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Rabbit polyclonal to KLF15
ab22851 reacts with Krueppel-like factor 15 (KLF15).
Predicted to work with:
Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
Synthetic peptide within Human KLF15 aa 45-94 (N terminal). The exact sequence is proprietary.
HepG2 cell lysate and human liver.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
Protein A purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
Transcriptional activator. Binds to the GA element of the CLCNKA promoter.
Highly expressed in liver, followed by heart, skeletal muscle, and kidney. Not expressed in bone marrow or lymphoid tissues.
Belongs to the Sp1 C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers.
Information by UniProt
Kidney enriched Kruppel like factor antibody
Kidney-enriched krueppel-like factor antibody
Western blot - Anti-KLF15 antibody (ab22851)
Anti-KLF15 antibody (ab22851) at 4 µg/ml + HepG2 cell lysate.
Secondary HRP conjugated anti-Rabbit IgG should be diluted in 1/50,000 - 100,000 Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 44 kDa Observed band size: 44 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF15 antibody (ab22851)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling KLF15 with ab22851 at a concentration of 4-8µg/ml. Positive stained hepatocytes marked by arrows. Magnification X400.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"