Product nameAnti-KLC1 antibody [EPR12441(B)]
See all KLC1 primary antibodies
DescriptionRabbit monoclonal [EPR12441(B)] to KLC1
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IP, Flow Cytmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human KLC1 aa 500 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q07866
- U87-MG, 293T, SH-SY5Y, and HeLa whole cell lysate (ab150035); Human brain tissue; U87-MG and HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab174273 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 61-70 kDa (predicted molecular weight: 65 kDa).|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||1/50 - 1/500.|
|IP||1/10 - 1/100.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionKinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. The light chain may function in coupling of cargo to the heavy chain or in the modulation of its ATPase activity.
Tissue specificityFound in a variety of tissues. Mostly abundant in brain and spine.
Sequence similaritiesBelongs to the kinesin light chain family.
Contains 6 TPR repeats.
modificationsIsoform I is phosphorylated on Ser-600. Isoform J is phosphorylated on Ser-631.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- Kinesin 2 60/70kDa antibody
- Kinesin light chain 1 antibody
- Kinesin2 antibody
All lanes : Anti-KLC1 antibody [EPR12441(B)] (ab174273) at 1/1000 dilution
Lane 1 : U87-MG cell lysate
Lane 2 : 293T cell lysate
Lane 3 : SH-SY5Y cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 65 kDa
Immunocytochemistry/Immunofluorescence analysis of MCF-7 (human breast carcinoma) labelling KLC1 with purified ab174273 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).
Control: PBS only
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling KLC1 with ab174273 at 1/50 dilution.
Western blot analysis on immunoprecipitation pellet from SH-SY5Y cell lysate using ab174273 at 1/10 dilution.
Immunofluorescent analysis of U87-MG cells labeling KLC1 with ab174273 at 1/50 dilution.
Flow Cytometric analysis of permeabilized HeLa cells labeling KLC1 with ab174273 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
This product has been referenced in:
- Malikov V & Naghavi MH Localized Phosphorylation of a Kinesin-1 Adaptor by a Capsid-Associated Kinase Regulates HIV-1 Motility and Uncoating. Cell Rep 20:2792-2799 (2017). Read more (PubMed: 28930676) »
- Wilson MH & Holzbaur EL Nesprins anchor kinesin-1 motors to the nucleus to drive nuclear distribution in muscle cells. Development 142:218-28 (2015). Read more (PubMed: 25516977) »