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High-throughput screening: ELISA-based solutions

Related

  • ELISA and matched antibody pair kits
    • ELISA guide
      • Browse SimpleStep ELISA kits
        • SimpleStep ELISA
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                      Accelerate your high-throughput screening (HTS) and drug discovery workflow using our ELISA-based tools and reagents for your assay development.

                      Reviewed February 18, 2022

                      Getting the most out of your HTS and drug discovery requires optimal assay development to generate the largest volume of data in the shortest possible time. Our HTS tools and reagents are specially developed to ensure that you get rapid, reproducible, and reliable results with every screening run you carry out. All our assay kits and reagents are flexible; they can be used on multiple platforms and adapted to automatable systems and liquid handlers. 

                      Find out more about our ELISA-based HTS product range, including 384-well SimpleStep ELISA®,  carrier-free antibodies, and antibody pairs.

                      Contents

                      • SimpleStep ELISA®
                        • ​Key benefits of SimpleStep ELISA for HTS
                      • Antibody pairs
                        • ​Carrier-free antibodies and antibody pairs
                        • ​​Key benefits of our antibody pairs for HTS
                      • ​Read our application notes
                      • Contact the sales team 


                      SimpleStep ELISA® 

                      SimpleStep ELISA® (SSE) kits are sandwich ELISA assays developed to be as simple and effective as possible, covering a range of over 900 key targets across research areas, including immunology, immuno-oncology, and neuroscience. The single-wash protocol designed for our SSE kits allows you to generate data in just 90 minutes.  

                      Our SimpleStep ELISA kits are adaptable in a 384-well plate format to help scale up your research and provide a flexible solution that can be used on many standard plate readers. The 384-well plate SimpleStep ELISA uses the same quick and easy-to-follow one-wash protocol as our standard SimpleStep ELISA kits but requires less of your sample to run each plate. 

                      To ensure you achieve the consistent, reliable results your research demands from your first experiment, our SSE kits contain recombinantly manufactured antibodies.

                      Key benefits of SimpleStep ELISA for HTS: 

                      • Standardized 90-minute protocol across all kits 
                      • Semi-homogenous: only one wash 
                      • Consistency of results with low inter and intra variability 
                      • Each kit contains high-quality recombinant antibodies for high sensitivity and reproducibility  
                      • Adaptable in a 384-well plate format and can be used with automated liquid handling systems 

                      View our comprehensive range of SimpleStep ELISA kits here 

                      Learn more about SimpleStep ELISA kits here 

                      Find more technical information about our 384-well SimpleStep ELISA Microplate here 

                      Antibody pairs 

                      Carrier-free antibodies and antibody pairs 

                      We've reformulated over 10,000 of our antibodies in a carrier-free format – BSA, azide, and glycerol-free – making them ideal for customization, antibody labeling, and functional assays.  

                      We now have over 4,000 matched antibody pairs and their individual capture and detector antibodies available in a carrier-free format, giving you the flexibility you need to adapt your pair to your HTS assay of choice. This allows you to immediately access a wide variety of antibody pairs in an entirely customizable format. The range of carrier-free pairs on our catalog grows every year to give you access to the target you need. 

                      You are not limited to ELISA assays; matched antibody pair kits and carrier-free pairs can be used in other assays adapted to high-throughput scale, such as fluorescent proximity assays (Time-Resolved Fluorescence Energy Transfer [TR-FRET]).   Each pair is based on recombinant monoclonal antibodies for the highest level of batch-to-batch consistency between each of your screening runs. 

                      View all carrier-free, recombinant antibodies 

                      View all carrier-free matched antibody pairs  

                      Find out more about carrier-free antibodies and antibody pairs here 

                      Key benefits of our antibody pairs for HTS: 

                      • Extensive target coverage 
                      • Recombinantly manufactured for the highest level of consistency between batches and security of supply  
                      • Can be used in other types of HTS immunoassays (eg TR-FRET), offering a flexible solution ​

                      Read our application notes

                      ​See in detail how SimpleStep ELISA® in 384-well format can be used in conjunction with the PHERAstar FSX to quantify Human IgG and human IFN gamma, two important targets in pharmacokinetic studies and immunotherapeutic drug safety testing.

                      Application note for high-throughput 90-minute ELISA: 384-well SimpleStep ELISA® with the PHERAstar FSX

                      Explore how our MAP kits can be used in a 384-well Time-Resolved-Fluorescence (TRF) immuno-assay and read on the PHERAstar FSX for the detection IRAK4, a protein kinase involved in cell signaling as part of the immune response.

                      Application note for 384-well microplate Time-Resolved Fluorescence (TRF) immunoassay using a matched antibody pair kit and the PHERAstar FSX

                      how SimpleStep ELISA® in 384-well format can be used in conjunction
                      with the PHERAstar FSX to quantify Human IgG and human IFN gamma, two important
                      targets in pharmacokinetic studies and immunotherapeutic drug safety testing.

                      Contact the sales team  

                      If you're interested in obtaining a quote for any of the products mentioned above or would like to discuss our solutions with the sales team, submit your details using our short form. A member of our team would be happy to discuss how we can support your research.  

                      Submit an inquiry

                       




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