Anti-KLF12 antibody (ab129459)
Key features and details
- Rabbit polyclonal to KLF12
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KLF12 antibody
See all KLF12 primary antibodies -
Description
Rabbit polyclonal to KLF12 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
Immunogen
A synthetic peptide corresponding to a region between amino acids 25-75 of Human KLF12 (NP_009180.3).
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Positive control
- Jurkat, 293T and HeLa whole cell lysate (ab150035).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab129459 is affinity purified using an epitope specific to KLF12 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab129459 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 44 kDa.
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IP | (1) |
Use at 2-10 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 44 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
Confers strong transcriptional repression to the AP-2-alpha gene. Binds to a regulatory element (A32) in the AP-2-alpha gene promoter. -
Sequence similarities
Belongs to the Sp1 C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 11278 Human
- Omim: 607531 Human
- SwissProt: Q9Y4X4 Human
- Unigene: 373857 Human
- Unigene: 729350 Human
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Alternative names
- 2700063E05Rik antibody
- AI225908 antibody
- AI315654 antibody
see all
Images
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All lanes : Anti-KLF12 antibody (ab129459) at 0.1 µg/ml
Lane 1 : Jurkat whole cell lysate at 50 µg
Lane 2 : Jurkat whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : HeLa whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 44 kDa
Exposure time: 3 minutes -
Detection of KLF12 by Western Blot of Immunoprecipitates.
ab129459 at 0.4µg/ml staining KLF12 in Jurkat whole cell lysate immunoprecipitated using ab129459 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab129459 has been referenced in 7 publications.
- Yang J et al. Silencing of circCYP51A1 represses cell progression and glycolysis by regulating miR-490-3p/KLF12 axis in osteosarcoma under hypoxia. J Bone Oncol 37:100455 (2022). PubMed: 36276300
- Chen H et al. circ_0000467 promotes the proliferation, metastasis, and angiogenesis in colorectal cancer cells through regulating KLF12 expression by sponging miR-4766-5p. Open Med (Wars) 16:1415-1427 (2021). PubMed: 34616917
- Zhou D et al. Long non-coding RNA NEAT1 transported by extracellular vesicles contributes to breast cancer development by sponging microRNA-141-3p and regulating KLF12. Cell Biosci 11:68 (2021). PubMed: 33820555
- Zhang W et al. Downregulation of Circ_0071589 Suppresses Cisplatin Resistance in Colorectal Cancer by Regulating the MiR-526b-3p/KLF12 Axis. Cancer Manag Res 13:2717-2731 (2021). PubMed: 33790646
- Xie C et al. Circular RNA hsa_circ_0003496 Contributes to Tumorigenesis and Chemoresistance in Osteosarcoma Through Targeting (microRNA) miR-370/Krüppel-Like Factor 12 Axis. Cancer Manag Res 12:8229-8240 (2020). PubMed: 32982419
- Mao S et al. Exosomal miR-141 promotes tumor angiogenesis via KLF12 in small cell lung cancer. J Exp Clin Cancer Res 39:193 (2020). PubMed: 32958011
- Ding L et al. Dysregulation of Krüppel-like factor 12 in the development of endometrial cancer. Gynecol Oncol 152:177-184 (2019). PubMed: 30482501