Anti-KLC1 antibody [EPR12441(B)] (ab174273)
- Datasheet
- References (2)
- Protocols
Overview
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Product nameAnti-KLC1 antibody [EPR12441(B)]
See all KLC1 primary antibodies -
DescriptionRabbit monoclonal [EPR12441(B)] to KLC1
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IP, Flow Cytmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human KLC1 aa 500 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q07866 -
Positive control
- U87-MG, 293T, SH-SY5Y, and HeLa whole cell lysate (ab150035); Human brain tissue; U87-MG and HeLa cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR12441(B)
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab174273 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 61-70 kDa (predicted molecular weight: 65 kDa). | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| ICC/IF | 1/50 - 1/500. | |
| IP | 1/10 - 1/100. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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FunctionKinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. The light chain may function in coupling of cargo to the heavy chain or in the modulation of its ATPase activity.
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Tissue specificityFound in a variety of tissues. Mostly abundant in brain and spine.
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Sequence similaritiesBelongs to the kinesin light chain family.
Contains 6 TPR repeats. -
Post-translational
modificationsIsoform I is phosphorylated on Ser-600. Isoform J is phosphorylated on Ser-631. -
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
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Database links
- Entrez Gene: 3831 Human
- Entrez Gene: 16593 Mouse
- Entrez Gene: 171041 Rat
- Omim: 600025 Human
- SwissProt: Q07866 Human
- SwissProt: O88447 Mouse
- SwissProt: P37285 Rat
- Unigene: 20107 Human
see all -
Alternative names
- Kinesin 2 60/70kDa antibody
- Kinesin light chain 1 antibody
- Kinesin2 antibody
see all
Images
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![Western blot - Anti-KLC1 antibody [EPR12441(B)] (ab174273)](https://www.abcam.com/ps/products/174/ab174273/Images/ab174273-199163-ab174273.jpg)
Western blot - Anti-KLC1 antibody [EPR12441(B)] (ab174273)All lanes : Anti-KLC1 antibody [EPR12441(B)] (ab174273) at 1/1000 dilution
Lane 1 : U87-MG cell lysate
Lane 2 : 293T cell lysate
Lane 3 : SH-SY5Y cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 65 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-KLC1 antibody [EPR12441(B)] (ab174273)](https://www.abcam.com/ps/products/174/ab174273/Images/ab174273-265183-ab174273-MCF7methanol500.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-KLC1 antibody [EPR12441(B)] (ab174273)Immunocytochemistry/Immunofluorescence analysis of MCF-7 (human breast carcinoma) labelling KLC1 with purified ab174273 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).
Control: PBS only
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLC1 antibody [EPR12441(B)] (ab174273)](https://www.abcam.com/ps/products/174/ab174273/Images/ab174273-199162-ab174273b.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLC1 antibody [EPR12441(B)] (ab174273)Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling KLC1 with ab174273 at 1/50 dilution.
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![Immunoprecipitation - Anti-KLC1 antibody [EPR12441(B)] (ab174273)](https://www.abcam.com/ps/products/174/ab174273/Images/ab174273-199165-ab174273e.jpg)
Immunoprecipitation - Anti-KLC1 antibody [EPR12441(B)] (ab174273)Western blot analysis on immunoprecipitation pellet from SH-SY5Y cell lysate using ab174273 at 1/10 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-KLC1 antibody [EPR12441(B)] (ab174273)](https://www.abcam.com/ps/products/174/ab174273/Images/ab174273-199159-ab174273c.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-KLC1 antibody [EPR12441(B)] (ab174273)Immunofluorescent analysis of U87-MG cells labeling KLC1 with ab174273 at 1/50 dilution.
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![Flow Cytometry - Anti-KLC1 antibody [EPR12441(B)] (ab174273)](https://www.abcam.com/ps/products/174/ab174273/Images/ab174273-199158-ab174273d.jpg)
Flow Cytometry - Anti-KLC1 antibody [EPR12441(B)] (ab174273)Flow Cytometric analysis of permeabilized HeLa cells labeling KLC1 with ab174273 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
Protocols
Datasheets and documents
References
This product has been referenced in:
- Malikov V & Naghavi MH Localized Phosphorylation of a Kinesin-1 Adaptor by a Capsid-Associated Kinase Regulates HIV-1 Motility and Uncoating. Cell Rep 20:2792-2799 (2017). Read more (PubMed: 28930676) »
- Wilson MH & Holzbaur EL Nesprins anchor kinesin-1 motors to the nucleus to drive nuclear distribution in muscle cells. Development 142:218-28 (2015). Read more (PubMed: 25516977) »
