Overview

  • Product name
    Anti-KLC3 antibody [EPR11149(2)]
    See all KLC3 primary antibodies
  • Description
    Rabbit monoclonal [EPR11149(2)] to KLC3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-P, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human KLC3 aa 350-450. The exact sequence is proprietary.
    Database link: Q6P597

  • Positive control
    • Jurkat, U87-MG, HepG2 and BxPC-3 cell lysates; Human liver tissue; HepG2 and Jurkat cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR11149(2)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab180523 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000.
WB 1/10000 - 1/50000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
IHC-P 1/100 - 1/250.
Flow Cyt 1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP 1/40.

Target

  • Function
    Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport.
  • Sequence similarities
    Belongs to the kinesin light chain family.
    Contains 5 TPR repeats.
  • Domain
    The heptad repeat (HR) motif is sufficient for interaction with kinesin heavy (KHL) chains and ODF1. The TPR region is involved in mitochondrial binding.
  • Cellular localization
    Cytoplasm > cytoskeleton. Elongating spermatid tail midpiece, localized in outer dense fibers (ODFs) and associates with mitochondria.
  • Information by UniProt
  • Database links
  • Alternative names
    • Kinesin light chain 2 antibody
    • Kinesin light chain 3 antibody
    • KLC2 antibody
    • KLC2 like antibody
    • KLC2-like antibody
    • KLC2L antibody
    • KLC3 antibody
    • KLC3_HUMAN antibody
    • KLCt antibody
    • KNS2B antibody
    see all

Images

  • All lanes : Anti-KLC3 antibody [EPR11149(2)] (ab180523) at 1/50000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : U87-MG cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : BxPC-3 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 55 kDa

  • Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling KLC3 with ab180523 at 1/250 dilution followed by ImmunoHistoprobe (Ready to use)  HRP Polymer for Rabbit IgG secondary and counter stained with Hematoxylin.

  • Immunofluorescent analysis of HepG2 cells (4% paraformaldehyde-fixed) labeling KLC3 with ab180523 at 1/1000 dilution followed by Goat anti-rabbit IgG (Dylight 555) at 1/250 dilution and counterstained with DAPI.

  • Western blot analysis of immunoprecipitation pellet from BxPC-3 cell lysate immunoprecipitated using ab180523 at 1/40 dilution (lane 1). Negative control (lane 2). Rabbit TrueBlot®:Anti-Rabbit IgG HRP at 1/1500 dilution was used as secondary.

  • Flow cytometric analysis of Jurkat cells (2% paraformaldehyde-fixed) labeling KLC3 with ab180523 at 1/10 dilution. Goat anti-rabbit IgG (FITC) at 1/75 dilution was used as the secondary antibody. Rabbit monoclonal IgG was used as the isotype control.

References

This product has been referenced in:

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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