High-throughput screening: ELISA-based solutions
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Accelerate your high-throughput screening (HTS) and drug discovery workflow using our ELISA-based tools and reagents for your assay development.
Reviewed February 18, 2022
Getting the most out of your HTS and drug discovery requires optimal assay development to generate the largest volume of data in the shortest possible time. Our HTS tools and reagents are specially developed to ensure that you get rapid, reproducible, and reliable results with every screening run you carry out. All our assay kits and reagents are flexible; they can be used on multiple platforms and adapted to automatable systems and liquid handlers.
Find out more about our ELISA-based HTS product range, including 384-well SimpleStep ELISA®, carrier-free antibodies, and antibody pairs.
Contents
SimpleStep ELISA®
SimpleStep ELISA® (SSE) kits are sandwich ELISA assays developed to be as simple and effective as possible, covering a range of over 900 key targets across research areas, including immunology, immuno-oncology, and neuroscience. The single-wash protocol designed for our SSE kits allows you to generate data in just 90 minutes.
Our SimpleStep ELISA kits are adaptable in a 384-well plate format to help scale up your research and provide a flexible solution that can be used on many standard plate readers. The 384-well plate SimpleStep ELISA uses the same quick and easy-to-follow one-wash protocol as our standard SimpleStep ELISA kits but requires less of your sample to run each plate.
To ensure you achieve the consistent, reliable results your research demands from your first experiment, our SSE kits contain recombinantly manufactured antibodies.
Key benefits of SimpleStep ELISA for HTS:
- Standardized 90-minute protocol across all kits
- Semi-homogenous: only one wash
- Consistency of results with low inter and intra variability
- Each kit contains high-quality recombinant antibodies for high sensitivity and reproducibility
- Adaptable in a 384-well plate format and can be used with automated liquid handling systems
View our comprehensive range of SimpleStep ELISA kits here
Learn more about SimpleStep ELISA kits here
Find more technical information about our 384-well SimpleStep ELISA Microplate here
Antibody pairs
Carrier-free antibodies and antibody pairs
We've reformulated over 10,000 of our antibodies in a carrier-free format – BSA, azide, and glycerol-free – making them ideal for customization, antibody labeling, and functional assays.
We now have over 4,000 matched antibody pairs and their individual capture and detector antibodies available in a carrier-free format, giving you the flexibility you need to adapt your pair to your HTS assay of choice. This allows you to immediately access a wide variety of antibody pairs in an entirely customizable format. The range of carrier-free pairs on our catalog grows every year to give you access to the target you need.
You are not limited to ELISA assays; matched antibody pair kits and carrier-free pairs can be used in other assays adapted to high-throughput scale, such as fluorescent proximity assays (Time-Resolved Fluorescence Energy Transfer [TR-FRET]). Each pair is based on recombinant monoclonal antibodies for the highest level of batch-to-batch consistency between each of your screening runs.
View all carrier-free, recombinant antibodies
View all carrier-free matched antibody pairs
Find out more about carrier-free antibodies and antibody pairs here
Key benefits of our antibody pairs for HTS:
- Extensive target coverage
- Recombinantly manufactured for the highest level of consistency between batches and security of supply
- Can be used in other types of HTS immunoassays (eg TR-FRET), offering a flexible solution
Multiplex immunoassays
If ELISA-based HTS solutions are not what you need, we also offer high-performance multiplex immunoassays, including high-throughput automatable formats FirePlex®-384 and FirePlex®-1536 suitable for drug discovery and high-throughput compound and biomarker screening.
Find out more about our high-throughput multiplex immunoassays, FirePlex-384, and FirePlex-1536.
See in detail how SimpleStep ELISA® in 384-well format can be used in conjunction with the PHERAstar FSX to quantify Human IgG and human IFN gamma, two important targets in pharmacokinetic studies and immunotherapeutic drug safety testing.
Explore how our MAP kits can be used in a 384-well Time-Resolved-Fluorescence (TRF) immuno-assay and read on the PHERAstar FSX for the detection IRAK4, a protein kinase involved in cell signaling as part of the immune response.
Contact the sales team
If you're interested in obtaining a quote for any of the products mentioned above or would like to discuss our solutions with the sales team, submit your details using our short form. A member of our team would be happy to discuss how we can support your research.
FirePlex® is a registered trademark in certain territories (including Europe and the United States) and is an unregistered trademark elsewhere.