SimpleStep ELISA^® kits are sandwich ELISA assays that we have developed to be as simple and effective as possible. Unlike traditional sandwich ELISAs, which take more than three hours to run, SimpleStep ELISA kits generate data in just 90 minutes, without compromising performance.

Your ELISA upgrade

SimpleStep ELISA® offers	What it means for you
High sensitivity, reproducibility and specificity.	Consistent and reliable results from the first attempt.
Validation on biological samples.	Guaranteed reaction with the endogenous protein.
Single-wash 90 minutes standardised protocol.	Less risk of error with the same fast and easy protocol across all SimpleStep ELISA® kits.
96-wells plate breakable into 12 x 8 wells strips.	No waste. Possibility to use the same kit for several smaller experiments.

How does SimpleStep ELISA work?

Figure 1. With SimpleStep ELISA kits, an analyte-capture and detector antibody sandwich complex is formed in solution, which binds to the microplate via an affinity tag attached to the capture antibody in the sandwich pair. See the video of how SimpleStep ELISA works.

A conventional sandwich ELISA uses a pair of antibodies that bind to at least two distinct sites on the target protein. The capture antibody is pre-coated onto the well of a microplate and selectively binds to the target protein. After a wash step, the detector antibody is added and binds to a second site on the target protein to form a sandwich complex.

SimpleStep ELISA kits streamline this process by using a semi-homogeneous format wherein the antibody-analyte sandwich complex is formed in solution in a single step. In just one incubation and wash step, the complete sandwich complex forms in the well and is anchored to the plate with an immunoaffinity tag (figure 1).

SimpleStep ELISA kits and the SPECTROstar^®^Nano

To get the best results from your SimpleStep ELISA kits we recommend that you run your plates using the SPECTROstar^®Nano. Reading microplates with the SPECTROstar^®Nano provides notable data quality while offering the convenience of linking data analysis to MARS data analysis templates, for immediate and accurate results.

Find out in this application note how you can use the SPECTROstar^®Nano to run your SimpleStep ELISA kit.

Find out in this application note how you can carry out immuno-oncology biomarker measurement with high sensitivity and speed using SimpleStep ELISA^® kits and SPECTROstar^®Nano

Find out in this application note how you can achieve fast and accurate detection of Alzheimer’s disease targets with SimpleStep ELISA^® kits and SPECTROstar^®Nano

High-throughput screening (HTS) and SimpleStep ELISA

Our HTS tools and reagents are specially developed to ensure that you get rapid, reproducible, and reliable results with every screening run you carry out. Our SimpleStep ELISA kits are adaptable in a 384-well plate format and can be used with automated liquid handling systems. These plates help scale up your research and provide a flexible solution that can be used on many standard plate readers. The 384-well plate SimpleStep ELISA uses the same quick and simple one-wash protocol as our standard SimpleStep ELISA kits but requires less of your sample to run each plate.

Find more technical information about our 384-well SimpleStep ELISA Microplate here

Find out more about our ELISA-based high-throughput screening solutions here

Uncompromising on sensitivity

SimpleStep ELISA kits drastically reduce assay times without compromising on performance. We compared the sensitivity of SimpleStep ELISA with that of the next best competitor brand of ELISA kits for 75 popular human and mouse proteins. In 56 out of 75 cases, SimpleStep ELISA shows superior sensitivity (figure 2).

Figure 2. We compared SimpleStep ELISA kit sensitivity with a competitor assay across 75 popular protein targets and calculated the ratios. SimpleStep ELISA was more sensitive on average than a competitor ELISA kit.

Tested in biological samples

Every SimpleStep ELISA® kit is validated using multiple biological samples for assay specificity. All secreted serum or plasma-based targets are tested and fall within World Health Organization blood reference ranges. When available, the SimpleStep ELISA kits are calibrated against a known NIBSC international standards and includes a conversion factor for data comparison.

Analyte range

We currently have SimpleStep ELISA® kits to almost 700 targets, including popular proteins like PD-L1, GFP, and fibrinogen, and are continually adding more to the catalog.

SimpleStep ELISA® kits for the top 10 most popular ELISA targets:

Target	Human	Mouse	Rat
IgG*	ab195215		ab189578
Albumin	ab179887** ab227933	ab207620	ab235642
TNF alpha	ab181421	ab208348
IL-6	ab178013	ab222503	ab234570
IFN Gamma	ab174443
IL-1 beta	ab214025	ab197742
GFP	ab171581***
Alpha 1 antitrypsin	ab189579
Pro collagen I alpha	ab210966	ab210579
Fibrinogen	ab241383	ab213478