Key features and details
- Rabbit polyclonal to KLC2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-KLC2 antibody
See all KLC2 primary antibodies
DescriptionRabbit polyclonal to KLC2
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Orangutan
Synthetic peptide, corresponding to a region between amino acids 150-200 of Human KLC2 (NP_001128247.1).
- HeLa cell lysate
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAffinity purified using an epitope specific to KLC2 immobilized on solid support.
Our Abpromise guarantee covers the use of ab95881 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 69 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionKinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. The light chain may function in coupling of cargo to the heavy chain or in the modulation of its ATPase activity.
Sequence similaritiesBelongs to the kinesin light chain family.
Contains 6 TPR repeats.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- Kinesin light chain 2 antibody
- KLC 2 antibody
- KLC2 antibody
- KLC2_HUMAN antibody
All lanes : Anti-KLC2 antibody (ab95881) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 69 kDa
Exposure time: 3 minutes
ab95881 at 0.4 µg/ml detecting KLC2 in HeLa whole cell lysate by WB following IP.
Lane 1: KLC2 with 3 µg/mg of lysate
Lane 2: IP with an antibody which recognizes a downstream epitope of KLC2
Lane 3: control IgG
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 30 seconds.
ab95881 has been referenced in 1 publication.
- Carpentier DC et al. Vaccinia virus protein complex F12/E2 interacts with kinesin light chain isoform 2 to engage the kinesin-1 motor complex. PLoS Pathog 11:e1004723 (2015). WB, IF . PubMed: 25760349