Anti-KLB antibody (ab233416)
Key features and details
- Rabbit polyclonal to KLB
- Suitable for: IHC-P
- Reacts with: Human
Overview
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Product name
Anti-KLB antibody
See all KLB primary antibodies -
Description
Rabbit polyclonal to KLB -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment (His-tag) corresponding to Human KLB aa 500-650. Expressed in E.coli.
Database link: Q86Z14 -
Positive control
- IHC-P: Human liver, pancreas and kidney tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Research areas
Associated products
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Compatible Secondaries
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233416 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use a concentration of 5 - 20 µg/ml.
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Notes |
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IHC-P
Use a concentration of 5 - 20 µg/ml. |
Target
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Function
Contributes to the transcriptional repression of cholesterol 7-alpha-hydroxylase (CYP7A1), the rate-limiting enzyme in bile acid synthesis. Probably inactive as a glycosidase. Increases the ability of FGFR1 and FGFR4 to bind FGF21. -
Sequence similarities
Belongs to the glycosyl hydrolase 1 family. Klotho subfamily. -
Domain
Contains 2 glycosyl hydrolase 1 regions. However, the first region lacks the essential Glu active site residue at position 241, and the second one lacks the essential Glu active site residue at position 889. These domains are therefore predicted to be inactive. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 152831 Human
- Omim: 611135 Human
- SwissProt: Q86Z14 Human
- Unigene: 90756 Human
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Alternative names
- Beta-klotho antibody
- BetaKlotho antibody
- BKL antibody
see all
Images
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Formalin-fixed, paraffin-embedded human liver tissue stained for KLB using ab233416 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human kidney tissue stained for KLB using ab233416 at 20 μg/ml in immunohistochemical analysis. AEC staining.
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Formalin-fixed, paraffin-embedded human pancreas tissue stained for KLB using ab233416 at 20 μg/ml in immunohistochemical analysis. AEC staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab233416 has been referenced in 1 publication.
- Li H et al. Gypenosides regulate farnesoid X receptor-mediated bile acid and lipid metabolism in a mouse model of non-alcoholic steatohepatitis. Nutr Metab (Lond) 17:34 (2020). PubMed: 32377219