Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11149(2)] to KLC3
- Suitable for: ICC/IF, WB, IHC-P, Flow Cyt, IP
- Reacts with: Mouse, Human
Product nameAnti-KLC3 antibody [EPR11149(2)]
See all KLC3 primary antibodies
DescriptionRabbit monoclonal [EPR11149(2)] to KLC3
Tested applicationsSuitable for: ICC/IF, WB, IHC-P, Flow Cyt, IPmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human KLC3 aa 350-450. The exact sequence is proprietary.
Database link: Q6P597
- Jurkat, U87-MG, HepG2 and BxPC-3 cell lysates; Human liver tissue; HepG2 and Jurkat cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab180523 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionKinesin is a microtubule-associated force-producing protein that may play a role in organelle transport.
Sequence similaritiesBelongs to the kinesin light chain family.
Contains 5 TPR repeats.
DomainThe heptad repeat (HR) motif is sufficient for interaction with kinesin heavy (KHL) chains and ODF1. The TPR region is involved in mitochondrial binding.
Cellular localizationCytoplasm > cytoskeleton. Elongating spermatid tail midpiece, localized in outer dense fibers (ODFs) and associates with mitochondria.
- Information by UniProt
- Kinesin light chain 2 antibody
- Kinesin light chain 3 antibody
- KLC2 antibody
All lanes : Anti-KLC3 antibody [EPR11149(2)] (ab180523) at 1/50000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : U87-MG cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : BxPC-3 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 55 kDa
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling KLC3 with ab180523 at 1/250 dilution followed by ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG secondary and counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunofluorescent analysis of HepG2 cells (4% paraformaldehyde-fixed) labeling KLC3 with ab180523 at 1/1000 dilution followed by Goat anti-rabbit IgG (Dylight 555) at 1/250 dilution and counterstained with DAPI.
Western blot analysis of immunoprecipitation pellet from BxPC-3 cell lysate immunoprecipitated using ab180523 at 1/40 dilution (lane 1). Negative control (lane 2). Rabbit TrueBlot®:Anti-Rabbit IgG HRP at 1/1500 dilution was used as secondary.
Flow cytometric analysis of Jurkat cells (2% paraformaldehyde-fixed) labeling KLC3 with ab180523 at 1/10 dilution. Goat anti-rabbit IgG (FITC) at 1/75 dilution was used as the secondary antibody. Rabbit monoclonal IgG was used as the isotype control.
ab180523 has been referenced in 1 publication.
- Georgiades P et al. The flexibility and dynamics of the tubules in the endoplasmic reticulum. Sci Rep 7:16474 (2017). PubMed: 29184084