Anti-KLF15 antibody (ab2647)

Product name

Anti-KLF15 antibody
See all KLF15 primary antibodies
Description

Goat polyclonal to KLF15
Host species

Goat
Tested applications

Suitable for: IP, WB, IHC-Pmore details
Species reactivity

Reacts with: Human
Predicted to work with: Mouse, Rat, Pig
Immunogen

Synthetic peptide: VDHLLPVDENFSS, corresponding to N terminal amino acids 2-14 of Human KLF15.

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Positive control
- Human Liver or HepG2 lysate
General notes

Principal Names – KLF15; Kruppel-like factor 15; KKLF; KKLF protein; kidney-enriched Kruppel-like factor Official Gene Symbol - KLF15 GenBank Accession Number – NP_054798 LocusLink ID - 28999 (human); 66277 (mouse); 85497 (rat)

We no longer guarantee this product for mouse because of recent complaints. The immunogen however shares 92% sequence homology with mouse so we still predict the antibody will cross-react. We will welcome any feedback from customers.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration information loading...
Purity

Immunogen affinity purified
Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality

Polyclonal
Isotype

IgG
Research areas

Our Abpromise guarantee covers the use of ab2647 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
IP		Use at an assay dependent concentration. PubMed: 24407292
WB		Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 44 kDa).Can be blocked with Human KLF15 peptide (ab22927). Approx 48kDa band observed in lysates of hepatoblastoma cell line HepG2 (calculated MW of 44.0kDa according to NP_054798.1). Older batches detected a band of approximately 65 kDa in Human Liver lysate(predicted molecular weight: 46 kDa). Note the higher than expected size is in agreement with previous Western results (Mol Cell Biol 2000 Oct;20(19):7319-31).
IHC-P		Use at an assay dependent concentration. PubMed: 26378916

Function

Transcriptional activator. Binds to the GA element of the CLCNKA promoter.
Tissue specificity

Highly expressed in liver, followed by heart, skeletal muscle, and kidney. Not expressed in bone marrow or lymphoid tissues.
Sequence similarities

Belongs to the Sp1 C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers.
Cellular localization

Nucleus.
Target information above from: UniProt accession Q9UIH9 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 28999 Human
- Entrez Gene: 66277 Mouse
- Entrez Gene: 85497 Rat
- GenBank: NP_054798.1 Human
- Omim: 606465 Human
- SwissProt: Q9UIH9 Human
- SwissProt: Q9EPW2 Mouse
- Unigene: 272215 Human
- Unigene: 41389 Mouse
- Unigene: 22556 Rat
see all
Alternative names
- Kidney enriched Kruppel like factor antibody
- Kidney-enriched krueppel-like factor antibody
- KKLF antibody
- KKLF protein antibody
- KLF 15 antibody
- KLF15 antibody
- KLF15_HUMAN antibody
- Krueppel-like factor 15 antibody
- Kruppel like factor 15 antibody
see all

Western blot - Anti-KLF15 antibody (ab2647)

ab2647 staining (2µg/ml) of Human Liver lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 12 hours. Detected by western blot using chemiluminescence. ab2647 staining (1 µg/ml) of Mouse skeletal muscle lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 12 hours. Detected by western blot using chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF15 antibody (ab2647)Image is from Hoon-Kim et al., PLoS Genet., 11(9), Fig 8. doi: 10.1371/journal.pgen.1005537

The level of KLF15 in human endometrium without (b) and with (d) endometriosis were performed by immunohistochemical analysis. Paraffin-embedded tissues were cut at 5 μm and mounted on silane-coated slides, deparaffinized, and rehydrated in a graded alcohol series before blocking with 10% normal goat serum in PBS (pH 7.5) and incubating with ab2647 diluted 1:5000 in 10% normal goat serum in PBS (pH 7.5) overnight at 4°C
Western blot - Anti-KLF15 antibody (ab2647)

Lane 1 : Anti-KLF15 antibody (ab2647) at 1 µg/ml
Lane 2 : Anti-KLF15 antibody (ab2647) at 0.5 µg/ml

Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate

Lysates/proteins at 35 µg per lane.

Predicted band size: 44 kDa

Liang N et al. Immunosuppressive effects of hydroxychloroquine and artemisinin combination therapy via the nuclear factor-?B signaling pathway in lupus nephritis mice. Exp Ther Med 15:2436-2442 (2018). WB ; Mouse . Read more (PubMed: 29456648) »
Walter LM et al. Interventions Targeting Glucocorticoid-Krüppel-like Factor 15-Branched-Chain Amino Acid Signaling Improve Disease Phenotypes in Spinal Muscular Atrophy Mice. EBioMedicine 31:226-242 (2018). WB ; Human . Read more (PubMed: 29735415) »

Publishing research using ab2647? Please let us know so that we can cite the reference in this datasheet.

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