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The FirePlex® miRNA assay enables biomarker discovery and verification studies directly from small volumes of biofluid, without need for RNA purification or pre-amplification.
1. Benchmarking the FirePlex miRNA assay
2. FirePlex miRNA assays FAQ
3. RNA purification sample preparation guidelines
4. Equipment for FirePlex miRNA assays
5. FirePlex miRNA assay controls
6. How does the FirePlex miRNA work?
7. MicroRNA profiling methods: comparison of current technologies
8. MicroRNA profiling direct from biofluids, using FirePlex particle technology
9. FirePlex miRNA disease focus panels
FirePlex miRNA assay guide
A customizable biomarker development platform
The FirePlex miRNA assay is designed for both discovery and verification studies. Select between pre-designed panels for key research areas, larger discovery panels, or design custom panels to detect any miRNA from any species, whether annotated in miRBase or not.
Direct from crude biofluid samples
Robust miRNA profiles can be obtained directly from crude sample types including serum, plasma, crude exosomes, FFPEs, cell suspensions, urine and saliva. Measurement directly from biofluids using the FirePlex miRNA assay prevents sample bias, simplifies assay workflow and reduces running time.
Figure 1. Assay sensitivity in crude biofluid and purified RNA. To demonstrate the high sensitivity of the FirePlex miRNA assay, we measured the number of miRNAs detected from varying input amounts of pooled human serum or pooled RNA from human brain, lung and liver samples. We robustly detect most targets from the 48-plex panel in as little as 10 µL of serum or 100 pg of RNA.
Small sample input
Profile miRNAs even when sample is in short supply. Detect up to 65 miRNAs per well with PCR sensitivity from 10 µL biofluid or 100 pg RNA inputs.
To aid custom panel design, we provide an online tool – the FirePlex Discovery Engine. This allows you to perform species-specific literature searches to identify miRNAs implicated in diseases or research areas of interest.
In addition, our integrated FirePlex Analysis Workbench software enables easy and rapid data analysis, visualization, and export in under ten minutes, and includes key features such as standard curve analysis and publication-quality heat maps and graphs.
The full potential of miRNA profiling is currently limited by assay platform performance standards. Current challenges include the limited throughput of existing validation technologies, errors introduced during sample purification and insufficient methods used for analytical normalization and quality control.
FirePlex particle technology provides new solutions to these limitations, finally enabling researchers to rapidly and cost-effectively evaluate miRNA biomarker signatures directly from biofluids, and in solid tissues. With the capability for high-performance, high-throughput multiplexing and readout on a standard flow cytometer, the true potential of miRNA profiling is now accessible to the scientific community.
In this technical note, we demonstrate the excellent reproducibility of FirePlex miRNA assays, even with diminishing RNA input, with equivalent or better performance compared to currently available miRNA profiling technologies.
You can also learn more about miRNA research, here.
Contact us at FirePlexSales@abcam.com