• Product name

  • Description

    Rabbit polyclonal to KLB
  • Host species

  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Macaque monkey, Gorilla
  • Immunogen

    Synthetic peptide corresponding to Human KLB aa 700-800 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab127879)

  • Positive control

    • This antibody gave a positive signal in HepG2 whole cell lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal stomach.



Our Abpromise guarantee covers the use of ab106794 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 120 kDa).
IHC-P Use a concentration of 5 µg/ml.


  • Function

    Contributes to the transcriptional repression of cholesterol 7-alpha-hydroxylase (CYP7A1), the rate-limiting enzyme in bile acid synthesis. Probably inactive as a glycosidase. Increases the ability of FGFR1 and FGFR4 to bind FGF21.
  • Sequence similarities

    Belongs to the glycosyl hydrolase 1 family. Klotho subfamily.
  • Domain

    Contains 2 glycosyl hydrolase 1 regions. However, the first region lacks the essential Glu active site residue at position 241, and the second one lacks the essential Glu active site residue at position 889. These domains are therefore predicted to be inactive.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Beta-klotho antibody
    • BetaKlotho antibody
    • BKL antibody
    • KLB antibody
    • KLOTB_HUMAN antibody
    • Klotho beta like protein antibody
    • Klotho beta-like protein antibody
    see all


  • Anti-KLB antibody (ab106794) at 1 µg/ml + HepG2 whole cell lysate at 10 µg

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 120 kDa
    Observed band size: 125 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 39 kDa, 54 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 2 minutes

    KLB contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
  • IHC image of KLB staining in Human normal stomach formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab106794, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


This product has been referenced in:

  • Wang D  et al. Decreased placental and muscular expression of the fibroblast growth factor 19 in gestational diabetes mellitus. J Diabetes Investig N/A:N/A (2018). Read more (PubMed: 29734515) »
  • Liu Z  et al. ßKlotho inhibits androgen/androgen receptor-associated epithelial-mesenchymal transition in prostate cancer through inactivation of ERK1/2 signaling. Oncol Rep 40:217-225 (2018). Read more (PubMed: 29749458) »
See all 4 Publications for this product

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