Overview

  • Product name

  • Description

    Rabbit polyclonal to KLC1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 523-573 of Human Kinesin 2 (NP_891553.2).

  • Positive control

    • HeLa and 293T cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab95882 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 65 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function

    Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. The light chain may function in coupling of cargo to the heavy chain or in the modulation of its ATPase activity.
  • Tissue specificity

    Found in a variety of tissues. Mostly abundant in brain and spine.
  • Sequence similarities

    Belongs to the kinesin light chain family.
    Contains 6 TPR repeats.
  • Post-translational
    modifications

    Isoform I is phosphorylated on Ser-600. Isoform J is phosphorylated on Ser-631.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links

  • Alternative names

    • Kinesin 2 60/70kDa antibody
    • Kinesin light chain 1 antibody
    • Kinesin2 antibody
    • KLC 1 antibody
    • KLC antibody
    • Klc1 antibody
    • KLC1_HUMAN antibody
    • KNS 2 antibody
    • KNS 2A antibody
    • KNS2 antibody
    • KNS2A antibody
    • Medulloblastoma antigen MU MB 2.50 antibody
    • MGC15245 antibody
    see all

Images

  • All lanes : Anti-KLC1 antibody (ab95882) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 65 kDa


    Exposure time: 30 seconds
  • ab95882 at 0.4 µg/ml detecting Kinesin 2 in HeLa whole cell lysate by WB following IP.
    Lane 1: Kinesin 2 with 3 µg/mg of lysate
    Lane 2: IP with an antibody which recognizes an upstream epitope of Kinesin 2
    Lane 3: control IgG.
    In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
    Detection: Chemiluminescence with an exposure time of 30 seconds

References

ab95882 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for contacting us and for your interest in our products.

As far as we are aware, ab95882 has never been tested with samples from human.

All tested species covered by the 6 month guarantee are stated on our datasheets, and these are updated as soon as any new information is brought to our attention.

I have checked the alignment of the immunogen for ab95882 with the rat KCL1 protein, and regrettably this has a low alignment of 54%. Therefore, I am sorry to confirm we would not predict that this antibody would detect in rat. We are therefore unfortunately not able to offer our testing AbTrial discount program in this case.

I am sorry we unfortunately do not have a suitably tested KCL1 protein to suggest on this occasion.

I hope this information is helpful. Please do not hesitate to contact me for any further advice or information.

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