Multicolor enzymatic IHC

Designed for optimal staining of two or three antigens on human or animal tissue sections, our multicolor IHC detection kits offer you the following:

• Reduced background
  • when staining tissue containing endogenous biotin (eg kidney, liver and spleen) using an HRP and AP polymer based system
• Optimized protocol
  • for simplified set up and development of multicolor experiments
• Better signal to noise ratio
  • for clear visualization of staining and morphology
• Flexible color combinations
  • based on red,  green, purple, brown and black
• Use of rodent antibodies on rodent tissue
  • protocols for blocking endogenous mouse IgG or rat IgG
• May be combined with histology special stains where compatible

Why enzymatic staining?

Although multicolor IHC using immunofluorescence is a well-established technique for visualizing multiple antigens it presents several challenges including:

• Quenching of fluorescent signal at excitation
• Fading of the fluorescence signal during slide storage
• Occurrence of autofluorescence due to formaldehyde fixation
• Restricted combinations with special stains to identify tissue structures or microorganisms

For staining two markers: View DoubleStain multicolor IHC kits

For staining three markers: View TripleStain multicolor IHC kits