Key features and details
- Rabbit polyclonal to KLC2
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-KLC2 antibody
See all KLC2 primary antibodies
DescriptionRabbit polyclonal to KLC2
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
Synthetic peptide corresponding to Mouse KLC2 aa 100-200 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following Mouse tissue lysates: Brain; Spinal Cord; Sciatic Nerve; Testis.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab154212 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 66 kDa).|
FunctionKinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. The light chain may function in coupling of cargo to the heavy chain or in the modulation of its ATPase activity.
Sequence similaritiesBelongs to the kinesin light chain family.
Contains 6 TPR repeats.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- Kinesin light chain 2 antibody
- KLC 2 antibody
- KLC2 antibody
- KLC2_HUMAN antibody
All lanes : Anti-KLC2 antibody (ab154212) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Spinal Cord (Mouse) Tissue Lysate
Lane 3 : Sciatic Nerve (Mouse) Tissue Lysate
Lane 4 : Testis (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 66 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Additional bands at: 103 kDa, 37 kDa, 45 kDa, 99 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 seconds
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab154212 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
ab154212 has not yet been referenced specifically in any publications.