Key features and details
- Rabbit polyclonal to KLC1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-KLC1 antibody
See all KLC1 primary antibodies
DescriptionRabbit polyclonal to KLC1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide, corresponding to a region within amino acids 523-573 of Human Kinesin 2 (NP_891553.2).
- HeLa and 293T cell lysates
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAffinity purified using an epitope specific to Kinesin 2 immobilized on solid support.
Our Abpromise guarantee covers the use of ab95882 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 65 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionKinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. The light chain may function in coupling of cargo to the heavy chain or in the modulation of its ATPase activity.
Tissue specificityFound in a variety of tissues. Mostly abundant in brain and spine.
Sequence similaritiesBelongs to the kinesin light chain family.
Contains 6 TPR repeats.
modificationsIsoform I is phosphorylated on Ser-600. Isoform J is phosphorylated on Ser-631.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- Kinesin 2 60/70kDa antibody
- Kinesin light chain 1 antibody
- Kinesin2 antibody
All lanes : Anti-KLC1 antibody (ab95882) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 65 kDa
Exposure time: 30 seconds
ab95882 at 0.4 µg/ml detecting Kinesin 2 in HeLa whole cell lysate by WB following IP.
Lane 1: Kinesin 2 with 3 µg/mg of lysate
Lane 2: IP with an antibody which recognizes an upstream epitope of Kinesin 2
Lane 3: control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 30 seconds
ab95882 has not yet been referenced specifically in any publications.