Mitochondrial assays
Related
Simple assays and biochemicals for your mitochondria and metabolism research.
Review our assays for mitochondrial research:
- Mitochondrial biogenesis
- Mitochondrial viability
- Mitochondrial toxicity
- OXPHOS enzyme complex activity assays
- OXPHOS protein expression
- Apoptosis
For more detailed information about the different methods used to analyze mitochondria, see our mitochondrial toxicity application guide.
Mitochondrial biogenesis
Measurement of mitochondrial biogenesis is becoming a standard part of drug safety characterization. Our assays measure biogenesis by measuring the ratio of mitochondrial and nuclear-encoded proteins.
Assay | Assay kits |
In-Cell ELISA with fluorescence, colorimetric or fluorometric readout Western blot antibody cocktail ICC antibody cocktail Flow cytometry antibody cocktail |
“I am using this kit [ab110217] for high throughput screening of more than 500 compounds. The kit is highly reproducible and I did not observe any lot to lot variation. I would highly recommend this kit.”
– Dr Analeeb Sajid
Mitochondrial viability
The mitochondrial membrane potential is a key indicator of mitochondria activity levels. A number of fluorescent dyes are available that accumulate in mitochondria due to the membrane potential.
Assay | Readout | Notes | Assay kits |
TMRE/ TMRM | Plate reader, microscope, flow cytometer | Most popular Abcam mitochondrial membrane dye assay. | |
JC-1/ JC-10 | JC-1 (Ex/Em 530/530–570) and JC-10 (Ex/Em 590/520–570) form red aggregates at high concentrations (unaggregated dye is green). Loss of membrane potential causes loss of dye and increased green fluorescence. Washed out after fixation. | JC-1: | |
MitoNIR | Plate reader, flow cytometer | Ex/Em 635/660. | |
Mito Orange | Ex/Em 540/590. |
Mitochondrial toxicity
Your complete toolkit for investigating mitochondrial toxicity in vitro.
• Don't know where to start? Our step-by-step guide will help you to find the right assay for you.
• Download a copy of our Mitochondrial toxicity Application Guide
• Download a copy of our Tools for Detecting Mitochondrial Toxicity
OXPHOS enzyme complex activity assays
To investigate the in vitro activity of individual oxidative phosphorylation (OXPHOS) enzyme complexes, we use immunocapture to enable specific biochemical assays for each complex.
Immunocapture activity assay | Complex immunocapture | Toxicity assay | |
Notes | Capture complex and assay activity | Enable user to develop own assays based on captured complex | Immunocapture purified beef mitochondria for in vitro treatment and assays |
Complex I | |||
Complex II | |||
Complex III | ab109905 (II + III) | ||
Complex IV | |||
Complex V | |||
Complete panel |
OXPHOS protein expression analysis
We have developed antibody cocktails for use in western blotting to enable analysis of all 5 oxphos protein complexes in a single experiment.
Assay | Assay kits |
Complex I-V antibody cocktail | |
Complex II antibody cocktail |
Apoptosis
Mitochondria are closely involved in apoptosis and cell death. For an in depth look at apoptosis see our Apoptosis Application Guide or to review available assays, tools and reagents, see our Cell Health Assays Guide.