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Matched antibody pair kits for ELISA

Related

  • ELISA, matched antibody pairs, and multiplex immunoassays
    • Find out more about SimpleStep ELISA®
      • Browse all SimpleStep ELISA® kits
        • Browse our complete ELISA list
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                                • High-throughput screening: ELISA-based solutions

                                  ​​Create your own ELISA with high-performing antibody pairs.

                                  ​​

                                  Our antibody pairs come in 2 formats:

                                  • Antibody pair kits: include a titrated capture and biotinylated detector antibody pair and a calibrated protein standard. Available in two sizes, with enough reagents for either 5 or 10 x 96-well plates using a standard sandwich ELISA. 
                                  • Carrier-free antibody pairs (BSA, glycerol, and Azide free): include a capture and detector antibody pair, suitable for ELISA-based assays. Available as 10 x 96-wells plate. Individual capture and detector antibodies can also be purchased separately in 100µg and 1mg sizes.

                                  Learn more about the advantages of carrier-free antibodies and antibody pairs here

                                  Antibody pair kits and reagents deliver consistent, specific, and sensitive results.

                                  Batch-to-batch consistency: only recombinant monoclonal antibodies are used in our antibody pairs.

                                  Specificity: antibody pairs are screened in plasma and serum to ensure specificity in complex samples.

                                  Sensitivity: benchmarked against commercially available antibody pairs to ensure equivalent or superior performance compared with the competition.

                                  Flexibility: Our carrier-free pairs offer even more flexibility in terms of conjugation and assay set-up.

                                  High-throughput screening (HTS) and antibody pairs
                                  Getting the most out of your HTS and drug discovery requires optimal assay development to generate the largest volume of data in the shortest possible time. Our antibody pairs are ideal for tailoring your ELISA assays to your own plate systems; they can be used in 96 or 384-well formats to scale up your HTS. Our antibody pairs are extremely flexible; they can be used with many different platforms and run using standard plate readers.

                                  You are also not limited to ELISA assays; MAP kits can be used in other assays adapted to high-throughput scale such as fluorescent proximity assays (TR-FRET: Time-Resolved Fluorescence Energy Transfer).  With 300 MAP kits and over 900 carrier-free antibody pairs, you get immediate access to a huge variety of pairs in a completely customizable format. The range of carrier-free pairs on our catalog is also growing every year to give you access to the target you need. For extra flexibility, the capture and detector antibodies can also be purchased separately. 


                                  Find out more about our ELISA-based high-throughput screening solutions here 

                                  See the range

                                  Figure 1. The  human IL-1Ra matched antibody pair kit (ab210888) is 10-fold more sensitive than a rabbit polyclonal antibody pair when measuring human IL-1Ra standard protein using a sandwich ELISA.

                                  See more comparison data

                                  ELISA guide

                                  >> Next page: Choose the right ELISA kit.

                                  << Previous page: SimpleStep ELISA® kits

                                  Download the full guide


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