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Quantitative antibody arrays:
Almost all gene microarray scanners that are able to scan a standard histology slide, have a Cy3 (green) channel and have pixel resolution ≤10 µm are compatible.
Contact us if you are unsure if your scanner is compatible. Illumina and Affymetrix scanners are incompatible as they are a closed system. Agilent scanners are not recommended as the detector is on the other side of the slide from the laser, rather than on the same side.
Tip: if you don’t have your own scanner look for local genomics labs or try asking a local genomics core facility.
|Slide format||Standard 1" x 3" (25 mm x 75 mm) glass microscope slides|
|Light and Detector Orientation||Facing array|
|Scanned Area||22 mm x 73 mm|
|Focus||Auto focus or adjustable (+/- 200 µm)|
|Excitation/ Emission||Cy3 (Green) Channel 532 nm|
|Dynamic Range||>3 orders of magnitude|
|Detection Output||16-bit TIFF|
AlphaScan Microarray Scanner (Alpha Innotech)
|LS Series Laser Scanner (Tecan Group AG)|
|aQuire 110V (Genetix)||NovaRay Detection Platform (Alpha Innotech)|
|aQuire 240V (Genetix)|
Open Frame DNAscope (Biomedical Photometrics)
|arrayWoRx (Applied Precision)||Revolution 4200 Microarray Scanner (VIDAR)|
|DNAscope LM (Biomedical Photometrics)||ScanArray® Lite (PerkinElmer, Inc.)|
|DNAscope IV & V (Biomedical Photometrics)||ScanArray® Express (PerkinElmer)|
GenePix® 4000B (Molecular Devices)
|ScanArray® Express HT (PerkinElmer)|
GenePix® 4100A (Molecular Devices)
|ScanArray® 4000 (PerkinElmer)|
|GenePix® Professional 4200A (Molecular Devices)||ScanArray® 4000XL (PerkinElmer)|
|GenePix® 4000A (Molecular Devices)||ScanArray® 5000 (PerkinElmer)|
|GSD-501 System Calibration Kit (Invitrogen)||ScanArray® 5000XL (PerkinElmer)|
|InnoScan 900 Microarray Scanner (Innopsys)||VersArray ChipReader 5um System (Bio-Rad)|
|InnoScan 710 Microarray Scanner (Innopsys)||VersArray ChipReader 3um System (Bio-Rad)|