SimpleStep ELISA® kits

​​​​​​​Highly sensitive 90-minutes ELISAs, with just one wash step and no compromise on performance.

• Single-wash, easy protocol reduces assay time to 90 minutes or less
• High sensitivity, specificity, and reproducibility from superior antibodies
• Fully validated in biological samples
• ​96-wells plate breakable into 12 x 8 wells strips

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​Contents

• What is SimpleStep ELISA?
• How does SimpleStep ELISA work?
• SimpleStep ELISA and the SPECTROstar^®Nano
• High-throughput screening and SimpleStep ELISA
• Uncompromising on sensitivity
• Tested in biological samples
• Analyte range

What is a SimpleStep ELISA?

SimpleStep ELISA^® kits are sandwich ELISA assays that we have developed to be as simple and effective as possible. Unlike traditional sandwich ELISAs, which take more than three hours to run, SimpleStep ELISA kits generate data in just 90 minutes, with only one wash step and without compromising performance.

Your ELISA upgrade

How does SimpleStep ELISA work?

​Figure 1. With SimpleStep ELISA kits, an analyte-capture and detector antibody sandwich complex is formed in solution, which binds to the microplate via an affinity tag attached to the capture antibody in the sandwich pair. See the video of how SimpleStep ELISA works.

A conventional sandwich ELISA uses a pair of antibodies that bind to at least two distinct sites on the target protein. The capture antibody is pre-coated onto the well of a microplate and selectively binds to the target protein. After a wash step, the detector antibody is added and binds to a second site on the target protein to form a sandwich complex.

SimpleStep ELISA kits streamline this process by using a semi-homogeneous format wherein the antibody-analyte sandwich complex is formed in solution in a single step. In just one incubation and wash step, the complete sandwich complex forms in the well and is anchored to the plate with an immunoaffinity tag (figure 1).

SimpleStep ELISA kits and the SPECTROstar^®Nano

To get the best results from your SimpleStep ELISA kits we recommend that you run your plates using the SPECTROstar^®Nano​. Reading microplates with the SPECTROstar^®Nano​ provides notable data quality while offering the convenience of linking data analysis to MARS data analysis templates, for immediate and accurate results.

Find out in this application note how you can use the SPECTROstar^®Nano​ to run your SimpleStep ELISA kit. 

Find out in this application note how you can carry out immuno-oncology biomarker measurement with high sensitivity and speed using SimpleStep ELISA^® kits and SPECTROstar^®Nano

Find out in this application note how you can achieve fast and accurate detection of Alzheimer’s disease targets with SimpleStep ELISA^® kits and SPECTROstar^®Nano

High-throughput screening (HTS) and SimpleStep ELISA

Our HTS tools and reagents are specially developed to ensure that you get rapid, reproducible, and reliable results with every screening run you carry out. Our SimpleStep ELISA kits are adaptable in a 384-well plate format and can be used with automated liquid handling systems. These plates help scale up your research and provide a flexible solution that can be used on many standard plate readers. The 384-well plate SimpleStep ELISA uses the same quick and simple one wash protocol as our standard SimpleStep ELISA kits but requires less of your sample to run each plate.

Find more technical information about our 384-well SimpleStep ELISA Microplate here

Find out more about our ELISA-based high-throughput screening solutions here

Uncompromising on sensitivity

SimpleStep ELISA kits drastically reduce assay times without compromising on performance. We compared the sensitivity of SimpleStep ELISA with that of the next best competitor brand of ELISA kits for 75 popular human and mouse proteins. In 56 out of 75 cases, SimpleStep ELISA shows superior sensitivity (figure 2).

Figure 2. We compared SimpleStep ELISA kit sensitivity with a competitor assay across 75 popular protein targets and calculated the ratios. SimpleStep ELISA was more sensitive on average than a competitor ELISA kit.

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Tested in biological samples

Every SimpleStep ELISA® kit is validated using multiple biological samples for assay specificity. All secreted serum or plasma-based targets are tested and fall within World Health Organization blood reference ranges. When available, the SimpleStep ELISA kits are calibrated against a known NIBSC international standards and includes a conversion factor for data comparison.

Analyte range

We currently have SimpleStep ELISA® kits to almost 700 targets, including popular proteins like PD-L1, GFP, and fibrinogen, and are continually adding more to the catalog.

SimpleStep ELISA® kits for the top 10 most popular ELISA targets:

* also available for rabbit, hamster, dog and guinea pigs samples

** also reacts with Rhesus Monkey

*** multispecies

Find your SimpleStep ELISA® kit​​

ELISA guide

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