Overview

  • Product name
    Anti-KLF15 antibody
    See all KLF15 primary antibodies
  • Description
    Goat polyclonal to KLF15
  • Host species
    Goat
  • Tested applications
    Suitable for: IP, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Pig
  • Immunogen

    Synthetic peptide: VDHLLPVDENFSS, corresponding to N terminal amino acids 2-14 of Human KLF15.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Human Liver or HepG2 lysate
  • General notes

    Principal Names – KLF15; Kruppel-like factor 15; KKLF; KKLF protein; kidney-enriched Kruppel-like factor Official Gene Symbol - KLF15 GenBank Accession Number – NP_054798 LocusLink ID - 28999 (human); 66277 (mouse); 85497 (rat)

     

    We no longer guarantee this product for mouse because of recent complaints. The immunogen however shares 92% sequence homology with mouse so we still predict the antibody will cross-react. We will welcome any feedback from customers.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2647 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration. PubMed: 24407292
WB Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 44 kDa).Can be blocked with Human KLF15 peptide (ab22927).

Approx 48kDa band observed in lysates of hepatoblastoma cell line HepG2 (calculated MW of 44.0kDa according to NP_054798.1). Older batches detected a band of approximately 65 kDa in Human Liver lysate(predicted molecular weight: 46 kDa). Note the higher than expected size is in agreement with previous Western results (Mol Cell Biol 2000 Oct;20(19):7319-31).
IHC-P Use at an assay dependent concentration. PubMed: 26378916

Target

Images

  • Western blot - Anti-KLF15 antibody (ab2647)
    Western blot - Anti-KLF15 antibody (ab2647)
    ab2647 staining (2µg/ml) of Human Liver lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 12 hours.  Detected by western blot using chemiluminescence. ab2647 staining (1 µg/ml) of Mouse skeletal muscle lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 12 hours. Detected by western blot using chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF15 antibody (ab2647)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF15 antibody (ab2647)Image is from Hoon-Kim et al., PLoS Genet., 11(9), Fig 8. doi: 10.1371/journal.pgen.1005537

    The level of KLF15 in human endometrium without (b) and with (d) endometriosis were performed by immunohistochemical analysis. Paraffin-embedded tissues were cut at 5 μm and mounted on silane-coated slides, deparaffinized, and rehydrated in a graded alcohol series before blocking with 10% normal goat serum in PBS (pH 7.5) and incubating with ab2647 diluted 1:5000 in 10% normal goat serum in PBS (pH 7.5) overnight at 4°C

  • Western blot - Anti-KLF15 antibody (ab2647)
    Western blot - Anti-KLF15 antibody (ab2647)
    Lane 1 : Anti-KLF15 antibody (ab2647) at 1 µg/ml
    Lane 2 : Anti-KLF15 antibody (ab2647) at 0.5 µg/ml

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 44 kDa

References

This product has been referenced in:
  • Liang N  et al. Immunosuppressive effects of hydroxychloroquine and artemisinin combination therapy via the nuclear factor-?B signaling pathway in lupus nephritis mice. Exp Ther Med 15:2436-2442 (2018). WB ; Mouse . Read more (PubMed: 29456648) »
  • Walter LM  et al. Interventions Targeting Glucocorticoid-Krüppel-like Factor 15-Branched-Chain Amino Acid Signaling Improve Disease Phenotypes in Spinal Muscular Atrophy Mice. EBioMedicine 31:226-242 (2018). WB ; Human . Read more (PubMed: 29735415) »

See all 11 Publications for this product

Publishing research using ab2647? Please let us know so that we can cite the reference in this datasheet.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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