For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
We use cookies to make our site as useful as possible.
Our Cookie Policy explains how you can opt-out of the cookies we use.
If you continue without changing your cookie settings, we'll assume you’re happy with this.
Treatment of DNA with sodium bisulfite leads to the deamination of cytosine residues and converts them to uracil, while 5-mC residues remain the same. These changes in the DNA sequence provide information about the methylation status of a DNA region with single-nucleotide resolution when detected in subsequent sequencing analysis.
Our bisulfite conversion kits have been optimized to accurately (>99%) convert unmethylated cytosine into uracil. With the ability to use a broad range of sample material, our range of bisulfite conversion kits give you the flexibility needed for your methylation studies.
Use our table below to select the right bisulfite kit for you
Bisulfite Conversion Kit - Whole Cell | Fast Bisulfite Conversion Kit | DNA Isolation & Bisulfite Conversion Kit | Bisulfite-Seq High Sensitivity Kit | Post-Bisulfite DNA Library Preparation Kit | |
Product Code | ab117126 | ab117127 | ab117124 | ab185907 | ab185906 |
Product principle | Direct bisulfite conversion from cells or tissue | Bisulfite conversion of purified DNA | DNA isolation and bisulfite conversion | Simultaneous bisulfite conversion and fragmentation for Illumina® sequencing | Fragmentation for Illumina® sequencing after bisulfite conversion |
Sample input | Cultured cells, tissue samples, embryonic cells / oocytes | Purified DNA | Serum, plasma and body fluids | Purified DNA | Purified bisulfite - converted DNA |
Minimum sample amount | 100 cells / 1 µg tissue | 0.2 ng DNA | 50 pg DNA / 20 cells | 0.5 ng DNA | 1 ng bisulfite-converted DNA |
Protocol Time | 3 hours | 30 minutes | 2.5 hours | 6.5 hours | 5 hours |