Product nameKLC1 overexpression 293T lysate (whole cell)
DescriptionKLC1 overexpression lysate
ab94276 is a 293T cell transfected lysate in which Human Kinesin 2 has been transiently over-expressed using a pCMV-Kinesin 2 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferpH: 6.80
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol
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BackgroundFunction: Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. The light chain may function in coupling of cargo to the heavy chain or in the modulation of its ATPase activity. Tissue specificity: Found in a variety of tissues. Mostly abundant in brain and spine. Similarity: Belongs to the kinesin light chain family. Contains 6 TPR repeats. PTM: Isoform I is phosphorylated on Ser-600. Isoform J is phosphorylated on Ser-631.
Our Abpromise guarantee covers the use of ab94276 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94276 at 15µg/lane on an SDS-PAGE gel.
All lanes : Anti-KLC1 antibody (ab88095) at 1/500 dilution
Lane 1 :
KLC1 overexpression 293T lysate (whole cell) (ab94276)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94276 has not yet been referenced specifically in any publications.