Key features and details
- Rabbit polyclonal to KLB
- Suitable for: IHC-P
- Reacts with: Mouse, Human
Product nameAnti-KLB antibody
See all KLB primary antibodies
DescriptionRabbit polyclonal to KLB
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Mouse, Human
- IHC-P: Human liver, pancreas and kidney tissue.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
Our Abpromise guarantee covers the use of ab233416 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionContributes to the transcriptional repression of cholesterol 7-alpha-hydroxylase (CYP7A1), the rate-limiting enzyme in bile acid synthesis. Probably inactive as a glycosidase. Increases the ability of FGFR1 and FGFR4 to bind FGF21.
Sequence similaritiesBelongs to the glycosyl hydrolase 1 family. Klotho subfamily.
DomainContains 2 glycosyl hydrolase 1 regions. However, the first region lacks the essential Glu active site residue at position 241, and the second one lacks the essential Glu active site residue at position 889. These domains are therefore predicted to be inactive.
Cellular localizationCell membrane.
- Information by UniProt
- Beta-klotho antibody
- BetaKlotho antibody
- BKL antibody
Formalin-fixed, paraffin-embedded human liver tissue stained for KLB using ab233416 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human kidney tissue stained for KLB using ab233416 at 20 μg/ml in immunohistochemical analysis. AEC staining.
Formalin-fixed, paraffin-embedded human pancreas tissue stained for KLB using ab233416 at 20 μg/ml in immunohistochemical analysis. AEC staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233416 has been referenced in 1 publication.
- Li H et al. Gypenosides regulate farnesoid X receptor-mediated bile acid and lipid metabolism in a mouse model of non-alcoholic steatohepatitis. Nutr Metab (Lond) 17:34 (2020). PubMed: 32377219