All tags FirePlex How FirePlex® immunoassays work

Firefly Multiplex Immunoassay Hero

How FirePlex® immunoassays work

Accelerate your research while minimizing your workload with high-performance, high-throughput multiplexing capabilities.

Take your biomarker research to the next level with FirePlex immunoassays. FirePlex particle technology and assay workflow give you the power to analyze far more analytes in a fraction of the time, with minimal sample input requirements.

High-throughput biomarker screening
96-well plate format 
75 analytes per well
yielding over 2,700 data points per plate
only 12.5 uL of raw sample required

Fast and easy workflow
Analyze biomarkers directly from crude biofluids, no preprocessing required
Multiplex analysis eliminates the need to divide samples across wells
Simple 5-step workflow
Streamlined wash steps using vacuum manifold
2.5 hour assay run time

Data collection and analysis simplified
Fast and easy readout on standard flow cytometers
Free-of-charge integrated FirePlex Analysis Workbench software makes complex data analysis simple. Software quickly and easily decodes the data for you, plots standard curves and delivers publication-ready figures and results.   

High-throughput biomarker screening

  • 96-well plate format 
  • 75 analytes per well
  • yielding over 2,700 data points per plate
  • only 12.5 µL of raw sample required

Fast and easy workflow

  • Analyze biomarkers directly from crude biofluids, no preprocessing required
  • Multiplex analysis eliminates the need to divide samples across wells
  • Simple 5-step workflow
  • Streamlined wash steps using a vacuum manifold
  • 2.5-hour assay run time

Data collection and analysis simplified

  • Fast and easy readout on standard flow cytometers
  • Free-of-charge integrated FirePlex Analysis Workbench software makes complex data analysis simple. Software quickly and easily decodes the data for you, plots standard curves and delivers publication-ready figures and results.   

Assay worflow

The FirePlex immunoassay is run in a 96-well filter plate, streamlining wash steps using a vacuum manifold (ab204067). 

Alternatively, leave the work to us and let our full service team of experts analyze your samples from start to finish.


Compatible flow cytometers


FirePlex immunoassays can be conveniently run on your own existing flow cytometer, eliminating the need to purchase dedicated equipment. FirePlex particles are validated for use with the following flow cytometers fitted with green, yellow and red detectors.

  • Millipore's Guava EasyCyte™
  • BD Biosciences' Accuri™ C6
  • ThermoFisher's Attune® (limited analyte list; not validated for the NxT)
  • BD Biosciences' FACSCanto™, LSRII, and LSRFortessa™
    • Required laser and filter configuration for FACSCanto™, LSRII and LSRFortessa™
      • Either blue 488 nm laser excitation for green Em525, yellow Em570 and red Em667 filters, or
      • Blue laser excitation for green and red filters, and yellow/green 561 nm/532 nm laser excitation for the yellow filter
  • Our Cytometer Setup kit (ab211043) can be used to ensure cytometer is compatible and has appropriate settings.

Don't have access to a compatible flow cytometer? You can still profile cytokines with the help of our sample profiling team.


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FirePlex® is a registered trademark in the United States and is an unregistered trademark elsewhere.











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