Anti-KLF15 antibody (ab2647)
- Datasheet
- References (12)
- Protocols
Overview
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Product nameAnti-KLF15 antibody
See all KLF15 primary antibodies -
DescriptionGoat polyclonal to KLF15
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Host speciesGoat
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Tested applicationsSuitable for: IP, WB, IHC-Pmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Pig -
Immunogen
Synthetic peptide: VDHLLPVDENFSS, corresponding to N terminal amino acids 2-14 of Human KLF15.
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Positive control
- Human Liver or HepG2 lysate
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General notes
Principal Names – KLF15; Kruppel-like factor 15; KKLF; KKLF protein; kidney-enriched Kruppel-like factor Official Gene Symbol - KLF15 GenBank Accession Number – NP_054798 LocusLink ID - 28999 (human); 66277 (mouse); 85497 (rat)
We no longer guarantee this product for mouse because of recent complaints. The immunogen however shares 92% sequence homology with mouse so we still predict the antibody will cross-react. We will welcome any feedback from customers.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 -
Concentration information loading...
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PurityImmunogen affinity purified
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Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab2647 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP | Use at an assay dependent concentration. PubMed: 24407292 | |
WB | Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 44 kDa).Can be blocked with Human KLF15 peptide (ab22927). Approx 48kDa band observed in lysates of hepatoblastoma cell line HepG2 (calculated MW of 44.0kDa according to NP_054798.1). Older batches detected a band of approximately 65 kDa in Human Liver lysate(predicted molecular weight: 46 kDa). Note the higher than expected size is in agreement with previous Western results (Mol Cell Biol 2000 Oct;20(19):7319-31). |
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IHC-P | Use at an assay dependent concentration. PubMed: 26378916 |
Target
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FunctionTranscriptional activator. Binds to the GA element of the CLCNKA promoter.
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Tissue specificityHighly expressed in liver, followed by heart, skeletal muscle, and kidney. Not expressed in bone marrow or lymphoid tissues.
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Sequence similaritiesBelongs to the Sp1 C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers. -
Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 28999 Human
- Entrez Gene: 66277 Mouse
- Entrez Gene: 85497 Rat
- GenBank: NP_054798.1 Human
- Omim: 606465 Human
- SwissProt: Q9UIH9 Human
- SwissProt: Q9EPW2 Mouse
- Unigene: 272215 Human
see all -
Alternative names
- Kidney enriched Kruppel like factor antibody
- Kidney-enriched krueppel-like factor antibody
- KKLF antibody
see all
Images
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ab2647 staining (2
µ g/ml) of Human Liver lysate (RIPA buffer, 30µ g total protein per lane). Primary incubated for 12 hours. Detected by western blot using chemiluminescence. ab2647 staining (1 µg/ml) of Mouse skeletal muscle lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 12 hours. Detected by western blot using chemiluminescence. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF15 antibody (ab2647)Image is from Hoon-Kim et al., PLoS Genet., 11(9), Fig 8. doi: 10.1371/journal.pgen.1005537
The level of KLF15 in human endometrium without (b) and with (d) endometriosis were performed by immunohistochemical analysis. Paraffin-embedded tissues were cut at 5 μm and mounted on silane-coated slides, deparaffinized, and rehydrated in a graded alcohol series before blocking with 10% normal goat serum in PBS (pH 7.5) and incubating with ab2647 diluted 1:5000 in 10% normal goat serum in PBS (pH 7.5) overnight at 4°C
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Lane 1 : Anti-KLF15 antibody (ab2647) at 1 µg/ml
Lane 2 : Anti-KLF15 antibody (ab2647) at 0.5 µg/ml
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 44 kDa
Datasheets and documents
References
This product has been referenced in:
- Liang N et al. Immunosuppressive effects of hydroxychloroquine and artemisinin combination therapy via the nuclear factor-?B signaling pathway in lupus nephritis mice. Exp Ther Med 15:2436-2442 (2018). WB ; Mouse . Read more (PubMed: 29456648) »
- Walter LM et al. Interventions Targeting Glucocorticoid-Krüppel-like Factor 15-Branched-Chain Amino Acid Signaling Improve Disease Phenotypes in Spinal Muscular Atrophy Mice. EBioMedicine 31:226-242 (2018). WB ; Human . Read more (PubMed: 29735415) »