Key features and details
- Rabbit polyclonal to KLF15
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-KLF15 antibody
See all KLF15 primary antibodies
DescriptionRabbit polyclonal to KLF15
Specificityab22851 reacts with Krueppel-like factor 15 (KLF15).
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
- HepG2 cell lysate and human liver.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab22851 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use at an assay dependent concentration.|
FunctionTranscriptional activator. Binds to the GA element of the CLCNKA promoter.
Tissue specificityHighly expressed in liver, followed by heart, skeletal muscle, and kidney. Not expressed in bone marrow or lymphoid tissues.
Sequence similaritiesBelongs to the Sp1 C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers.
- Information by UniProt
- Kidney enriched Kruppel like factor antibody
- Kidney-enriched krueppel-like factor antibody
- KKLF antibody
Anti-KLF15 antibody (ab22851) at 4 µg/ml + HepG2 cell lysate.
HRP conjugated anti-Rabbit IgG should be diluted in 1/50,000 - 100,000
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 44 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling KLF15 with ab22851 at a concentration of 4-8µg/ml. Positive stained hepatocytes marked by arrows. Magnification X400.
ab22851 has been referenced in 1 publication.
- Li Y et al. KLF15 Regulates the Expression of MMP-3 in Human Chondrocytes. J Interferon Cytokine Res 38:356-362 (2018). PubMed: 30036111