted proteins from iPSCs using the FirePlex-384 immunoassay or download a printable case study here.
FirePlexis a registered trademark in certain territories (including Europe and the United States) and is an unregistered trademark elsewhere.