Recombinant Anti-KLC1 antibody [EPR12441(B)] (ab174273)
![Western blot - Anti-KLC1 antibody [EPR12441(B)] (ab174273)](https://www.abcam.com/ps/products/174/ab174273/Images/ab174273-391467-anti-klc1-antibody-epr12441-western-blot-human-knockout-lysate.jpg "Western blot - Anti-KLC1 antibody [EPR12441(B)] (ab174273)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12441(B)] to KLC1
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IP
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-KLC1 antibody [EPR12441(B)]
See all KLC1 primary antibodies -
Description
Rabbit monoclonal [EPR12441(B)] to KLC1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- U87-MG, 293T, SH-SY5Y, and HeLa whole cell lysate (ab150035); Human brain tissue; U87-MG and HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12441(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab174273 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 61-70 kDa (predicted molecular weight: 65 kDa).
|
| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
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| ICC/IF |
1/50 - 1/500.
|
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| IP |
1/10 - 1/100.
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| Notes |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Detects a band of approximately 61-70 kDa (predicted molecular weight: 65 kDa). |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
|
ICC/IF
1/50 - 1/500. |
|
IP
1/10 - 1/100. |
Target
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Function
Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. The light chain may function in coupling of cargo to the heavy chain or in the modulation of its ATPase activity. -
Tissue specificity
Found in a variety of tissues. Mostly abundant in brain and spine. -
Sequence similarities
Belongs to the kinesin light chain family.
Contains 6 TPR repeats. -
Post-translational
modificationsIsoform I is phosphorylated on Ser-600. Isoform J is phosphorylated on Ser-631. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 3831 Human
- Entrez Gene: 16593 Mouse
- Entrez Gene: 171041 Rat
- Omim: 600025 Human
- SwissProt: Q07866 Human
- SwissProt: O88447 Mouse
- SwissProt: P37285 Rat
- Unigene: 20107 Human
see all -
Alternative names
- Kinesin 2 60/70kDa antibody
- Kinesin light chain 1 antibody
- Kinesin2 antibody
see all
Images
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Western blot - Anti-KLC1 antibody [EPR12441(B)] (ab174273)All lanes : Anti-KLC1 antibody [EPR12441(B)] (ab174273) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : KLC1 knockout HeLa cell lysate
Lane 3 : U-87 MG cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 65 kDa
Observed band size: 65 kDaLanes 1-3: Merged signal (red and green). Green - ab174273 observed at 65 kDa. Red - loading control ab8245 observed at 36 kDa.
ab174273 Anti-KLC1 antibody [EPR12441(B)] was shown to specifically react with KLC1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265284 (knockout cell lysate ab257496) was used. Wild-type and KLC1 knockout samples were subjected to SDS-PAGE. ab174273 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunocytochemistry/ Immunofluorescence - Anti-KLC1 antibody [EPR12441(B)] (ab174273)](https://www.abcam.com/ps/products/174/ab174273/Images/ab174273-265183-anti-klc1-antibody-epr12441b-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-KLC1 antibody [EPR12441(B)] (ab174273)Immunocytochemistry/Immunofluorescence analysis of MCF-7 (human breast carcinoma) labelling KLC1 with purified ab174273 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).
Control: PBS only
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLC1 antibody [EPR12441(B)] (ab174273)](https://www.abcam.com/ps/products/174/ab174273/Images/ab174273-199162-anti-klc1-antibody-epr12441b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLC1 antibody [EPR12441(B)] (ab174273)Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling KLC1 with ab174273 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Western blot - Anti-KLC1 antibody [EPR12441(B)] (ab174273)](https://www.abcam.com/ps/products/174/ab174273/Images/ab174273-199163-anti-klc1-antibody-epr12441b-western-blot.jpg)
Western blot - Anti-KLC1 antibody [EPR12441(B)] (ab174273)All lanes : Anti-KLC1 antibody [EPR12441(B)] (ab174273) at 1/1000 dilution
Lane 1 : U87-MG cell lysate
Lane 2 : 293T cell lysate
Lane 3 : SH-SY5Y cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 65 kDa -
![Immunoprecipitation - Anti-KLC1 antibody [EPR12441(B)] (ab174273)](https://www.abcam.com/ps/products/174/ab174273/Images/ab174273-199165-anti-klc1-antibody-epr12441b-immunoprecipitation.jpg)
Immunoprecipitation - Anti-KLC1 antibody [EPR12441(B)] (ab174273)Western blot analysis on immunoprecipitation pellet from SH-SY5Y cell lysate using ab174273 at 1/10 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-KLC1 antibody [EPR12441(B)] (ab174273)](https://www.abcam.com/ps/products/174/ab174273/Images/ab174273-199159-anti-klc1-antibody-epr12441b-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-KLC1 antibody [EPR12441(B)] (ab174273)Immunofluorescent analysis of U87-MG cells labeling KLC1 with ab174273 at 1/50 dilution.
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![Flow Cytometry (Intracellular) - Anti-KLC1 antibody [EPR12441(B)] (ab174273)](https://www.abcam.com/ps/products/174/ab174273/Images/ab174273-199158-anti-klc1-antibody-epr12441b-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-KLC1 antibody [EPR12441(B)] (ab174273)Intracellular flow cytometric analysis of permeabilized HeLa cells labeling KLC1with ab174273 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
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![Anti-KLC1 antibody [EPR12441(B)] (ab174273)](https://www.abcam.com/ps/products/174/ab174273/Images/ab174273-8-benefits-of-recombinant-antibodies.png)
Anti-KLC1 antibody [EPR12441(B)] (ab174273)
Protocols
Datasheets and documents
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Datasheet download
References (5)
ab174273 has been referenced in 5 publications.
- Giovarelli M et al. Drp1 overexpression induces desmin disassembling and drives kinesin-1 activation promoting mitochondrial trafficking in skeletal muscle. Cell Death Differ 27:2383-2401 (2020). PubMed: 32042098
- Franchini DM et al. Microtubule-Driven Stress Granule Dynamics Regulate Inhibitory Immune Checkpoint Expression in T Cells. Cell Rep 26:94-107.e7 (2019). PubMed: 30605689
- Moamer A et al. A role for kinesin-1 subunits KIF5B/KLC1 in regulating epithelial mesenchymal plasticity in breast tumorigenesis. EBioMedicine 45:92-107 (2019). PubMed: 31204277
- Malikov V & Naghavi MH Localized Phosphorylation of a Kinesin-1 Adaptor by a Capsid-Associated Kinase Regulates HIV-1 Motility and Uncoating. Cell Rep 20:2792-2799 (2017). PubMed: 28930676
- Wilson MH & Holzbaur EL Nesprins anchor kinesin-1 motors to the nucleus to drive nuclear distribution in muscle cells. Development 142:218-28 (2015). PubMed: 25516977
