Human analytes and panels for multiplex immunoassays

Related

​​View human analytes and panels for the multiplex immunoassays.

Our Firefly​™ multiplex immunoassays are currently in beta testing. For more information, or to find out how to take part and receive a free kit, contact sales@abcam.com.

This page has been replaced. Please view an up-to-date list of human analytes and panels for FirePlex immunoassays.

NB Firefly multiplex immunoassays are now known as FirePlex immunoassays.


Our multiplex immunoassays can be used to quantify many cytokines or other proteins/peptides at the same time. Either send us your samples to get effortless results or run the assays in your own lab and analyze using your flow cytometer.

  • Check the analyte and panel list below
  • Download a pdf listing
  • View pipeline of analytes coming soon

Whether you are using our services or our kits, you can opt for either a pre-designed panel or assemble your own panel. Pre-designed panels can also be extended with extra analytes. We've carefully designed the assays so you can quantify all your analytes in the same well, however exceptions (and recommended sample dilutions) are highlighted at the bottom of this page.

All human analytes are validated for use with serum, plasma (EDTA, heparin, citrate), cell culture supernatant, urine, saliva, milk (defatted), bronchial lavage (BAL), cerebrospinal fluid (CSF) and synovial fluid samples.

Pre-designed panels


PanelPlexAnalytes
Key cytokines15GM-CSF, IFN-gamma, IL-1 beta, IL-2, IL-4, IL-5, IL-6, IL-8, IL-9, IL-10, IL-12 p70, IL-13, IL-17A, MCP1, TNF alpha
Th1/Th2/Th177
IL-2, IL-4, IL-6, IL-10, TNF alpha, IFN gamma, IL-17A
Th1/Th27IL2, IL4, IL5, IL6, IL10, TNF alpha, IFN gamma
Inflammation
8
IFN-gamma, IL-1 beta, IL-6, IL-8, IL-10, IL-12p70, TNF alpha, MCP1
Key cytokines4IFN-gamma, IL-6, IL-10, TNF-alpha


List of human analytes



AbID

Dynamic Range* (pg/mL)

Limit of Detection* (pg/mL)

low

high

alpha Fetoproteinab21338741.290,0006.32

BCA1

ab208220

4.6

10,000

0.79

CEAab213386123.5270,00020.2
CD40 Ligandab2133821.53,3330.44
CRPab2133774.610,0000.87
CXCL5ab2133744.610,0000.65

CXCL16

ab213374

4.6

10,000

0.65

Eotaxin

ab208213

1.5

3,333

0.49

Fibrinogenab213385123.5270,00052.66

Fractalkine

ab208223

1.5

3,333

0.29

G-CSF

ab208218

4.6

10,000

1.78

GM-CSF

ab208216

4.6

10,000

2.13

GRO alpha

ab209509

1.5

3,333

0.29

ICAM1ab21338113.730,0001.85

IFN-gamma

ab209504

4.6

10,000

0.67

IL-1 beta

ab209502

1.5

3,333

0.46

IL-1a

ab209844

1.5

3,333

1.19

IL-1Ra

ab208224

1.5

3,333

0.76

IL-2

ab208207

4.6

10,000

4.05

IL-4

ab208208

4.6

10,000


1.23

IL-5

ab208225

1.5

3,333

0.5

IL-6

ab208206

4.6

10,000

2.46

IL-6 R alpha

ab208217

13.7

30,000

4.62

IL-7

ab208226

1.5

3,333

0.74

IL-8

ab208214

1.5

3,333

0.34

IL-9

ab209506

1.5

3,333

0.51

IL-10ab2082094.610,0001.76
IL-12p70ab2095051.53,3331.06
IL-13ab2082104.610,0002.52
IL-16ab21337513.730,0003.72
IL-17Aab2082154.610,0002.88
IL-17Fab2133804.610,0000.98
IL-23ab2095084.610,0001.94

IP-10

ab209503

1.5

3,333

0.2

Leptinab2133794.610,00010.7

MCP1

ab208211

1.5

3,333

0.44

MCP3ab2133724.610,0000.8

MDC

ab208222

4.6

10,000

0.79

MIP1 alpha

ab208228

1.5

3,333

0.23

MIP1 beta

ab208227

1.5

3,333

0.89

MIP2ab2082211.53,3330.44
PF4ab2133734.610,0001.45
tPSAab2133831.53,3330.25

RANTES


ab208212

1.5

3,333

0.77

Serum Albuminab21337641.590,00014.38

TARC

ab208219

13.7

30,000

0.79

TNF-alpha

ab208205

4.6

10,000

1.37




 FirePlex® is a registered trade mark in the United States and is an unregistered trademark elsewhere.


Sign up