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Mitochondrial assays

Related

  • Metabolism assays
    • ROS, oxidative stress, and antioxidants
      • Metabolism research resources
        • Mitosciences antibodies
          • Oxidative phosphorylation pathway

            Simple assays and biochemicals for your mitochondria and metabolism research.

            Review our assays for mitochondrial research:

            • Mitochondrial biogenesis
            • Mitochondrial viability
            • Mitochondrial toxicity
            • OXPHOS enzyme complex activity assays
            • OXPHOS protein expression
            • Apoptosis

            For more detailed information about the different methods used to analyze mitochondria, see our mitochondrial toxicity application guide.

            ​

            Mitochondrial biogenesis

            Measurement of mitochondrial biogenesis is becoming a standard part of drug safety characterization. Our assays measure biogenesis by measuring the ratio of mitochondrial and nuclear-encoded proteins.

            Assay

            Assay kits

            In-Cell ELISA with fluorescence, colorimetric or fluorometric readout

            Western blot antibody cocktail

            ICC antibody cocktail

            Flow cytometry antibody cocktail

            ab110217, ab110216, ab140359


            ​ab123545

            ab170194

            ab168540

             “I am using this kit [ab110217] for high throughput screening of more than 500 compounds. The kit is highly reproducible and I did not observe any lot to lot variation. I would highly recommend this kit.”

            – Dr Analeeb Sajid

            Cox1 SDHA v1

            ​

            Mitochondrial viability

            The mitochondrial membrane potential is a key indicator of mitochondria activity levels. A number of fluorescent dyes are available that accumulate in mitochondria due to the membrane potential.

            Assay

            Readout

            Notes

            Assay kits

            TMRE/ TMRM

            Plate reader, microscope, flow cytometer

            Most popular Abcam mitochondrial membrane dye assay.
            Ex/Em 549/575 nm. Washed out after fixation.

            ab113852

            JC-1/ JC-10

            JC-1 (Ex/Em 530/530–570) and JC-10 (Ex/Em 590/520–570) form red aggregates at high concentrations (unaggregated dye is green). Loss of membrane potential causes loss of dye and increased green fluorescence.

            Washed out after fixation.

            JC-1:
            ab113850

            JC-10
            (more soluble than JC-1):
            ab112134
            ab112133

            MitoNIR

            Plate reader, flow cytometer

            Ex/Em 635/660.

            ab112149 ab11250

            Mito Orange

            Ex/Em 540/590.

            ab138898 ab138899

            JC1 web v1

            ​​

            Mitochondrial toxicity

            ​Your complete toolkit for investigating mitochondrial toxicity in vitro.

            Every year, dozens of pharmaceutical compounds are withdrawn from the market due to adverse effects not discovered during the preclinical or clinical testing. Many of these adverse effects are subtle and do not lead to visible histopathology, but often lead to mitochondrial dysfunction. Therefore, screening for mitochondrial toxicity in the early development stages of a drug is essential.
            • Don't know where to start? Our step-by-step guide will help you to find the right assay for you.
            • Download a copy of our Mitochondrial toxicity Application Guide
            • Looking for the right assay? This guide will help you choose the key parameters of mitochondrial toxicity.
            • Download a copy of our Tools for Detecting Mitochondrial Toxicity

            OXPHOS enzyme complex activity assays

            To investigate the in vitro activity of individual oxidative phosphorylation (OXPHOS) enzyme complexes, we use immunocapture to enable specific biochemical assays for each complex.


            Immunocapture activity assay

            Complex immunocapture

            Toxicity assay

            Notes

            Capture complex and assay activity

            Enable user to develop own assays based on captured complex

            Immunocapture purified beef mitochondria for in vitro treatment and assays

            Complex I

            ab109721

            ab109711

            ab109903

            Complex II

            ab109908

            ab109799

            ab109904

            Complex III


            ab109800

            ab109905 (II + III)

            Complex IV

            ab109911, ab109909, ab109910

            ab109801, ab109860

            ab109906

            Complex V



            ab109907

            Complete panel



            ab110419

            rhoa web v2

            OXPHOS protein expression analysis

            We have developed antibody cocktails for use in western blotting to enable analysis of all 5 oxphos protein complexes in a single experiment.

            Assay

            Assay kits

            Complex I-V antibody cocktail

            ab110413, ab110411, ab110412

            Complex II antibody cocktail

            ab110410

            ​

            ​Apoptosis

            Mitochondria are closely involved in apoptosis and cell death. For an in depth look at apoptosis see our Apoptosis Application Guide or to review available assays, tools and reagents, see our Cell Health Assays Guide.



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