High-throughput FirePlex® multiplex assays: Power your pipeline with more insightful data

Accelerate immunoassay preparation in a no-wash, automated workflow in 384- and 1536-well high-throughput formats. Our class-leading FirePlex multiplex assays with outstanding reproducibility and specificity allow you to achieve high throughput without sacrificing assay performance or data quality. 

Get in touch with us about FirePlex multiplex assays.

Contents

• Introducing high-throughput FirePlex immunoassays

• Advantages of high-throughput FirePlex-384 and FirePlex-1536 immunoassays

  • Accelerate next discovery with a fast, automated workflow

  • Gather more insightful data for drug development applications

  • Scale-up without sacrificing performance

  • Be flexible with analytes

• Simple data collection on high content imagers

• Choose the suitable high-throughput FirePlex assay format

  • FirePlex-384 standard panels

  • Mix and Match panels

  • Custom panel options

• Case study: Profiling of secreted proteins from iPSCs with FirePlex-384

• Recent publications featuring FirePlex-384 immunoassays

Introducing high-throughput FirePlex immunoassays

See how Fireplex-384 and Fireplex-1536 immunoassays help advance immunoassay workflow: 

 Hear more from Dr Paul Wylie, Director of Multiplex, Kits and Assay at Abcam, as he introduces FirePlex-384 and FirePlex-1536 assays. 

The advantages of high-throughput FirePlex-384 and FirePlex-1536 immunoassays

Ideal for discovery, screening, and profiling projects, high-throughput FirePlex immunoassays offer a fast, easy, and cost-effective tool to analyze thousands of samples in half the time. Reach R&D goals faster by harvesting more insightful data with FirePlex-384 and FirePlex-1536 immunoassays.

Find out more about the benefits of choosing FirePlex immunoassays from Dr Paul Wylie.

​Accelerate next discovery with a fast, automated workflow

FirePlex immunoassays use the FirePlex particle technology and validated antibody pairs to deliver rapid, robust, and sensitive high-throughput multiplex screening without the need for dedicated instrumentation. Using a high content imager, you can analyze up to:

• 10 analytes/well with FirePlex-384, < 20 min scan per plate
• 5 analytes/well with FirePlex-1536, < 1 hour scan per plate

FirePlex-384 and ​​FirePlex-1536 are ideally suited for high-throughput automated workflows (Figure 1). You can use any compatible liquid handling robot for buffer and sample dispensing into wells to simplify preparation and eliminate the ergonomic load on lab personnel caused by repeat pipetting.

Figure 1. The assay workflow. To capture analytes onto FirePlex particles, biological samples are added to a 384-well imaging plate and incubated with the FirePlex-384 Reagent Mix overnight. Subsequently, an Imaging Dye is added, and plates are scanned on high-content imagers. The FirePlex Analysis Workbench software generates standard curves and quantifies analytes of interest directly from image files.

To learn more about the FirePlex immunoassay platform, download FirePlex high-throughput poster.​

​Gather more insightful data for drug development applications

High-throughput FirePlex immunoassays can be applied at all stages of drug development to inform critical decision points:

• Discovery projects screening large compound libraries
• Lead optimization, safety assessment, and toxicity screening in large sample sets
• Clinical screening for disease identification, patient stratification, or therapy response in large patient cohorts

By providing a new, more effective biomarker analysis workflow (Figure1), FirePlex immunoassays enable data-driven R&D to guide the most effective strategies and minimize the risk of late-stage failures. ​

Scale-up without sacrificing performance

Our FirePlex immunoassays offer high reproducibility and sensitivity, so you can achieve high throughput without sacrificing assay performance or data quality.

• 1-100 pg/mL sensitivity (analyte dependent)
• 2.5-3 logs dynamic range
• Only 6.5 µL biofluid input for 384-well plate (plasma, serum, or cell culture supernatant); 2.5 µL biofluid input for 1536-well plate (Cell culture supernatant)
• <15% intra-plate CVs
• The use of stringent production and QC processes ensures lot-to-lot consistency
• 70-130% sample recovery
• Re-read plates up to a week later, as many times as needed

​Flexibility in analytes

Choose from: 

• Pre-designed standard panels, Mix and Match panels, or custom panel options
• Top 50 key cytokines and subsets of critical organ toxicity markers 
• Human and mouse analytes

Simple data collection on high content imagers

FirePlex-384 and FirePlex-1536 assays are compatible with high content imagers from various prominent manufacturers, including Molecular Devices, Perkin Elmer, GE, Yokogawa, Agilent, and Thermo. Check the required characteristics in Figure 2 below.

Figure 2. A. Varying intensities of green and yellow fluorescent dyes are used for particle identity barcoding; yellow dye, which is co-locally separate on the particle from the barcoding region, is used for analyte quantitation. B. Two images are captured per well, with approximately 20 particles analyzed per analyte using the FirePlex Analysis Workbench software. C. Representative graph demonstrates analyte fluorescent intensity levels automatically output from scanned wells along with analyte standard curves. D. List of required specifications for high-content imagers.  

​FirePlex Imager Setup Kit (ab229130) will help facilitate instrument set-up by identifying a compatible high content imager.

Don't have a high content imager? Check out our sample testing services.

Interested in FirePlex high-throughput immunoassays? Fill out an online inquiry form.

Choose the suitable high-throughput FirePlex assay format

The FirePlex-384 assays are available in three different formats: Standard, Mix and Match, and Full Custom panels. The FirePlex-1536 assays exist in two formats:  Mix and Match panels and Full custom panels.​​

Standard FirePlex-384 panels

These ready-to-use, pre-designed FirePlex-384 assay kits are comprehensively validated and contain all reagents needed to perform a 384-well assay.

Browse all FirePlex-384 immunoassay panels

​

Also available:

• For tissue lysate preparation, use a FirePlex intracellular immunoassay core kit (ab239454).
• Human Rheumatoid factor and IgM Clean-Up Solution (ab215121) helps to alleviate interference from these proteins in serum/plasma samples.

Get in touch with us if you're interested in FirePlex immunoassays.

Mix and Match panels

These panels are designed and tested with multiplexing in mind. In addition to using the unique properties of FirePlex particles to support fully flexible multiplex panels, FirePlex assays are also carefully tested for analyte multiplex interference and assay compatibility. This means that almost all our tested assays can be assembled into multiplex panels freely and without restriction.

 Select analytes of interest from existing panels with Mix and Match assay designer to build the assay that best meets your needs:

• Pick a perfect panel from a list of ever-expanding antibody pairs
• Select up to 10 analytes for FirePlex-384 and up to 5 analytes for FirePlex-1536
• Filter by species: Human, Mouse

Custom panel options

 When you need to quantify a specific set of cytokines, the choices for selecting a multiplex panel are often inflexible or inconvenient. Unique FirePlex particle technology enables us to build fully customizable panels for cytokine analysis.

Order a custom panel.

Case study: Profiling of secreted proteins from iPSCs with FirePlex-384

​Watch below D. Paul Wylie discuss how researchers profiled secreted proteins from iPSCs using the FirePlex-384 immunoassay ​or download a printable case study here.

​Recent publications featuring FirePlex-384 immunoassays

1. Gutbier et al. Large-scale production of human iPSC-derived macrophages for drug screening. Int. J. Mol. Sci. 21, 4808 (2020).
2. White et al. A multi-phenotype system to discover therapies for age-related dysregulation of the immune response to viral infections. 
3. Guide to multiplex assays in drug discovery
4. FirePlex-384 poster
5. FirePlex-384 immunoassay leaflet​
6. ​Fireplex-384 case study