Overview

  • Product name

    Anti-KLF10 antibody [EPR12102(2)]
    See all KLF10 primary antibodies
  • Description

    Rabbit monoclonal [EPR12102(2)] to KLF10
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human KLF10 aa 50-150. The exact sequence is proprietary.
    Database link: Q13118

  • Positive control

    • Jurkat, HeLa, K652 and HepG2 whole cell lysate (ab7900).
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab184182 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).

Target

  • Function

    Transcriptional repressor which binds to the consensus sequence 5'-GGTGTG-3'. Plays a role in the regulation of the circadian clock; binds to the GC box sequence in the promoter of the core clock component ARTNL/BMAL1 and represses its transcriptional activity. Regulates the circadian expression of genes involved in lipogenesis, gluconeogenesis, and glycolysis in the liver. Represses the expression of PCK2, a rate-limiting step enzyme of gluconeogenesis (By similarity). May play a role in the cell cycle regulation.
  • Sequence similarities

    Belongs to the Sp1 C2H2-type zinc-finger protein family.
    Contains 3 C2H2-type zinc fingers.
  • Post-translational
    modifications

    Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Early growth response, alpha antibody
    • Early growth response, alpha like antibody
    • EGR A antibody
    • EGR alpha antibody
    • EGR(A) antibody
    • EGR-alpha antibody
    • EGRA antibody
    • Egral antibody
    • EGRalpha antibody
    • Gdnfif antibody
    • KLF 10 antibody
    • KLF10 antibody
    • KLF10_HUMAN antibody
    • Krueppel like factor 10 antibody
    • Krueppel like factor10 antibody
    • Krueppel-like factor 10 antibody
    • Kruppel like factor 10 antibody
    • mGIF antibody
    • TGF-beta- inducible early gene antibody
    • TGFB inducible early growth response 1 antibody
    • TGFB inducible early growth response antibody
    • TGFB inducible early growth response protein 1 antibody
    • TGFB-inducible early growth response protein 1 antibody
    • TIEG 1 antibody
    • TIEG antibody
    • TIEG-1 antibody
    • TIEG1 antibody
    • Transforming growth factor beta inducible early growth response antibody
    • Transforming growth factor beta inducible early growth response protein 1 antibody
    • Transforming growth factor-beta-inducible early growth response protein 1 antibody
    • Zinc finger transcription factor TIEG antibody
    see all

Images

  • All lanes : Anti-KLF10 antibody [EPR12102(2)] (ab184182) at 1/10000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 53 kDa

  • All lanes : Anti-KLF10 antibody [EPR12102(2)] (ab184182) at 1/2000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : K652 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 53 kDa

References

This product has been referenced in:

  • Mishra VK  et al. Krüppel-like Transcription Factor KLF10 Suppresses TGFß-Induced Epithelial-to-Mesenchymal Transition via a Negative Feedback Mechanism. Cancer Res 77:2387-2400 (2017). Read more (PubMed: 28249899) »
See 1 Publication for this product

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