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High-throughput FirePlex Immunoassays

Related

  • Multiplex assays
    • FirePlex immunoassays
      • FirePlex-96 immunoassays
        • FirePlex-384 immunoassays
          • FirePlex-1536 immunoassays
          • FirePlex Analysis Workbench software
            • FirePlex Core Technology

              High-throughput features, without compromising high-performance multiplex analysis.

              FirePlex high-throughput immunoassays, FirePlex-384 and FirePlex-1536 offer the high standards of performance and multiplexing you expect from our original FirePlex immunoassays, now in a high-throughput, automation-friendly format.

              Ideal for discovery, screening, and profiling projects, high-throughput Fireplex immunoassays offers you a fast, easy, and cost-effective tool to analyze thousands of samples rapidly. Reach your goals faster, accelerate your path to success with FirePlex immunoassays.

              ​

              NEW automation-friendly assays to accelerate your immunoassay workflow:

              • 384-well and 1536-well plate assay format
              • Simple, no-wash, two-step (384-well) ore one-step (1586-well)  workflow
              • Readout on high-content imager, < 20 min scan/384-well plate, about 1 hour/1536-well plate
              • Analyze up to 10 analytes per well in the 384-well and up to 5 analytes per well in the 1536-well assay


              Contents

              • Accelerate your research
              • Assay workflow
              • Drug development applications
              • Scale-up without sacrificing performance
              • Flexibility in analytes

              ​Accelerate your research

              FirePlex immunoassays use the FirePlex particle technology and our validated antibody pairs to deliver rapid, flexible, robust, and sensitive high-throughput multiplex screening without the need for dedicated instrumentation. Using your high content imager, you can analyze up to 10 analytes per well with FirePlex-384 and up to 6 analytes per well with FirePlex-1536, with the whole process taking less than 20 minutes per plate.

              Learn more about FirePlex-384 or FirePlex-1536 immunoassays.

              The assay workflow

              Figure 1. The assay workflow. To capture analytes onto FirePlex particles, biological samples are added to a 384-well imaging plate and incubated with the FirePlex-384 Reagent Mix overnight. Subsequently, an Imaging Dye is added, and plates are scanned on high-content imagers. The FirePlex Analysis Workbench software generates standard curves and quantifies analytes of interest directly from image files.

              ​

              ​Drug development applications

              High throughput FirePlex immunoassays can be applied at all stages of drug development to inform critical decision points. By providing a new, more effective, and affordable biomarker analysis workflow, FirePlex immunoassays enable data-driven R&D to guide the most effective strategies and minimize the risk of late-stage failure.​

              • Discovery projects screening large compound libraries
              • Lead optimization, safety assessment, and toxicity screening in large sample sets
              • Clinical screening for disease identification, patient stratification, or therapy response in large patient cohorts

              Scale-up without sacrificing performance

              With high-throughput FirePlex immunoassays, there is no trade-off between quantity and quality. Achieve high throughput without sacrificing assay performance or data quality.

              • 1-100 pg/mL sensitivity (analyte dependent)
              • 2.5-3 logs dynamic range
              • Only 6.5 µL biofluid input for 384-well plate (plasma, serum, or cell culture supernatant); 2.5 µL biofluid input for 1536-well plate (Cell culture supernatant)
              • <15% intra-plate CVs
              • Recombinant antibody pairs eliminate lot-to-lot variability
              • 70-130% sample recovery
              • Reread plates up to a week later, as many times as needed

              ​Flexibility in analytes

              Choose from: 

              • Pre-designed standard panels, Mix and Match panels, or custom panel options
              • Top 50 key cytokines and subsets of critical organ toxicity markers 
              • Human and mouse analytes


              Explore FirePlex-384 standard panels

              Explore FirePlex-384 and FirePlex-1536 Mix and Match panels in our assay designer


              To enquire about FirePlex-384 or FirePlex-1536 custom panels, contact us at fireplexsales@abcam.com.


              Learn more with our High-throughput FirePlex flyer.

              Download our FirePlex high-throughput poster

              Explore FirePlex-384 standard panels

              Explore FirePlex-384 and FirePlex-1536 Mix and Match panels in our assay designer

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