Multicolor enzymatic IHC
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Advanced multicolor enzymatic IHC kits.
Designed for optimal staining of two or three antigens on human or animal tissue sections, our multicolor IHC detection kits offer you the following:
- Reduced background
- when staining tissue containing endogenous biotin (eg kidney, liver and spleen) using an HRP and AP polymer based system
- Optimized protocol
- for simplified set up and development of multicolor experiments
- Better signal to noise ratio
- for clear visualization of staining and morphology
- Flexible color combinations
- based on red, green, purple, brown and black
- Use of rodent antibodies on rodent tissue
- protocols for blocking endogenous mouse IgG or rat IgG
- May be combined with histology special stains where compatible
Why enzymatic staining?
Although multicolor IHC using immunofluorescence is a well-established technique for visualizing multiple antigens it presents several challenges including:
- Quenching of fluorescent signal at excitation
- Fading of the fluorescence signal during slide storage
- Occurrence of autofluorescence due to formaldehyde fixation
- Restricted combinations with special stains to identify tissue structures or microorganisms
For staining two markers: View DoubleStain multicolor IHC kits
For staining three markers: View TripleStain multicolor IHC kits