Key features and details
- Rabbit polyclonal to KLC4
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-KLC4 antibody
DescriptionRabbit polyclonal to KLC4
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow, Dog, Macaque monkey
Synthetic peptide corresponding to Human KLC4 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in Mouse and Rat brain tissue lysates as well as HeLa and Jurkat whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab101190 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 69 kDa).|
FunctionKinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. The light chain may function in coupling of cargo to the heavy chain or in the modulation of its ATPase activity.
Sequence similaritiesBelongs to the kinesin light chain family.
Contains 7 TPR repeats.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- Kinesin light chain 4 antibody
- kinesin like 8 antibody
- Kinesin like protein 8 antibody
All lanes : Anti-KLC4 antibody (ab101190) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 69 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
The predicted molecular weight of KLC4 is 69 kDa (SwissProt), however we expect to observe a banding pattern at 75 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
ab101190 has not yet been referenced specifically in any publications.