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Abcam has been developing assays for mitochondrial research since 2004.
Review our assays for mitochondrial research:
For more detailed information about the different methods used to analyze mitochondria, see our mitochondrial toxicity application guide.
Measurement of mitochondrial biogenesis is becoming a standard part of drug safety characterization. Our assays measure biogenesis by measuring the ratio of mitochondrial and nuclear-encoded proteins.
In-Cell ELISA with fluorescence, colorimetric or fluorometric readout
Western blot antibody cocktail
ICC antibody cocktail
Flow cytometry antibody cocktail
“I am using this kit [ab110217] for high throughput screening of more than 500 compounds. The kit is highly reproducible and I did not observe any lot to lot variation. I would highly recommend this kit.”
– Dr Analeeb Sajid
The mitochondrial membrane potential is a key indicator of mitochondria activity levels. A number of fluorescent dyes are available that accumulate in mitochondria due to the membrane potential.
Plate reader, microscope, flow cytometer
Most popular Abcam mitochondrial membrane dye assay.
JC-1 (Ex/Em 530/530–570) and JC-10 (Ex/Em 590/520–570) form red aggregates at high concentrations (unaggregated dye is green). Loss of membrane potential causes loss of dye and increased green fluorescence.
Washed out after fixation.
Plate reader, flow cytometer
To investigate the in vitro activity of individual oxidative phosphorylation (OXPHOS) enzyme complexes, we use immunocapture to enable specific biochemical assays for each complex.
Immunocapture activity assay
Capture complex and assay activity
Enable user to develop own assays based on captured complex
Immunocapture purified beef mitochondria for in vitro treatment and assays
ab109905 (II + III)
We have developed antibody cocktails for use in western blotting to enable analysis of all 5 oxphos protein complexes in a single experiment.
Complex I-V antibody cocktail
Complex II antibody cocktail
Mitochondria are closely involved in apoptosis and cell death. For an in depth look at apoptosis see our Apoptosis Application Guide or to review available assays, tools and reagents, see our Cell Health Assays Guide.